Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1372 and Pf1N1B4_1373 are separated by 37 nucleotides Pf1N1B4_1373 and Pf1N1B4_1374 are separated by 28 nucleotides Pf1N1B4_1374 and Pf1N1B4_1375 are separated by 201 nucleotides
Pf1N1B4_1372 - Urease accessory protein UreF, at 1,570,656 to 1,571,330
_1372
Pf1N1B4_1373 - Urease accessory protein UreG, at 1,571,368 to 1,571,982
_1373
Pf1N1B4_1374 - HupE-UreJ family metal transporter, at 1,572,011 to 1,572,583
_1374
Pf1N1B4_1375 - Mannose-6-phosphate isomerase (EC 5.3.1.8), at 1,572,785 to 1,573,912
_1375
Position (kb)
1572
1573 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1571.307 kb on + strand at 1571.307 kb on + strand at 1571.308 kb on - strand at 1571.308 kb on - strand at 1571.328 kb on + strand at 1571.338 kb on + strand at 1571.339 kb on - strand at 1571.352 kb on + strand at 1571.352 kb on + strand at 1571.353 kb on - strand at 1571.494 kb on + strand, within Pf1N1B4_1373 at 1571.494 kb on + strand, within Pf1N1B4_1373 at 1571.494 kb on + strand, within Pf1N1B4_1373 at 1571.494 kb on + strand, within Pf1N1B4_1373 at 1571.494 kb on + strand, within Pf1N1B4_1373 at 1571.495 kb on - strand, within Pf1N1B4_1373 at 1571.495 kb on - strand, within Pf1N1B4_1373 at 1571.495 kb on - strand, within Pf1N1B4_1373 at 1571.495 kb on - strand, within Pf1N1B4_1373 at 1571.495 kb on - strand, within Pf1N1B4_1373 at 1571.495 kb on - strand, within Pf1N1B4_1373 at 1571.495 kb on - strand, within Pf1N1B4_1373 at 1571.495 kb on - strand, within Pf1N1B4_1373 at 1571.731 kb on + strand, within Pf1N1B4_1373 at 1571.731 kb on + strand, within Pf1N1B4_1373 at 1571.731 kb on + strand, within Pf1N1B4_1373 at 1571.731 kb on + strand, within Pf1N1B4_1373 at 1571.731 kb on + strand, within Pf1N1B4_1373 at 1571.731 kb on + strand, within Pf1N1B4_1373 at 1571.732 kb on - strand, within Pf1N1B4_1373 at 1571.732 kb on - strand, within Pf1N1B4_1373 at 1571.732 kb on - strand, within Pf1N1B4_1373 at 1571.732 kb on - strand, within Pf1N1B4_1373 at 1571.837 kb on + strand, within Pf1N1B4_1373 at 1571.850 kb on - strand, within Pf1N1B4_1373 at 1571.990 kb on + strand at 1571.990 kb on + strand at 1571.990 kb on + strand at 1571.990 kb on + strand at 1571.991 kb on - strand at 1571.991 kb on - strand at 1571.991 kb on - strand at 1571.991 kb on - strand at 1571.991 kb on - strand at 1571.991 kb on - strand at 1571.991 kb on - strand at 1571.991 kb on - strand at 1572.007 kb on - strand at 1572.007 kb on - strand at 1572.226 kb on + strand, within Pf1N1B4_1374 at 1572.227 kb on - strand, within Pf1N1B4_1374 at 1572.437 kb on + strand, within Pf1N1B4_1374 at 1572.437 kb on + strand, within Pf1N1B4_1374 at 1572.437 