Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1990

Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1988 and Pf1N1B4_1989 overlap by 1 nucleotidesPf1N1B4_1989 and Pf1N1B4_1990 overlap by 4 nucleotidesPf1N1B4_1990 and Pf1N1B4_1991 are separated by 86 nucleotides Pf1N1B4_1988 - Zinc ABC transporter, inner membrane permease protein ZnuB, at 2,273,679 to 2,274,545 _1988 Pf1N1B4_1989 - Zinc ABC transporter, ATP-binding protein ZnuC, at 2,274,545 to 2,275,213 _1989 Pf1N1B4_1990 - GTP cyclohydrolase I (EC 3.5.4.16) type 2, at 2,275,210 to 2,276,106 _1990 Pf1N1B4_1991 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 2,276,193 to 2,277,401 _1991 Position (kb) 2275 2276 2277Strain fitness (log2 ratio) -2 -1 0 1 2at 2274.426 kb on + strand, within Pf1N1B4_1988at 2274.427 kb on - strand, within Pf1N1B4_1988at 2274.624 kb on - strand, within Pf1N1B4_1989at 2275.485 kb on - strand, within Pf1N1B4_1990at 2275.485 kb on - strand, within Pf1N1B4_1990at 2275.673 kb on + strand, within Pf1N1B4_1990at 2275.674 kb on - strand, within Pf1N1B4_1990at 2275.792 kb on + strand, within Pf1N1B4_1990at 2275.792 kb on + strandat 2275.792 kb on + strand, within Pf1N1B4_1990at 2275.793 kb on - strand, within Pf1N1B4_1990at 2275.793 kb on - strand, within Pf1N1B4_1990at 2275.793 kb on - strand, within Pf1N1B4_1990at 2275.793 kb on - strand, within Pf1N1B4_1990at 2275.793 kb on - strand, within Pf1N1B4_1990at 2275.815 kb on - strand, within Pf1N1B4_1990at 2275.919 kb on + strand, within Pf1N1B4_1990at 2275.919 kb on + strand, within Pf1N1B4_1990at 2275.920 kb on - strand, within Pf1N1B4_1990at 2275.932 kb on + strand, within Pf1N1B4_1990at 2275.932 kb on + strand, within Pf1N1B4_1990at 2275.932 kb on + strand, within Pf1N1B4_1990at 2275.932 kb on + strand, within Pf1N1B4_1990at 2275.932 kb on + strand, within Pf1N1B4_1990at 2275.932 kb on + strand, within Pf1N1B4_1990at 2275.932 kb on + strand, within Pf1N1B4_1990at 2275.932 kb on + strand, within Pf1N1B4_1990at 2275.932 kb on + strand, within Pf1N1B4_1990at 2275.932 kb on + strand, within Pf1N1B4_1990at 2275.932 kb on + strand, within Pf1N1B4_1990at 2275.932 kb on + strand, within Pf1N1B4_1990at 2275.932 kb on + strand, within Pf1N1B4_1990at 2275.932 kb on + strand, within Pf1N1B4_1990at 2275.932 kb on + strand, within Pf1N1B4_1990at 2275.933 kb on - strand, within Pf1N1B4_1990at 2275.933 kb on - strand, within Pf1N1B4_1990at 2275.933 kb on - strand, within Pf1N1B4_1990at 2275.933 kb on - strand, within Pf1N1B4_1990at 2276.343 kb on + strand, within Pf1N1B4_1991at 2276.343 kb on + strand, within Pf1N1B4_1991at 2276.343 kb on + strand, within Pf1N1B4_1991at 2276.344 kb on - strand, within Pf1N1B4_1991at 2276.344 kb on - strand, within Pf1N1B4_1991at 2276.344 kb on - strand, within Pf1N1B4_1991at 2276.344 kb on - strand, within Pf1N1B4_1991at 2276.344 kb on - strand, within Pf1N1B4_1991at 2276.344 kb on - strand, within Pf1N1B4_1991at 2276.344 kb on - strand, within Pf1N1B4_1991at 2276.426 kb on - strand, within Pf1N1B4_1991at 2276.438 kb on + strand, within Pf1N1B4_1991at 2276.439 kb on - strand, within Pf1N1B4_1991at 2276.941 kb on - strand, within Pf1N1B4_1991

