Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3514 and Pf1N1B4_3515 overlap by 4 nucleotides Pf1N1B4_3515 and Pf1N1B4_3516 are separated by 1 nucleotides
Pf1N1B4_3514 - Sensor histidine kinase/response regulator, at 3,920,592 to 3,922,493
_3514
Pf1N1B4_3515 - FIG00953467: hypothetical protein, at 3,922,490 to 3,923,422
_3515
Pf1N1B4_3516 - TonB-dependent receptor; Outer membrane receptor for ferrienterochelin and colicins, at 3,923,424 to 3,925,547
_3516
Position (kb)
3922
3923
3924 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3921.862 kb on + strand, within Pf1N1B4_3514 at 3921.863 kb on - strand, within Pf1N1B4_3514 at 3921.953 kb on + strand, within Pf1N1B4_3514 at 3921.954 kb on - strand, within Pf1N1B4_3514 at 3921.954 kb on - strand, within Pf1N1B4_3514 at 3921.954 kb on - strand, within Pf1N1B4_3514 at 3922.057 kb on + strand, within Pf1N1B4_3514 at 3922.057 kb on + strand, within Pf1N1B4_3514 at 3922.057 kb on + strand, within Pf1N1B4_3514 at 3922.057 kb on + strand, within Pf1N1B4_3514 at 3922.057 kb on + strand, within Pf1N1B4_3514 at 3922.058 kb on - strand, within Pf1N1B4_3514 at 3922.058 kb on - strand, within Pf1N1B4_3514 at 3922.058 kb on - strand, within Pf1N1B4_3514 at 3922.198 kb on + strand, within Pf1N1B4_3514 at 3922.198 kb on + strand, within Pf1N1B4_3514 at 3922.198 kb on + strand, within Pf1N1B4_3514 at 3922.198 kb on + strand, within Pf1N1B4_3514 at 3922.198 kb on + strand, within Pf1N1B4_3514 at 3922.198 kb on + strand, within Pf1N1B4_3514 at 3922.198 kb on + strand, within Pf1N1B4_3514 at 3922.198 kb on + strand, within Pf1N1B4_3514 at 3922.198 kb on + strand, within Pf1N1B4_3514 at 3922.199 kb on - strand, within Pf1N1B4_3514 at 3922.199 kb on - strand, within Pf1N1B4_3514 at 3922.199 kb on - strand, within Pf1N1B4_3514 at 3922.199 kb on - strand, within Pf1N1B4_3514 at 3922.199 kb on - strand, within Pf1N1B4_3514 at 3922.227 kb on + strand, within Pf1N1B4_3514 at 3922.227 kb on + strand at 3922.227 kb on + strand, within Pf1N1B4_3514 at 3922.228 kb on - strand, within Pf1N1B4_3514 at 3922.242 kb on + strand, within Pf1N1B4_3514 at 3922.242 kb on + strand, within Pf1N1B4_3514 at 3922.243 kb on - strand, within Pf1N1B4_3514 at 3922.397 kb on + strand at 3922.398 kb on - strand at 3922.463 kb on + strand at 3922.552 kb on + strand at 3922.583 kb on + strand at 3922.583 kb on + strand at 3922.584 kb on - strand, within Pf1N1B4_3515 at 3922.584 kb on - strand, within Pf1N1B4_3515 at 3922.683 kb on + strand, within Pf1N1B4_3515 at 3922.683 kb on + strand, within Pf1N1B4_3515 at 3922.683 kb on + strand, within Pf1N1B4_3515 at 3922.683 kb on + strand, within Pf1N1B4_3515 at 3922.683 kb on + strand, within Pf1N1B4_3515 at 3922.684 kb on - strand, within Pf1N1B4_3515 at 3922.684 kb on - strand, within Pf1N1B4_3515 at 3922.684 kb