Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3119 and Pf1N1B4_3120 are separated by 29 nucleotides Pf1N1B4_3120 and Pf1N1B4_3121 overlap by 35 nucleotides Pf1N1B4_3121 and Pf1N1B4_3122 overlap by 4 nucleotides Pf1N1B4_3122 and Pf1N1B4_3123 overlap by 1 nucleotides Pf1N1B4_3123 and Pf1N1B4_3124 are separated by 67 nucleotides Pf1N1B4_3124 and Pf1N1B4_3125 overlap by 4 nucleotides Pf1N1B4_3125 and Pf1N1B4_3126 are separated by 127 nucleotides
Pf1N1B4_3119 - Holin, at 3,507,348 to 3,507,695
_3119
Pf1N1B4_3120 - hypothetical protein, at 3,507,725 to 3,507,859
_3120
Pf1N1B4_3121 - FIG111678: IS, phage, Tn; Transposon-related functions, at 3,507,825 to 3,508,409
_3121
Pf1N1B4_3122 - Mu-like prophage FluMu protein GP38, at 3,508,406 to 3,508,588
_3122
Pf1N1B4_3123 - Bacteriophage tail sheath protein, at 3,508,588 to 3,510,084
_3123
Pf1N1B4_3124 - Phage tail tube protein, at 3,510,152 to 3,510,499
_3124
Pf1N1B4_3125 - FIG00954242: hypothetical protein, at 3,510,496 to 3,510,792
_3125
Pf1N1B4_3126 - Phage tail length tape-measure protein, at 3,510,920 to 3,512,692
_3126
Position (kb)
3508
3509
3510
3511 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3507.624 kb on + strand, within Pf1N1B4_3119 at 3507.624 kb on + strand, within Pf1N1B4_3119 at 3507.625 kb on - strand at 3507.824 kb on + strand, within Pf1N1B4_3120 at 3507.824 kb on + strand, within Pf1N1B4_3120 at 3507.824 kb on + strand, within Pf1N1B4_3120 at 3507.825 kb on - strand at 3507.913 kb on + strand, within Pf1N1B4_3121 at 3507.914 kb on - strand, within Pf1N1B4_3121 at 3507.914 kb on - strand, within Pf1N1B4_3121 at 3507.914 kb on - strand, within Pf1N1B4_3121 at 3507.948 kb on + strand, within Pf1N1B4_3121 at 3507.949 kb on - strand, within Pf1N1B4_3121 at 3507.949 kb on - strand, within Pf1N1B4_3121 at 3507.949 kb on - strand, within Pf1N1B4_3121 at 3507.949 kb on - strand, within Pf1N1B4_3121 at 3507.949 kb on - strand, within Pf1N1B4_3121 at 3508.100 kb on - strand, within Pf1N1B4_3121 at 3508.100 kb on - strand, within Pf1N1B4_3121 at 3508.137 kb on + strand, within Pf1N1B4_3121 at 3508.152 kb on + strand, within Pf1N1B4_3121 at 3508.153 kb on - strand, within Pf1N1B4_3121 at 3508.399 kb on + strand at 3508.399 kb on + strand at 3508.587 kb on - strand at 3508.639 kb on + strand at 3508.639 kb on + strand at 3508.639 kb on + strand at 3508.640 kb on - strand at 3508.640 kb on - strand at 3508.813 kb on + strand, within Pf1N1B4_3123 at 3508.813 kb on + strand, within Pf1N1B4_3123 at 3508.813 kb on + strand at 3508.813 kb on + strand, within Pf1N1B4_3123 at 3508.813 kb on + strand, within Pf1N1B4_3123 at 3508.813 kb on + strand, within Pf1N1B4_3123 at 3508.814 kb on - strand, within Pf1N1B4_3123 at 3508.814 kb on - strand, within Pf1N1B4_3123 at 3508.945 kb on + strand, within Pf1N1B4_3123 at 3508.945 kb on + strand at 3508.945 kb on + strand, within Pf1N1B4_3123 at 3509.374 kb on + strand, within Pf1N1B4_3123 at 3509.374 kb on + strand, within Pf1N1B4_3123 at 3509.374 kb on + strand, within Pf1N1B4_3123 at 3509.633 kb on - strand, within Pf1N1B4_3123 at 3509.633 kb on - strand, within Pf1N1B4_3123 at 3509.650 kb on + strand, within Pf1N1B4_3123 at 3509.650 kb on + strand, within Pf1N1B4_3123 at 3509.651 kb on - strand, within Pf1N1B4_3123 at 3509.651 kb on - strand, within Pf1N1B4_3123 at 3509.653 kb on + strand, within Pf1N1B4_3123 at 3509.653 kb on + strand, within Pf1N1B4_3123 at 3509.666 kb on - strand, within Pf1N1B4_3123 at 3509.666 kb on - strand, within Pf1N1B4_3123 at 3509.695 kb on + strand, within Pf1N1B4_3123 at 3509.695 kb on + strand, within Pf1N1B4_3123 at 3509.695 kb on + strand, within Pf1N1B4_3123 at 3509.696 kb on - strand, within Pf1N1B4_3123 at 3509.696 kb on - strand, within Pf1N1B4_3123 at 3509.731 kb on + strand, within Pf1N1B4_3123 at 3509.732 kb on - strand, within Pf1N1B4_3123 at 3509.732 kb on - strand, within Pf1N1B4_3123 at 3509.799 kb on + strand, within Pf1N1B4_3123 at 3509.803 kb on + strand, within Pf1N1B4_3123 at 3509.803 kb on + strand, within Pf1N1B4_3123 at 3509.803 kb on + strand, within Pf1N1B4_3123 at 3509.804 kb on - strand, within Pf1N1B4_3123 at 3509.804 kb on - strand, within Pf1N1B4_3123 at 3510.016 kb on + strand at 3510.016 kb on + strand at 3510.016 kb on + strand at 3510.017 kb on - strand at 3510.017 kb on - strand at 3510.017 kb on - strand at 3510.017 kb on - strand at 3510.017 kb on - strand at 3510.067 kb on + strand at 3510.067 kb on + strand at 3510.067 kb on + strand at 3510.067 kb on + strand at 3510.068 kb on - strand at 3510.132 kb on + strand at 3510.132 kb on + strand at 3510.133 kb on - strand at 3510.133 kb on - strand at 3510.179 kb on + strand at 3510.179 kb on + strand at 3510.179 kb on + strand at 3510.180 kb on - strand at 3510.209 kb on - strand, within Pf1N1B4_3124 at 3510.272 kb on + strand, within Pf1N1B4_3124 at 3510.272 kb on + strand, within Pf1N1B4_3124 at 3510.272 kb on + strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.398 kb on + strand, within Pf1N1B4_3124 at 3510.416 kb on + strand, within Pf1N1B4_3124 at 3510.611 kb on - strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.665 kb on - strand, within Pf1N1B4_3125 at 3510.665 kb on - strand, within Pf1N1B4_3125 at 3510.665 kb on - strand, within Pf1N1B4_3125 at 3510.805 kb on - strand at 3510.805 kb on - strand at 3510.805 kb on - strand at 3511.063 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 remove 3,507,624 + Pf1N1B4_3119 0.79 +0.0 3,507,624 + Pf1N1B4_3119 0.79 -0.7 3,507,625 - +1.6 3,507,824 + Pf1N1B4_3120 0.73 -0.0 3,507,824 + Pf1N1B4_3120 0.73 -1.4 3,507,824 + Pf1N1B4_3120 0.73 -0.6 3,507,825 - +0.4 3,507,913 + Pf1N1B4_3121 0.15 -1.1 3,507,914 - Pf1N1B4_3121 0.15 +1.2 3,507,914 - Pf1N1B4_3121 0.15 -0.8 3,507,914 - Pf1N1B4_3121 0.15 -0.8 3,507,948 + Pf1N1B4_3121 0.21 -1.2 3,507,949 - Pf1N1B4_3121 0.21 +0.6 3,507,949 - Pf1N1B4_3121 0.21 -1.4 3,507,949 - Pf1N1B4_3121 