Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3671 and Pf1N1B4_3672 are separated by 206 nucleotides Pf1N1B4_3672 and Pf1N1B4_3673 overlap by 4 nucleotides
Pf1N1B4_3671 - Glycine betaine/L-proline ABC transporter, glycine betaine/L-proline- binding/permease protein, at 4,076,225 to 4,077,076
_3671
Pf1N1B4_3672 - small component of pyruvate transporter (yjcH-like) (from data) , at 4,077,283 to 4,077,594
_3672
Pf1N1B4_3673 - large component of pyruvate transporter (actP-like) (from data) , at 4,077,591 to 4,079,249
_3673
Position (kb)
4077
4078 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4076.321 kb on + strand, within Pf1N1B4_3671 at 4076.325 kb on + strand at 4076.325 kb on + strand, within Pf1N1B4_3671 at 4076.325 kb on + strand, within Pf1N1B4_3671 at 4076.325 kb on + strand, within Pf1N1B4_3671 at 4076.325 kb on + strand, within Pf1N1B4_3671 at 4076.325 kb on + strand, within Pf1N1B4_3671 at 4076.325 kb on + strand, within Pf1N1B4_3671 at 4076.326 kb on - strand, within Pf1N1B4_3671 at 4076.326 kb on - strand, within Pf1N1B4_3671 at 4076.326 kb on - strand, within Pf1N1B4_3671 at 4076.326 kb on - strand, within Pf1N1B4_3671 at 4076.501 kb on + strand, within Pf1N1B4_3671 at 4076.502 kb on - strand, within Pf1N1B4_3671 at 4076.582 kb on + strand, within Pf1N1B4_3671 at 4076.582 kb on + strand, within Pf1N1B4_3671 at 4076.582 kb on + strand, within Pf1N1B4_3671 at 4076.582 kb on + strand, within Pf1N1B4_3671 at 4076.583 kb on - strand, within Pf1N1B4_3671 at 4076.599 kb on + strand, within Pf1N1B4_3671 at 4076.599 kb on + strand at 4076.599 kb on + strand, within Pf1N1B4_3671 at 4076.599 kb on + strand, within Pf1N1B4_3671 at 4076.600 kb on - strand, within Pf1N1B4_3671 at 4076.699 kb on + strand, within Pf1N1B4_3671 at 4076.699 kb on + strand, within Pf1N1B4_3671 at 4076.699 kb on + strand, within Pf1N1B4_3671 at 4076.699 kb on + strand, within Pf1N1B4_3671 at 4076.700 kb on - strand, within Pf1N1B4_3671 at 4076.700 kb on - strand, within Pf1N1B4_3671 at 4076.700 kb on - strand, within Pf1N1B4_3671 at 4076.700 kb on - strand, within Pf1N1B4_3671 at 4076.700 kb on - strand, within Pf1N1B4_3671 at 4076.883 kb on + strand, within Pf1N1B4_3671 at 4076.887 kb on + strand, within Pf1N1B4_3671 at 4076.887 kb on + strand, within Pf1N1B4_3671 at 4076.887 kb on + strand, within Pf1N1B4_3671 at 4076.887 kb on + strand, within Pf1N1B4_3671 at 4076.887 kb on + strand, within Pf1N1B4_3671 at 4076.888 kb on - strand, within Pf1N1B4_3671 at 4077.094 kb on - strand at 4077.124 kb on + strand at 4077.125 kb on - strand at 4077.150 kb on - strand at 4077.197 kb on + strand at 4077.197 kb on + strand at 4077.198 kb on - strand at 4077.198 kb on - strand at 4077.201 kb on - strand at 4077.203 kb on + strand at 4077.204 kb on - strand at 4077.204 kb on - strand