on - strand, within Pf1N1B4_3515 at 3922.687 kb on + strand, within Pf1N1B4_3515 at 3922.752 kb on + strand, within Pf1N1B4_3515 at 3922.752 kb on + strand, within Pf1N1B4_3515 at 3922.752 kb on + strand, within Pf1N1B4_3515 at 3922.752 kb on + strand, within Pf1N1B4_3515 at 3922.752 kb on + strand, within Pf1N1B4_3515 at 3922.791 kb on + strand, within Pf1N1B4_3515 at 3922.792 kb on - strand, within Pf1N1B4_3515 at 3923.165 kb on + strand, within Pf1N1B4_3515 at 3923.425 kb on + strand at 3923.425 kb on + strand at 3923.425 kb on + strand at 3923.425 kb on + strand at 3923.425 kb on + strand at 3923.425 kb on + strand at 3923.425 kb on + strand at 3923.425 kb on + strand at 3923.426 kb on - strand at 3923.426 kb on - strand at 3923.426 kb on - strand at 3923.426 kb on - strand at 3923.449 kb on + strand at 3923.449 kb on + strand at 3923.449 kb on + strand at 3923.449 kb on + strand at 3923.450 kb on - strand at 3923.470 kb on + strand at 3923.470 kb on + strand at 3923.470 kb on + strand at 3923.470 kb on + strand at 3923.470 kb on + strand at 3923.471 kb on - strand at 3923.620 kb on + strand at 3923.620 kb on + strand at 3923.620 kb on + strand at 3923.620 kb on + strand at 3923.620 kb on + strand at 3923.621 kb on - strand at 3923.621 kb on - strand at 3923.623 kb on + strand at 3923.623 kb on + strand at 3923.623 kb on + strand at 3923.623 kb on + strand at 3923.624 kb on - strand at 3923.661 kb on + strand, within Pf1N1B4_3516 at 3923.734 kb on + strand, within Pf1N1B4_3516 at 3923.734 kb on + strand, within Pf1N1B4_3516 at 3923.734 kb on + strand, within Pf1N1B4_3516 at 3923.734 kb on + strand, within Pf1N1B4_3516 at 3923.734 kb on + strand, within Pf1N1B4_3516 at 3923.734 kb on + strand, within Pf1N1B4_3516 at 3923.734 kb on + strand, within Pf1N1B4_3516 at 3923.734 kb on + strand, within Pf1N1B4_3516 at 3923.735 kb on - strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.741 kb on - strand, within Pf1N1B4_3516 at 3923.741 kb on - strand, within Pf1N1B4_3516 at 3923.741 kb on - strand, within Pf1N1B4_3516 at 3923.833 kb on + strand, within Pf1N1B4_3516 at 3923.833 kb on + strand, within Pf1N1B4_3516 at 3923.833 kb on + strand, within Pf1N1B4_3516 at 3923.833 kb on + strand, within Pf1N1B4_3516 at 3923.834 kb on - strand, within Pf1N1B4_3516 at 3923.834 kb on - strand, within Pf1N1B4_3516 at 3923.881 kb on + strand, within Pf1N1B4_3516 at 3923.932 kb on + strand, within Pf1N1B4_3516 at 3923.932 kb on + strand, within Pf1N1B4_3516 at 3923.998 kb on + strand, within Pf1N1B4_3516 at 3923.998 kb on + strand, within Pf1N1B4_3516 at 3924.049 kb on + strand, within Pf1N1B4_3516 at 3924.103 kb on + strand, within Pf1N1B4_3516 at 3924.104 kb on - strand, within Pf1N1B4_3516 at 3924.139 kb on + strand, within Pf1N1B4_3516 at 3924.139 kb on + strand, within