Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1988 and Pf1N1B4_1989 overlap by 1 nucleotides Pf1N1B4_1989 and Pf1N1B4_1990 overlap by 4 nucleotides Pf1N1B4_1990 and Pf1N1B4_1991 are separated by 86 nucleotides
Pf1N1B4_1988 - Zinc ABC transporter, inner membrane permease protein ZnuB, at 2,273,679 to 2,274,545
_1988
Pf1N1B4_1989 - Zinc ABC transporter, ATP-binding protein ZnuC, at 2,274,545 to 2,275,213
_1989
Pf1N1B4_1990 - GTP cyclohydrolase I (EC 3.5.4.16) type 2, at 2,275,210 to 2,276,106
_1990
Pf1N1B4_1991 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 2,276,193 to 2,277,401
_1991
Position (kb)
2275
2276
2277 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2274.426 kb on + strand, within Pf1N1B4_1988 at 2274.427 kb on - strand, within Pf1N1B4_1988 at 2274.624 kb on - strand, within Pf1N1B4_1989 at 2275.485 kb on - strand, within Pf1N1B4_1990 at 2275.485 kb on - strand, within Pf1N1B4_1990 at 2275.673 kb on + strand, within Pf1N1B4_1990 at 2275.674 kb on - strand, within Pf1N1B4_1990 at 2275.792 kb on + strand, within Pf1N1B4_1990 at 2275.792 kb on + strand at 2275.792 kb on + strand, within Pf1N1B4_1990 at 2275.793 kb on - strand, within Pf1N1B4_1990 at 2275.793 kb on - strand, within Pf1N1B4_1990 at 2275.793 kb on - strand, within Pf1N1B4_1990 at 2275.793 kb on - strand, within Pf1N1B4_1990 at 2275.793 kb on - strand, within Pf1N1B4_1990 at 2275.815 kb on - strand, within Pf1N1B4_1990 at 2275.919 kb on + strand, within Pf1N1B4_1990 at 2275.919 kb on + strand, within Pf1N1B4_1990 at 2275.920 kb on - strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.933 kb on - strand, within Pf1N1B4_1990 at 2275.933 kb on - strand, within Pf1N1B4_1990 at 2275.933 kb on - strand, within Pf1N1B4_1990 at 2275.933 kb on - strand, within Pf1N1B4_1990 at 2276.343 kb on + strand, within Pf1N1B4_1991 at 2276.343 kb on + strand, within Pf1N1B4_1991 at 2276.343 kb on + strand, within Pf1N1B4_1991 at 2276.344 kb on - strand, within Pf1N1B4_1991 at 2276.344 kb on - strand, within Pf1N1B4_1991 at 2276.344 kb on - strand, within Pf1N1B4_1991 at 2276.344 kb on - strand, within Pf1N1B4_1991 at 2276.344 kb on - strand, within Pf1N1B4_1991 at 2276.344 kb on - strand, within Pf1N1B4_1991 at 2276.344 kb on - strand, within Pf1N1B4_1991 at 2276.426 kb on - strand, within Pf1N1B4_1991 at 2276.438 kb on + strand, within Pf1N1B4_1991 at 2276.439 kb on - strand, within Pf1N1B4_1991 at 2276.941 kb on - strand, within Pf1N1B4_1991
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 remove 2,274,426 + Pf1N1B4_1988 0.86 +0.2 2,274,427 - Pf1N1B4_1988 0.86 +1.0 2,274,624 - Pf1N1B4_1989 0.12 -0.7 2,275,485 - Pf1N1B4_1990 0.31 +1.7 2,275,485 - Pf1N1B4_1990 0.31 -1.1 2,275,673 + Pf1N1B4_1990 0.52 -0.1 2,275,674 - Pf1N1B4_1990 0.52 +0.2 2,275,792 + Pf1N1B4_1990 0.65 -0.2 2,275,792 + +0.0 2,275,792 + Pf1N1B4_1990 0.65 +0.6 2,275,793 - Pf1N1B4_1990 0.65 -0.4 2,275,793 - Pf1N1B4_1990 0.65 +0.6 2,275,793 - Pf1N1B4_1990 0.65 -1.9 2,275,793 - Pf1N1B4_1990 0.65 -0.6 2,275,793 - Pf1N1B4_1990 0.65 -1.0 2,275,815 - Pf1N1B4_1990 0.67 -1.2 2,275,919 + Pf1N1B4_1990 0.79 +0.5 2,275,919 + Pf1N1B4_1990 0.79 +0.2 2,275,920 - Pf1N1B4_1990 0.79 -0.4 2,275,932 + Pf1N1B4_1990 0.80 +1.5 2,275,932 + Pf1N1B4_1990 0.80 -1.2 2,275,932 + Pf1N1B4_1990 0.80 +0.4 2,275,932 + Pf1N1B4_1990 0.80 -1.0 2,275,932 + Pf1N1B4_1990 0.80 +0.6 2,275,932 + Pf1N1B4_1990 0.80 -1.2 2,275,932 + Pf1N1B4_1990 0.80 +0.3 2,275,932 + Pf1N1B4_1990 0.80 +0.2 2,275,932 + Pf1N1B4_1990 0.80 +0.9 2,275,932 + Pf1N1B4_1990 0.80 -1.0 2,275,932 + Pf1N1B4_1990 0.80 +0.6 2,275,932 + Pf1N1B4_1990 0.80 +0.6 2,275,932 + Pf1N1B4_1990 0.80 +2.3 2,275,932 + Pf1N1B4_1990 0.80 -0.7 2,275,932 + Pf1N1B4_1990 0.80 +1.6 2,275,933 - Pf1N1B4_1990 0.81 +0.7 2,275,933 - Pf1N1B4_1990 0.81 +1.0 2,275,933 - Pf1N1B4_1990 0.81 -0.2 2,275,933 - Pf1N1B4_1990 0.81 +1.3 2,276,343 + Pf1N1B4_1991 0.12 -0.6 2,276,343 + Pf1N1B4_1991 0.12 -0.1 2,276,343 + Pf1N1B4_1991 0.12 +1.6 2,276,344 - Pf1N1B4_1991 0.12 +1.0 2,276,344 - Pf1N1B4_1991 0.12 -0.4 2,276,344 - Pf1N1B4_1991 0.12 +0.1 2,276,344 - Pf1N1B4_1991 0.12 +1.0 2,276,344 - Pf1N1B4_1991 0.12 -0.9 2,276,344 - Pf1N1B4_1991 0.12 -0.2 2,276,344 - Pf1N1B4_1991 0.12 -1.3 2,276,426 - Pf1N1B4_1991 0.19 -1.2 2,276,438 + Pf1N1B4_1991 0.20 -0.6 2,276,439 - Pf1N1B4_1991 0.20 +0.3 2,276,941 - Pf1N1B4_1991 0.62 +1.6
Or see this region's nucleotide sequence