0.21 +1.6 3,507,949 - Pf1N1B4_3121 0.21 -0.0 3,507,949 - Pf1N1B4_3121 0.21 -0.6 3,508,100 - Pf1N1B4_3121 0.47 -0.7 3,508,100 - Pf1N1B4_3121 0.47 +0.7 3,508,137 + Pf1N1B4_3121 0.53 +0.8 3,508,152 + Pf1N1B4_3121 0.56 -1.6 3,508,153 - Pf1N1B4_3121 0.56 -0.2 3,508,399 + +0.3 3,508,399 + -0.0 3,508,587 - -0.5 3,508,639 + -1.2 3,508,639 + -0.1 3,508,639 + -1.2 3,508,640 - -0.4 3,508,640 - +0.1 3,508,813 + Pf1N1B4_3123 0.15 +0.4 3,508,813 + Pf1N1B4_3123 0.15 +0.2 3,508,813 + +0.3 3,508,813 + Pf1N1B4_3123 0.15 +1.0 3,508,813 + Pf1N1B4_3123 0.15 -2.1 3,508,813 + Pf1N1B4_3123 0.15 +0.5 3,508,814 - Pf1N1B4_3123 0.15 -0.3 3,508,814 - Pf1N1B4_3123 0.15 -1.0 3,508,945 + Pf1N1B4_3123 0.24 -1.5 3,508,945 + +0.2 3,508,945 + Pf1N1B4_3123 0.24 -0.2 3,509,374 + Pf1N1B4_3123 0.53 +1.2 3,509,374 + Pf1N1B4_3123 0.53 +0.2 3,509,374 + Pf1N1B4_3123 0.53 +1.8 3,509,633 - Pf1N1B4_3123 0.70 -1.1 3,509,633 - Pf1N1B4_3123 0.70 -0.4 3,509,650 + Pf1N1B4_3123 0.71 -0.0 3,509,650 + Pf1N1B4_3123 0.71 -1.0 3,509,651 - Pf1N1B4_3123 0.71 +1.0 3,509,651 - Pf1N1B4_3123 0.71 +0.1 3,509,653 + Pf1N1B4_3123 0.71 -0.0 3,509,653 + Pf1N1B4_3123 0.71 +0.6 3,509,666 - Pf1N1B4_3123 0.72 +0.2 3,509,666 - Pf1N1B4_3123 0.72 -1.1 3,509,695 + Pf1N1B4_3123 0.74 +0.6 3,509,695 + Pf1N1B4_3123 0.74 -0.2 3,509,695 + Pf1N1B4_3123 0.74 +1.6 3,509,696 - Pf1N1B4_3123 0.74 -0.5 3,509,696 - Pf1N1B4_3123 0.74 +0.5 3,509,731 + Pf1N1B4_3123 0.76 +0.2 3,509,732 - Pf1N1B4_3123 0.76 -2.3 3,509,732 - Pf1N1B4_3123 0.76 +1.2 3,509,799 + Pf1N1B4_3123 0.81 +0.2 3,509,803 + Pf1N1B4_3123 0.81 +1.0 3,509,803 + Pf1N1B4_3123 0.81 -2.2 3,509,803 + Pf1N1B4_3123 0.81 -0.2 3,509,804 - Pf1N1B4_3123 0.81 +0.5 3,509,804 - Pf1N1B4_3123 0.81 -2.6 3,510,016 + +0.6 3,510,016 + -1.6 3,510,016 + -1.2 3,510,017 - -0.3 3,510,017 - +0.1 3,510,017 - -0.6 3,510,017 - -1.0 3,510,017 - -1.2 3,510,067 + +0.6 3,510,067 + -1.3 3,510,067 + +0.6 3,510,067 + -0.3 3,510,068 - -0.2 3,510,132 + +1.0 3,510,132 + -0.0 3,510,133 - +0.4 3,510,133 - -0.4 3,510,179 + -0.2 3,510,179 + -0.1 3,510,179 + -0.8 3,510,180 - -0.5 3,510,209 - Pf1N1B4_3124 0.16 +0.1 3,510,272 + Pf1N1B4_3124 0.34 +3.6 3,510,272 + Pf1N1B4_3124 0.34 +0.7 3,510,272 + Pf1N1B4_3124 0.34 +0.4 3,510,273 - Pf1N1B4_3124 0.35 -2.3 3,510,273 - Pf1N1B4_3124 0.35 +1.7 3,510,273 - Pf1N1B4_3124 0.35 +0.4 3,510,273 - Pf1N1B4_3124 0.35 -0.5 3,510,273 - Pf1N1B4_3124 0.35 +0.6 3,510,273 - Pf1N1B4_3124 0.35 +0.8 3,510,273 - Pf1N1B4_3124 0.35 +0.7 3,510,398 + Pf1N1B4_3124 0.71 -1.2 3,510,416 + Pf1N1B4_3124 0.76 -1.1 3,510,611 - Pf1N1B4_3125 0.39 -0.2 3,510,664 + Pf1N1B4_3125 0.57 +0.6 3,510,664 + Pf1N1B4_3125 0.57 +0.3 3,510,664 + Pf1N1B4_3125 0.57 -0.0 3,510,664 + Pf1N1B4_3125 0.57 -0.8 3,510,664 + Pf1N1B4_3125 0.57 -1.0 3,510,664 + Pf1N1B4_3125 0.57 -1.4 3,510,665 - Pf1N1B4_3125 0.57 +2.0 3,510,665 - Pf1N1B4_3125 0.57 +0.8 3,510,665 - Pf1N1B4_3125 0.57 -0.3 3,510,805 - +1.6 3,510,805 - +1.9 3,510,805 - -0.8 3,511,063 + +0.2
Or see this region's nucleotide sequence