Pf1N1B4_3516 at 3924.193 kb on + strand, within Pf1N1B4_3516 at 3924.193 kb on + strand, within Pf1N1B4_3516 at 3924.193 kb on + strand, within Pf1N1B4_3516 at 3924.193 kb on + strand, within Pf1N1B4_3516 at 3924.194 kb on - strand, within Pf1N1B4_3516 at 3924.194 kb on - strand at 3924.289 kb on + strand, within Pf1N1B4_3516 at 3924.289 kb on + strand, within Pf1N1B4_3516 at 3924.289 kb on + strand, within Pf1N1B4_3516 at 3924.289 kb on + strand, within Pf1N1B4_3516 at 3924.289 kb on + strand, within Pf1N1B4_3516 at 3924.290 kb on - strand, within Pf1N1B4_3516 at 3924.290 kb on - strand, within Pf1N1B4_3516 at 3924.290 kb on - strand, within Pf1N1B4_3516 at 3924.290 kb on - strand, within Pf1N1B4_3516 at 3924.319 kb on + strand, within Pf1N1B4_3516 at 3924.319 kb on + strand, within Pf1N1B4_3516 at 3924.385 kb on + strand, within Pf1N1B4_3516
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 remove 3,921,862 + Pf1N1B4_3514 0.67 +0.1 3,921,863 - Pf1N1B4_3514 0.67 +1.1 3,921,953 + Pf1N1B4_3514 0.72 +2.4 3,921,954 - Pf1N1B4_3514 0.72 -0.4 3,921,954 - Pf1N1B4_3514 0.72 +0.7 3,921,954 - Pf1N1B4_3514 0.72 +1.9 3,922,057 + Pf1N1B4_3514 0.77 +0.9 3,922,057 + Pf1N1B4_3514 0.77 +0.9 3,922,057 + Pf1N1B4_3514 0.77 +0.9 3,922,057 + Pf1N1B4_3514 0.77 +1.4 3,922,057 + Pf1N1B4_3514 0.77 +0.6 3,922,058 - Pf1N1B4_3514 0.77 +1.8 3,922,058 - Pf1N1B4_3514 0.77 +0.8 3,922,058 - Pf1N1B4_3514 0.77 +0.9 3,922,198 + Pf1N1B4_3514 0.84 +0.6 3,922,198 + Pf1N1B4_3514 0.84 +0.8 3,922,198 + Pf1N1B4_3514 0.84 +1.5 3,922,198 + Pf1N1B4_3514 0.84 -0.6 3,922,198 + Pf1N1B4_3514 0.84 +0.7 3,922,198 + Pf1N1B4_3514 0.84 +1.1 3,922,198 + Pf1N1B4_3514 0.84 +2.2 3,922,198 + Pf1N1B4_3514 0.84 +0.9 3,922,198 + Pf1N1B4_3514 0.84 +2.5 3,922,199 - Pf1N1B4_3514 0.84 +1.1 3,922,199 - Pf1N1B4_3514 0.84 +1.3 3,922,199 - Pf1N1B4_3514 0.84 +1.7 3,922,199 - Pf1N1B4_3514 0.84 +2.3 3,922,199 - Pf1N1B4_3514 0.84 +0.5 3,922,227 + Pf1N1B4_3514 0.86 +1.5 3,922,227 + +4.4 3,922,227 + Pf1N1B4_3514 0.86 +1.9 3,922,228 - Pf1N1B4_3514 0.86 +0.6 3,922,242 + Pf1N1B4_3514 0.87 +1.2 3,922,242 + Pf1N1B4_3514 0.87 +1.0 3,922,243 - Pf1N1B4_3514 0.87 +0.9 3,922,397 + +1.8 3,922,398 - +1.7 3,922,463 + +0.6 3,922,552 + -0.2 3,922,583 + -0.9 3,922,583 + +0.8 3,922,584 - Pf1N1B4_3515 0.10 -1.0 3,922,584 - Pf1N1B4_3515 0.10 -1.7 3,922,683 + Pf1N1B4_3515 0.21 +0.4 3,922,683 + Pf1N1B4_3515 0.21 -0.2 3,922,683 + Pf1N1B4_3515 0.21 +0.9 3,922,683 + Pf1N1B4_3515 0.21 +2.2 3,922,683 + Pf1N1B4_3515 0.21 -0.6 3,922,684 - Pf1N1B4_3515 0.21 -0.7 3,922,684 - Pf1N1B4_3515 0.21 -0.1 3,922,684 - Pf1N1B4_3515 0.21 +0.0 3,922,687 + Pf1N1B4_3515 0.21 +0.3 3,922,752 + Pf1N1B4_3515 0.28 +1.7 3,922,752 + Pf1N1B4_3515 0.28 +0.9 3,922,752 + Pf1N1B4_3515 0.28 +0.4 3,922,752 + Pf1N1B4_3515 0.28 +0.7 3,922,752 + Pf1N1B4_3515 0.28 +1.5 3,922,791 + Pf1N1B4_3515 