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Per-strain Table

Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1
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2,274,426 + Pf1N1B4_1988 0.86 +0.2
2,274,427 - Pf1N1B4_1988 0.86 +1.0
2,274,624 - Pf1N1B4_1989 0.12 -0.7
2,275,485 - Pf1N1B4_1990 0.31 +1.7
2,275,485 - Pf1N1B4_1990 0.31 -1.1
2,275,673 + Pf1N1B4_1990 0.52 -0.1
2,275,674 - Pf1N1B4_1990 0.52 +0.2
2,275,792 + Pf1N1B4_1990 0.65 -0.2
2,275,792 + +0.0
2,275,792 + Pf1N1B4_1990 0.65 +0.6
2,275,793 - Pf1N1B4_1990 0.65 -0.4
2,275,793 - Pf1N1B4_1990 0.65 +0.6
2,275,793 - Pf1N1B4_1990 0.65 -1.9
2,275,793 - Pf1N1B4_1990 0.65 -0.6
2,275,793 - Pf1N1B4_1990 0.65 -1.0
2,275,815 - Pf1N1B4_1990 0.67 -1.2
2,275,919 + Pf1N1B4_1990 0.79 +0.5
2,275,919 + Pf1N1B4_1990 0.79 +0.2
2,275,920 - Pf1N1B4_1990 0.79 -0.4
2,275,932 + Pf1N1B4_1990 0.80 +1.5
2,275,932 + Pf1N1B4_1990 0.80 -1.2
2,275,932 + Pf1N1B4_1990 0.80 +0.4
2,275,932 + Pf1N1B4_1990 0.80 -1.0
2,275,932 + Pf1N1B4_1990 0.80 +0.6
2,275,932 + Pf1N1B4_1990 0.80 -1.2
2,275,932 + Pf1N1B4_1990 0.80 +0.3
2,275,932 + Pf1N1B4_1990 0.80 +0.2
2,275,932 + Pf1N1B4_1990 0.80 +0.9
2,275,932 + Pf1N1B4_1990 0.80 -1.0
2,275,932 + Pf1N1B4_1990 0.80 +0.6
2,275,932 + Pf1N1B4_1990 0.80 +0.6
2,275,932 + Pf1N1B4_1990 0.80 +2.3
2,275,932 + Pf1N1B4_1990 0.80 -0.7
2,275,932 + Pf1N1B4_1990 0.80 +1.6
2,275,933 - Pf1N1B4_1990 0.81 +0.7
2,275,933 - Pf1N1B4_1990 0.81 +1.0
2,275,933 - Pf1N1B4_1990 0.81 -0.2
2,275,933 - Pf1N1B4_1990 0.81 +1.3
2,276,343 + Pf1N1B4_1991 0.12 -0.6
2,276,343 + Pf1N1B4_1991 0.12 -0.1
2,276,343 + Pf1N1B4_1991 0.12 +1.6
2,276,344 - Pf1N1B4_1991 0.12 +1.0
2,276,344 - Pf1N1B4_1991 0.12 -0.4
2,276,344 - Pf1N1B4_1991 0.12 +0.1
2,276,344 - Pf1N1B4_1991 0.12 +1.0
2,276,344 - Pf1N1B4_1991 0.12 -0.9
2,276,344 - Pf1N1B4_1991 0.12 -0.2
2,276,344 - Pf1N1B4_1991 0.12 -1.3
2,276,426 - Pf1N1B4_1991 0.19 -1.2
2,276,438 + Pf1N1B4_1991 0.20 -0.6
2,276,439 - Pf1N1B4_1991 0.20 +0.3
2,276,941 - Pf1N1B4_1991 0.62 +1.6

Or see this region's nucleotide sequence