Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3517 and Pf1N1B4_3518 are separated by 15 nucleotides Pf1N1B4_3518 and Pf1N1B4_3519 are separated by 31 nucleotides Pf1N1B4_3519 and Pf1N1B4_3520 overlap by 4 nucleotides Pf1N1B4_3520 and Pf1N1B4_3521 are separated by 194 nucleotides
Pf1N1B4_3517 - hypothetical protein, at 3,925,609 to 3,925,827
_3517
Pf1N1B4_3518 - Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94), at 3,925,843 to 3,926,769
_3518
Pf1N1B4_3519 - Lipase (EC 3.1.1.3), at 3,926,801 to 3,927,148
_3519
Pf1N1B4_3520 - Phosphohistidine phosphatase SixA, at 3,927,145 to 3,927,597
_3520
Pf1N1B4_3521 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 3,927,792 to 3,929,459
_3521
Position (kb)
3926
3927
3928 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3926.606 kb on + strand, within Pf1N1B4_3518 at 3926.607 kb on - strand, within Pf1N1B4_3518 at 3926.638 kb on + strand, within Pf1N1B4_3518 at 3926.638 kb on + strand, within Pf1N1B4_3518 at 3926.639 kb on - strand, within Pf1N1B4_3518 at 3926.639 kb on - strand, within Pf1N1B4_3518 at 3926.639 kb on - strand, within Pf1N1B4_3518 at 3926.639 kb on - strand, within Pf1N1B4_3518 at 3926.639 kb on - strand, within Pf1N1B4_3518 at 3926.708 kb on + strand at 3926.745 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.768 kb on - strand at 3926.768 kb on - strand at 3926.771 kb on + strand at 3926.781 kb on + strand at 3926.781 kb on + strand at 3926.781 kb on + strand at 3926.781 kb on + strand at 3926.781 kb on + strand at 3926.782 kb on - strand at 3926.782 kb on - strand at 3926.782 kb on - strand at 3926.782 kb on - strand at 3926.828 kb on + strand at 3926.828 kb on + strand at 3926.828 kb on + strand at 3926.828 kb on + strand at 3926.867 kb on + strand, within Pf1N1B4_3519 at 3926.867 kb on + strand, within Pf1N1B4_3519 at 3926.867 kb on + strand at 3926.919 kb on + strand, within Pf1N1B4_3519 at 3926.919 kb on + strand, within Pf1N1B4_3519 at 3926.919 kb on + strand, within Pf1N1B4_3519 at 3926.919 kb on + strand, within Pf1N1B4_3519 at 3926.920 kb on - strand, within Pf1N1B4_3519 at 3926.931 kb on - strand, within Pf1N1B4_3519 at 3926.942 kb on + strand, within Pf1N1B4_3519 at 3927.144 kb on + strand at 3927.159 kb on - strand at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.287 kb on - strand, within Pf1N1B4_3520 at 3927.419 kb on + strand, within Pf1N1B4_3520 at 3927.478 kb on + strand, within Pf1N1B4_3520 at 3927.479 kb on - strand, within Pf1N1B4_3520 at 3927.479 kb on - strand, within Pf1N1B4_3520 at 3927.479 kb on - strand, within Pf1N1B4_3520 at 3927.639 kb on + strand at 3927.640 kb on - strand at 3927.727 kb on - strand at 3927.828 kb on + strand at 3927.828 kb on + strand at 3927.828 kb on + strand at 3927.828 kb on + strand at 3927.829 kb on - strand at 3927.829 kb on - strand at 3927.829 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 remove 3,926,606 + Pf1N1B4_3518 0.82 -1.2 3,926,607 - Pf1N1B4_3518 0.82 -1.7 3,926,638 + Pf1N1B4_3518 0.86 -0.4 3,926,638 + Pf1N1B4_3518 0.86 -1.9 3,926,639 - Pf1N1B4_3518 0.86 -0.7 3,926,639 - Pf1N1B4_3518 0.86 -1.4 3,926,639 - Pf1N1B4_3518 0.86 -0.2 3,926,639 - Pf1N1B4_3518 0.86 -1.7 3,926,639 - Pf1N1B4_3518 0.86 +0.6 3,926,708 + +1.6 3,926,745 + -0.2 3,926,767 + -1.9 3,926,767 + -0.7 3,926,767 + -0.8 3,926,767 + -0.1 3,926,767 + +0.6 3,926,767 + +0.7 3,926,767 + +0.2 3,926,767 + +0.6 3,926,767 + -1.1 3,926,768 - +1.9 3,926,768 - -0.5 3,926,771 + +1.2 3,926,781 + -1.0 3,926,781 + -0.0 3,926,781 + +2.6 3,926,781 + +1.6 3,926,781 + +0.3 3,926,782 - +0.8 3,926,782 - +0.0 3,926,782 - +1.2 3,926,782 - -0.7 3,926,828 + -1.9 3,926,828 + -0.3 3,926,828 + -0.9 3,926,828 + +0.1 3,926,867 + Pf1N1B4_3519 0.19 +2.3 3,926,867 + Pf1N1B4_3519 0.19 -1.9 3,926,867 + +0.3 3,926,919 + Pf1N1B4_3519 0.34 +1.2 3,926,919 + Pf1N1B4_3519 0.34 -0.2 3,926,919 + Pf1N1B4_3519 0.34 +1.6 3,926,919 + Pf1N1B4_3519 0.34 +0.3 3,926,920 - Pf1N1B4_3519 0.34 +0.5 3,926,931 - Pf1N1B4_3519 0.37 -0.7 3,926,942 + Pf1N1B4_3519 0.41 -2.4 3,927,144 + -2.1 3,927,159 - -0.5 3,927,286 + Pf1N1B4_3520 0.31 -0.4 3,927,286 + Pf1N1B4_3520 0.31 +0.6 3,927,286 + Pf1N1B4_3520 0.31 +0.7 3,927,286 + Pf1N1B4_3520 0.31 +0.0 3,927,286 + Pf1N1B4_3520 0.31 -0.3 3,927,286 + Pf1N1B4_3520 0.31 +0.9 3,927,287 - Pf1N1B4_3520 0.31 +0.9 3,927,419 + Pf1N1B4_3520 0.60 -0.6 3,927,478 + Pf1N1B4_3520 0.74 -1.0 3,927,479 - Pf1N1B4_3520 0.74 -2.1 3,927,479 - Pf1N1B4_3520 0.74 +0.0 3,927,479 - Pf1N1B4_3520 0.74 -1.8 3,927,639 + -0.3 3,927,640 - -1.3 3,927,727 - +0.5 3,927,828 + +0.6 3,927,828 + +1.0 3,927,828 + -2.2 3,927,828 + -2.6 3,927,829 - -1.2 3,927,829 - -0.4 3,927,829 - -1.3
Or see this region's nucleotide sequence