kb on + strand, within Pf1N1B4_1374 at 1572.437 kb on + strand, within Pf1N1B4_1374 at 1572.635 kb on + strand at 1572.635 kb on + strand at 1572.743 kb on + strand at 1572.992 kb on + strand, within Pf1N1B4_1375 at 1572.992 kb on + strand, within Pf1N1B4_1375 at 1572.992 kb on + strand, within Pf1N1B4_1375 at 1572.992 kb on + strand, within Pf1N1B4_1375 at 1572.992 kb on + strand, within Pf1N1B4_1375 at 1572.993 kb on - strand, within Pf1N1B4_1375 at 1572.993 kb on - strand, within Pf1N1B4_1375 at 1573.103 kb on + strand, within Pf1N1B4_1375 at 1573.103 kb on + strand, within Pf1N1B4_1375 at 1573.104 kb on - strand, within Pf1N1B4_1375 at 1573.151 kb on + strand, within Pf1N1B4_1375 at 1573.151 kb on + strand, within Pf1N1B4_1375 at 1573.152 kb on - strand, within Pf1N1B4_1375 at 1573.280 kb on + strand, within Pf1N1B4_1375 at 1573.280 kb on + strand, within Pf1N1B4_1375 at 1573.280 kb on + strand, within Pf1N1B4_1375 at 1573.280 kb on + strand, within Pf1N1B4_1375 at 1573.281 kb on - strand, within Pf1N1B4_1375 at 1573.281 kb on - strand, within Pf1N1B4_1375 at 1573.281 kb on - strand, within Pf1N1B4_1375 at 1573.281 kb on - strand, within Pf1N1B4_1375 at 1573.392 kb on + strand, within Pf1N1B4_1375 at 1573.392 kb on + strand, within Pf1N1B4_1375 at 1573.392 kb on + strand, within Pf1N1B4_1375 at 1573.392 kb on + strand, within Pf1N1B4_1375 at 1573.392 kb on + strand, within Pf1N1B4_1375 at 1573.393 kb on - strand, within Pf1N1B4_1375 at 1573.393 kb on - strand, within Pf1N1B4_1375 at 1573.393 kb on - strand, within Pf1N1B4_1375 at 1573.393 kb on - strand, within Pf1N1B4_1375 at 1573.393 kb on - strand, within Pf1N1B4_1375 at 1573.393 kb on - strand, within Pf1N1B4_1375 at 1573.419 kb on + strand, within Pf1N1B4_1375 at 1573.420 kb on - strand, within Pf1N1B4_1375 at 1573.495 kb on + strand, within Pf1N1B4_1375 at 1573.529 kb on + strand, within Pf1N1B4_1375 at 1573.529 kb on + strand, within Pf1N1B4_1375 at 1573.529 kb on + strand, within Pf1N1B4_1375 at 1573.530 kb on - strand, within Pf1N1B4_1375 at 1573.530 kb on - strand, within Pf1N1B4_1375 at 1573.530 kb on - strand, within Pf1N1B4_1375 at 1573.530 kb on - strand, within Pf1N1B4_1375 at 1573.530 kb on - strand, within Pf1N1B4_1375
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 remove 1,571,307 + +0.6 1,571,307 + +0.1 1,571,308 - +1.1 1,571,308 - -0.4 1,571,328 + +0.8 1,571,338 + -2.0 1,571,339 - -1.6 1,571,352 + -1.1 1,571,352 + +0.8 1,571,353 - -1.0 1,571,494 + Pf1N1B4_1373 0.20 +0.2 1,571,494 + Pf1N1B4_1373 0.20 +0.2 1,571,494 + Pf1N1B4_1373 0.20 +0.3 1,571,494 + Pf1N1B4_1373 0.20 -0.0 1,571,494 + Pf1N1B4_1373 0.20 -0.1 1,571,495 - Pf1N1B4_1373 0.21 -0.0 1,571,495 - Pf1N1B4_1373 