at 4077.204 kb on - strand at 4077.204 kb on - strand at 4077.235 kb on + strand at 4077.236 kb on - strand at 4077.236 kb on - strand at 4077.236 kb on - strand at 4077.236 kb on - strand at 4077.256 kb on + strand at 4077.257 kb on - strand at 4077.257 kb on - strand at 4077.257 kb on - strand at 4077.257 kb on - strand at 4077.257 kb on - strand at 4077.257 kb on - strand at 4077.299 kb on - strand at 4077.392 kb on - strand, within Pf1N1B4_3672 at 4077.465 kb on + strand, within Pf1N1B4_3672 at 4077.465 kb on + strand, within Pf1N1B4_3672 at 4077.465 kb on + strand, within Pf1N1B4_3672 at 4077.465 kb on + strand, within Pf1N1B4_3672 at 4077.465 kb on + strand, within Pf1N1B4_3672 at 4077.465 kb on + strand, within Pf1N1B4_3672 at 4077.465 kb on + strand, within Pf1N1B4_3672 at 4077.466 kb on - strand, within Pf1N1B4_3672 at 4077.526 kb on + strand, within Pf1N1B4_3672 at 4077.526 kb on + strand, within Pf1N1B4_3672 at 4077.527 kb on - strand, within Pf1N1B4_3672 at 4077.527 kb on - strand, within Pf1N1B4_3672 at 4077.527 kb on - strand, within Pf1N1B4_3672 at 4077.527 kb on - strand, within Pf1N1B4_3672 at 4077.527 kb on - strand, within Pf1N1B4_3672 at 4077.747 kb on + strand at 4077.747 kb on + strand at 4077.748 kb on - strand at 4077.765 kb on - strand at 4077.783 kb on + strand, within Pf1N1B4_3673 at 4077.783 kb on + strand, within Pf1N1B4_3673 at 4077.783 kb on + strand, within Pf1N1B4_3673 at 4077.786 kb on + strand at 4077.786 kb on + strand, within Pf1N1B4_3673 at 4077.843 kb on + strand, within Pf1N1B4_3673 at 4077.843 kb on + strand at 4077.843 kb on + strand, within Pf1N1B4_3673 at 4077.843 kb on + strand at 4077.843 kb on + strand, within Pf1N1B4_3673 at 4077.844 kb on - strand, within Pf1N1B4_3673 at 4077.844 kb on - strand, within Pf1N1B4_3673 at 4077.844 kb on - strand, within Pf1N1B4_3673 at 4077.844 kb on - strand, within Pf1N1B4_3673 at 4077.844 kb on - strand, within Pf1N1B4_3673 at 4077.869 kb on + strand at 4077.870 kb on - strand, within Pf1N1B4_3673 at 4077.912 kb on + strand, within Pf1N1B4_3673 at 4077.913 kb on - strand, within Pf1N1B4_3673 at 4077.987 kb on + strand, within Pf1N1B4_3673 at 4077.987 kb on + strand, within Pf1N1B4_3673 at 4077.988 kb on - strand, within Pf1N1B4_3673 at 4077.988 kb on - strand, within Pf1N1B4_3673 at 4077.988 kb on - strand, within Pf1N1B4_3673 at 4078.179 kb on + strand, within Pf1N1B4_3673 at 4078.179 kb on + strand, within Pf1N1B4_3673 at 4078.179 kb on + strand, within Pf1N1B4_3673 at 4078.179 kb on + strand, within Pf1N1B4_3673 at 4078.179 kb on + strand, within Pf1N1B4_3673 at 4078.179 kb on + strand, within Pf1N1B4_3673 at 4078.179 kb on + strand, within Pf1N1B4_3673 at 4078.179 kb on + strand, within Pf1N1B4_3673 at 4078.179 kb on + strand at 4078.180 kb on - strand, within Pf1N1B4_3673 