0.32 +0.5 3,922,792 - Pf1N1B4_3515 0.32 +0.3 3,923,165 + Pf1N1B4_3515 0.72 +0.8 3,923,425 + -1.1 3,923,425 + +0.4 3,923,425 + +1.4 3,923,425 + +1.0 3,923,425 + +0.1 3,923,425 + +0.4 3,923,425 + +1.6 3,923,425 + +0.9 3,923,426 - -0.8 3,923,426 - +1.2 3,923,426 - -0.5 3,923,426 - -0.6 3,923,449 + -0.4 3,923,449 + +0.1 3,923,449 + +0.4 3,923,449 + +2.1 3,923,450 - +0.6 3,923,470 + -1.6 3,923,470 + +1.4 3,923,470 + -0.1 3,923,470 + +0.2 3,923,470 + +0.5 3,923,471 - +0.4 3,923,620 + +0.3 3,923,620 + +1.8 3,923,620 + +1.8 3,923,620 + -0.0 3,923,620 + +0.6 3,923,621 - +0.6 3,923,621 - +0.0 3,923,623 + -0.4 3,923,623 + +0.7 3,923,623 + +1.1 3,923,623 + -0.4 3,923,624 - -0.4 3,923,661 + Pf1N1B4_3516 0.11 +0.2 3,923,734 + Pf1N1B4_3516 0.15 +0.6 3,923,734 + Pf1N1B4_3516 0.15 +1.1 3,923,734 + Pf1N1B4_3516 0.15 +0.3 3,923,734 + Pf1N1B4_3516 0.15 +0.5 3,923,734 + Pf1N1B4_3516 0.15 +1.5 3,923,734 + Pf1N1B4_3516 0.15 +1.6 3,923,734 + Pf1N1B4_3516 0.15 -0.1 3,923,734 + Pf1N1B4_3516 0.15 -0.5 3,923,735 - Pf1N1B4_3516 0.15 +0.5 3,923,740 + Pf1N1B4_3516 0.15 -0.4 3,923,740 + Pf1N1B4_3516 0.15 +0.5 3,923,740 + Pf1N1B4_3516 0.15 +0.8 3,923,740 + Pf1N1B4_3516 0.15 +0.4 3,923,740 + Pf1N1B4_3516 0.15 -0.1 3,923,740 + Pf1N1B4_3516 0.15 -0.0 3,923,740 + Pf1N1B4_3516 0.15 -0.3 3,923,740 + +1.6 3,923,740 + Pf1N1B4_3516 0.15 +0.5 3,923,740 + Pf1N1B4_3516 0.15 -0.2 3,923,740 + Pf1N1B4_3516 0.15 +0.4 3,923,740 + Pf1N1B4_3516 0.15 +0.4 3,923,740 + Pf1N1B4_3516 0.15 +0.9 3,923,740 + Pf1N1B4_3516 0.15 -0.8 3,923,741 - Pf1N1B4_3516 0.15 +0.9 3,923,741 - Pf1N1B4_3516 0.15 -1.2 3,923,741 - Pf1N1B4_3516 0.15 -0.0 3,923,833 + Pf1N1B4_3516 0.19 +0.5 3,923,833 + Pf1N1B4_3516 0.19 +0.7 3,923,833 + Pf1N1B4_3516 0.19 +1.8 3,923,833 + Pf1N1B4_3516 0.19 +1.3 3,923,834 - Pf1N1B4_3516 0.19 -0.4 3,923,834 - Pf1N1B4_3516 0.19 -0.7 3,923,881 + Pf1N1B4_3516 0.22 +1.2 3,923,932 + Pf1N1B4_3516 0.24 +0.8 3,923,932 + Pf1N1B4_3516 0.24 +0.5 3,923,998 + Pf1N1B4_3516 0.27 +2.2 3,923,998 + Pf1N1B4_3516 0.27 -1.3 3,924,049 + Pf1N1B4_3516 0.29 +0.8 3,924,103 + Pf1N1B4_3516 0.32 +0.6 3,924,104 - Pf1N1B4_3516 0.32 +0.4 3,924,139 + Pf1N1B4_3516 0.34 +0.6 3,924,139 + Pf1N1B4_3516 0.34 -0.4 3,924,193 + Pf1N1B4_3516 0.36 +0.0 3,924,193 + Pf1N1B4_3516 0.36 +0.9 3,924,193 + Pf1N1B4_3516 0.36 +1.9 3,924,193 + Pf1N1B4_3516 0.36 +0.8 3,924,194 - Pf1N1B4_3516 0.36 +0.1 3,924,194 - +1.8 3,924,289 + Pf1N1B4_3516 0.41 -0.1 3,924,289 + Pf1N1B4_3516 0.41 +1.3 3,924,289 + Pf1N1B4_3516 0.41 +0.7 3,924,289 + Pf1N1B4_3516 0.41 +1.0 3,924,289 + Pf1N1B4_3516 0.41 -0.3 3,924,290 - Pf1N1B4_3516 0.41 +1.6 3,924,290 - Pf1N1B4_3516 0.41 -1.9 3,924,290 - Pf1N1B4_3516 0.41 -0.1 3,924,290 - Pf1N1B4_3516 0.41 +0.7 3,924,319 + Pf1N1B4_3516 0.42 +0.1 3,924,319 + Pf1N1B4_3516 0.42 +0.5 3,924,385 + Pf1N1B4_3516 0.45 -0.1
Or see this region's nucleotide sequence