0.21 -1.4 1,571,495 - Pf1N1B4_1373 0.21 -0.7 1,571,495 - Pf1N1B4_1373 0.21 -0.8 1,571,495 - Pf1N1B4_1373 0.21 -0.4 1,571,495 - Pf1N1B4_1373 0.21 -2.2 1,571,495 - Pf1N1B4_1373 0.21 -0.8 1,571,495 - Pf1N1B4_1373 0.21 -1.0 1,571,731 + Pf1N1B4_1373 0.59 -1.3 1,571,731 + Pf1N1B4_1373 0.59 +0.3 1,571,731 + Pf1N1B4_1373 0.59 -1.2 1,571,731 + Pf1N1B4_1373 0.59 +0.8 1,571,731 + Pf1N1B4_1373 0.59 -0.9 1,571,731 + Pf1N1B4_1373 0.59 -0.8 1,571,732 - Pf1N1B4_1373 0.59 +0.3 1,571,732 - Pf1N1B4_1373 0.59 -1.8 1,571,732 - Pf1N1B4_1373 0.59 -0.9 1,571,732 - Pf1N1B4_1373 0.59 -0.8 1,571,837 + Pf1N1B4_1373 0.76 -2.1 1,571,850 - Pf1N1B4_1373 0.78 -1.6 1,571,990 + +0.1 1,571,990 + +0.4 1,571,990 + -0.2 1,571,990 + +0.1 1,571,991 - -0.1 1,571,991 - -0.2 1,571,991 - -0.7 1,571,991 - +0.2 1,571,991 - -1.0 1,571,991 - -0.2 1,571,991 - -0.8 1,571,991 - -0.8 1,572,007 - -1.1 1,572,007 - -0.5 1,572,226 + Pf1N1B4_1374 0.38 -0.8 1,572,227 - Pf1N1B4_1374 0.38 -0.0 1,572,437 + Pf1N1B4_1374 0.74 +1.6 1,572,437 + Pf1N1B4_1374 0.74 -1.4 1,572,437 + Pf1N1B4_1374 0.74 -1.5 1,572,437 + Pf1N1B4_1374 0.74 -1.2 1,572,635 + +0.2 1,572,635 + +1.3 1,572,743 + -0.0 1,572,992 + Pf1N1B4_1375 0.18 -0.6 1,572,992 + Pf1N1B4_1375 0.18 +0.8 1,572,992 + Pf1N1B4_1375 0.18 -1.5 1,572,992 + Pf1N1B4_1375 0.18 +0.2 1,572,992 + Pf1N1B4_1375 0.18 -0.7 1,572,993 - Pf1N1B4_1375 0.18 -1.6 1,572,993 - Pf1N1B4_1375 0.18 +1.0 1,573,103 + Pf1N1B4_1375 0.28 -0.5 1,573,103 + Pf1N1B4_1375 0.28 -1.2 1,573,104 - Pf1N1B4_1375 0.28 -0.6 1,573,151 + Pf1N1B4_1375 0.32 -0.6 1,573,151 + Pf1N1B4_1375 0.32 -1.0 1,573,152 - Pf1N1B4_1375 0.33 -0.2 1,573,280 + Pf1N1B4_1375 0.44 -0.5 1,573,280 + Pf1N1B4_1375 0.44 -0.3 1,573,280 + Pf1N1B4_1375 0.44 -0.2 1,573,280 + Pf1N1B4_1375 0.44 -1.2 1,573,281 - Pf1N1B4_1375 0.44 -0.0 1,573,281 - Pf1N1B4_1375 0.44 -1.4 1,573,281 - Pf1N1B4_1375 0.44 -2.0 1,573,281 - Pf1N1B4_1375 0.44 -0.0 1,573,392 + Pf1N1B4_1375 0.54 -0.0 1,573,392 + Pf1N1B4_1375 0.54 -0.2 1,573,392 + Pf1N1B4_1375 0.54 -2.7 1,573,392 + Pf1N1B4_1375 0.54 +0.0 1,573,392 + Pf1N1B4_1375 0.54 +0.1 1,573,393 - Pf1N1B4_1375 0.54 +0.4 1,573,393 - Pf1N1B4_1375 0.54 +1.3 1,573,393 - Pf1N1B4_1375 0.54 -2.1 1,573,393 - Pf1N1B4_1375 0.54 -1.7 1,573,393 - Pf1N1B4_1375 0.54 -1.6 1,573,393 - Pf1N1B4_1375 0.54 -0.4 1,573,419 + Pf1N1B4_1375 0.56 -0.0 1,573,420 - Pf1N1B4_1375 0.56 +0.8 1,573,495 + Pf1N1B4_1375 0.63 +1.0 1,573,529 + Pf1N1B4_1375 0.66 +1.0 1,573,529 + Pf1N1B4_1375 0.66 -1.8 1,573,529 + Pf1N1B4_1375 0.66 -0.8 1,573,530 - Pf1N1B4_1375 0.66 -0.3 1,573,530 - Pf1N1B4_1375 0.66 -0.6 1,573,530 - Pf1N1B4_1375 0.66 -1.6 1,573,530 - Pf1N1B4_1375 0.66 -0.1 1,573,530 - Pf1N1B4_1375 0.66 +3.6
Or see this region's nucleotide sequence