at 4078.180 kb on - strand, within Pf1N1B4_3673 at 4078.334 kb on + strand, within Pf1N1B4_3673 at 4078.494 kb on + strand, within Pf1N1B4_3673 at 4078.494 kb on + strand, within Pf1N1B4_3673 at 4078.494 kb on + strand, within Pf1N1B4_3673 at 4078.494 kb on + strand, within Pf1N1B4_3673 at 4078.494 kb on + strand, within Pf1N1B4_3673 at 4078.495 kb on - strand, within Pf1N1B4_3673 at 4078.495 kb on - strand, within Pf1N1B4_3673 at 4078.495 kb on - strand, within Pf1N1B4_3673 at 4078.515 kb on + strand, within Pf1N1B4_3673 at 4078.515 kb on + strand, within Pf1N1B4_3673 at 4078.516 kb on - strand, within Pf1N1B4_3673 at 4078.521 kb on + strand at 4078.521 kb on + strand, within Pf1N1B4_3673 at 4078.521 kb on + strand, within Pf1N1B4_3673 at 4078.521 kb on + strand, within Pf1N1B4_3673 at 4078.522 kb on - strand, within Pf1N1B4_3673 at 4078.522 kb on - strand, within Pf1N1B4_3673 at 4078.571 kb on + strand, within Pf1N1B4_3673 at 4078.572 kb on - strand, within Pf1N1B4_3673 at 4078.583 kb on + strand, within Pf1N1B4_3673 at 4078.583 kb on + strand, within Pf1N1B4_3673 at 4078.584 kb on - strand, within Pf1N1B4_3673
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 remove 4,076,321 + Pf1N1B4_3671 0.11 +0.3 4,076,325 + +0.6 4,076,325 + Pf1N1B4_3671 0.12 -0.4 4,076,325 + Pf1N1B4_3671 0.12 +0.2 4,076,325 + Pf1N1B4_3671 0.12 -1.9 4,076,325 + Pf1N1B4_3671 0.12 +0.9 4,076,325 + Pf1N1B4_3671 0.12 +0.3 4,076,325 + Pf1N1B4_3671 0.12 -0.6 4,076,326 - Pf1N1B4_3671 0.12 +1.6 4,076,326 - Pf1N1B4_3671 0.12 -0.4 4,076,326 - Pf1N1B4_3671 0.12 -0.5 4,076,326 - Pf1N1B4_3671 0.12 +0.6 4,076,501 + Pf1N1B4_3671 0.32 -0.0 4,076,502 - Pf1N1B4_3671 0.33 -0.3 4,076,582 + Pf1N1B4_3671 0.42 +0.6 4,076,582 + Pf1N1B4_3671 0.42 -1.7 4,076,582 + Pf1N1B4_3671 0.42 +0.9 4,076,582 + Pf1N1B4_3671 0.42 +0.6 4,076,583 - Pf1N1B4_3671 0.42 +0.3 4,076,599 + Pf1N1B4_3671 0.44 -0.1 4,076,599 + +1.0 4,076,599 + Pf1N1B4_3671 0.44 +0.0 4,076,599 + Pf1N1B4_3671 0.44 +0.1 4,076,600 - Pf1N1B4_3671 0.44 -0.4 4,076,699 + Pf1N1B4_3671 0.56 -0.6 4,076,699 + Pf1N1B4_3671 0.56 -0.1 4,076,699 + Pf1N1B4_3671 0.56 -0.4 4,076,699 + Pf1N1B4_3671 0.56 -0.4 4,076,700 - Pf1N1B4_3671 0.56 -0.2 4,076,700 - Pf1N1B4_3671 0.56 +0.0 4,076,700 - Pf1N1B4_3671 0.56 +1.9 4,076,700 - Pf1N1B4_3671 0.56 -0.3 4,076,700 - Pf1N1B4_3671 0.56 -1.6 4,076,883 + Pf1N1B4_3671 0.77 -1.2 4,076,887 + Pf1N1B4_3671 0.78 -0.5 4,076,887 + Pf1N1B4_3671 0.78 -1.6 4,076,887 + Pf1N1B4_3671 0.78 +1.2 4,076,887 + Pf1N1B4_3671 0.78 -1.4 4,076,887 + Pf1N1B4_3671 0.78 -1.6 4,076,888 - Pf1N1B4_3671 0.78 -2.0 4,077,094 - +0.2 4,077,124 + -0.2 4,077,125 - +0.0 4,077,150 - +0.1 4,077,197 + +0.2 4,077,197 + +1.3 4,077,198 - +0.3 4,077,198 - -0.9 4,077,201 - -1.4 4,077,203 + +0.5 4,077,204 - -1.1 4,077,204 - -0.7 4,077,204 - +0.2 4,077,204 - -0.2 4,077,235 + +1.2 4,077,236 - -1.4 4,077,236 - -1.1 4,077,236 - -0.2 4,077,236 - -0.4 4,077,256 + +1.8 4,077,257 - +1.6 4,077,257 - +1.1 4,077,257 - -1.2 4,077,257 - -0.5 4,077,257 - -1.4 4,077,257 - +0.4 4,077,299 - -1.4 4,077,392 - Pf1N1B4_3672 0.35 -1.2 4,077,465 + Pf1N1B4_3672 0.58 -0.6 4,077,465 + Pf1N1B4_3672 0.58 +0.3 4,077,465 + Pf1N1B4_3672 0.58 -1.2 4,077,465 + Pf1N1B4_3672 0.58 -0.9 4,077,465 + Pf1N1B4_3672 0.58 -0.4 4,077,465 + Pf1N1B4_3672 0.58 +0.7 4,077,465 + Pf1N1B4_3672 0.58 -0.4 4,077,466 - Pf1N1B4_3672 0.59 -0.6 4,077,526 + Pf1N1B4_3672 0.78 -1.6 4,077,526 + Pf1N1B4_3672 0.78 -1.6 4,077,527 - Pf1N1B4_3672 0.78 -0.4 4,077,527 - Pf1N1B4_3672 0.78 +1.2 4,077,527 - Pf1N1B4_3672 0.78 -1.0 4,077,527 - Pf1N1B4_3672 0.78 -0.5 4,077,527 - Pf1N1B4_3672 0.78 -1.0 4,077,747 + -0.5 4,077,747 + -0.5 4,077,748 - -0.8 4,077,765 - +0.2 4,077,783 + Pf1N1B4_3673 0.12 -2.1 4,077,783 + Pf1N1B4_3673 0.12 -0.4 4,077,783 + Pf1N1B4_3673 0.12 -0.3 4,077,786 + +0.6 4,077,786 + Pf1N1B4_3673 0.12 +0.8 4,077,843 + Pf1N1B4_3673 0.15 -1.2 4,077,843 + -0.4 4,077,843 + Pf1N1B4_3673 0.15 -2.3 4,077,843 + +0.6 4,077,843 + Pf1N1B4_3673 0.15 +0.0 4,077,844 - Pf1N1B4_3673 0.15 -1.2 4,077,844 - Pf1N1B4_3673 0.15 +0.3 4,077,844 - Pf1N1B4_3673 0.15 -2.1 4,077,844 - Pf1N1B4_3673 0.15 +0.0 4,077,844 - Pf1N1B4_3673 0.15 -0.3 4,077,869 + +1.6 4,077,870 - Pf1N1B4_3673 0.17 +0.6 4,077,912 + Pf1N1B4_3673 0.19 -1.0 4,077,913 - Pf1N1B4_3673 0.19 -1.2 4,077,987 + Pf1N1B4_3673 0.24 -0.1 4,077,987 + Pf1N1B4_3673 0.24 +0.1 4,077,988 - Pf1N1B4_3673 0.24 +0.3 4,077,988 - Pf1N1B4_3673 0.24 +0.6 4,077,988 - Pf1N1B4_3673 0.24 -1.2 4,078,179 + Pf1N1B4_3673 0.35 -1.9 4,078,179 + Pf1N1B4_3673 0.35 +0.0 4,078,179 + Pf1N1B4_3673 0.35 +0.6 4,078,179 + Pf1N1B4_3673 0.35 -1.6 4,078,179 + Pf1N1B4_3673 0.35 +0.3 4,078,179 + Pf1N1B4_3673 0.35 -0.3 4,078,179 + Pf1N1B4_3673 0.35 -2.2 4,078,179 + Pf1N1B4_3673 0.35 -0.7 4,078,179 + -0.1 4,078,180 - Pf1N1B4_3673 0.36 +0.1 4,078,180 - Pf1N1B4_3673 0.36 -2.2 4,078,334 + Pf1N1B4_3673 0.45 +1.6 4,078,494 + Pf1N1B4_3673 0.54 +2.2 4,078,494 + Pf1N1B4_3673 0.54 -2.5 4,078,494 + Pf1N1B4_3673 0.54 +0.1 4,078,494 + Pf1N1B4_3673 0.54 +0.3 4,078,494 + Pf1N1B4_3673 0.54 +0.3 4,078,495 - Pf1N1B4_3673 0.54 -0.2 4,078,495 - Pf1N1B4_3673 0.54 +0.9 4,078,495 - Pf1N1B4_3673 0.54 -2.2 4,078,515 + Pf1N1B4_3673 0.56 -0.4 4,078,515 + Pf1N1B4_3673 0.56 -1.1 4,078,516 - Pf1N1B4_3673 0.56 +0.9 4,078,521 + -0.7 4,078,521 + Pf1N1B4_3673 0.56 -0.6 4,078,521 + Pf1N1B4_3673 0.56 -1.4 4,078,521 + Pf1N1B4_3673 0.56 -0.1 4,078,522 - Pf1N1B4_3673 0.56 -0.7 4,078,522 - Pf1N1B4_3673 0.56 -0.8 4,078,571 + Pf1N1B4_3673 0.59 -1.4 4,078,572 - Pf1N1B4_3673 0.59 +0.1 4,078,583 + Pf1N1B4_3673 0.60 -0.0 4,078,583 + Pf1N1B4_3673 0.60 -1.1 4,078,584 - Pf1N1B4_3673 0.60 -2.9
Or see this region's nucleotide sequence