Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_905 and Pf1N1B4_906 overlap by 1 nucleotides Pf1N1B4_906 and Pf1N1B4_907 are separated by 123 nucleotides
Pf1N1B4_905 - Nucleoid-associated protein, at 1,032,776 to 1,033,780
_905
Pf1N1B4_906 - Glutaredoxin NrdH, at 1,033,780 to 1,034,130
_906
Pf1N1B4_907 - Probable glutathione s-transferase protein (EC 2.5.1.18), at 1,034,254 to 1,035,189
_907
Position (kb)
1033
1034
1035 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1032.884 kb on + strand at 1032.884 kb on + strand, within Pf1N1B4_905 at 1032.884 kb on + strand, within Pf1N1B4_905 at 1032.884 kb on + strand, within Pf1N1B4_905 at 1032.884 kb on + strand at 1032.884 kb on + strand, within Pf1N1B4_905 at 1032.885 kb on - strand, within Pf1N1B4_905 at 1032.885 kb on - strand, within Pf1N1B4_905 at 1032.885 kb on - strand, within Pf1N1B4_905 at 1032.885 kb on - strand, within Pf1N1B4_905 at 1032.911 kb on + strand, within Pf1N1B4_905 at 1032.911 kb on + strand, within Pf1N1B4_905 at 1032.911 kb on + strand, within Pf1N1B4_905 at 1032.911 kb on + strand, within Pf1N1B4_905 at 1032.911 kb on + strand, within Pf1N1B4_905 at 1032.911 kb on + strand, within Pf1N1B4_905 at 1032.911 kb on + strand, within Pf1N1B4_905 at 1032.912 kb on - strand, within Pf1N1B4_905 at 1032.912 kb on - strand, within Pf1N1B4_905 at 1032.912 kb on - strand, within Pf1N1B4_905 at 1032.912 kb on - strand, within Pf1N1B4_905 at 1032.912 kb on - strand, within Pf1N1B4_905 at 1032.912 kb on - strand, within Pf1N1B4_905 at 1032.992 kb on + strand, within Pf1N1B4_905 at 1032.992 kb on + strand, within Pf1N1B4_905 at 1032.992 kb on + strand, within Pf1N1B4_905 at 1032.993 kb on - strand, within Pf1N1B4_905 at 1032.993 kb on - strand, within Pf1N1B4_905 at 1033.280 kb on + strand, within Pf1N1B4_905 at 1033.280 kb on + strand, within Pf1N1B4_905 at 1033.280 kb on + strand, within Pf1N1B4_905 at 1033.281 kb on - strand, within Pf1N1B4_905 at 1033.281 kb on - strand, within Pf1N1B4_905 at 1033.281 kb on - strand, within Pf1N1B4_905 at 1033.281 kb on - strand, within Pf1N1B4_905 at 1033.281 kb on - strand, within Pf1N1B4_905 at 1033.281 kb on - strand, within Pf1N1B4_905 at 1033.281 kb on - strand, within Pf1N1B4_905 at 1033.281 kb on - strand, within Pf1N1B4_905 at 1033.281 kb on - strand, within Pf1N1B4_905 at 1033.281 kb on - strand, within Pf1N1B4_905 at 1033.281 kb on - strand, within Pf1N1B4_905 at 1033.325 kb on + strand, within Pf1N1B4_905 at 1033.469 kb on + strand, within Pf1N1B4_905 at 1033.557 kb on - strand, within Pf1N1B4_905 at 1033.580 kb on + strand, within Pf1N1B4_905 at 1033.581 kb on - strand, within Pf1N1B4_905 at 1033.663 kb on + strand, within Pf1N1B4_905 at 1033.663 kb on + strand, within Pf1N1B4_905 at 1033.664 kb on - strand, within Pf1N1B4_905 at 1033.664 kb on - strand, within Pf1N1B4_905 at 1033.664 kb on - strand, within Pf1N1B4_905 at 1033.664 kb on - strand, within Pf1N1B4_905 at 1033.793 kb on + strand at 1033.794 kb on - strand at 1033.794 kb on - strand at 1033.794 kb on - strand at 1033.794 kb on - strand at 1033.794 kb on - strand at 1033.794 kb on - strand at 1033.794 kb on - strand at 1033.794 kb on - strand at 1033.942 kb on + strand, within Pf1N1B4_906 at 1033.987 kb on + strand, within Pf1N1B4_906 at 1033.987 kb on + strand, within Pf1N1B4_906 at 1033.987 kb on + strand, within Pf1N1B4_906 at 1033.988 kb on - strand, within Pf1N1B4_906 at 1034.032 kb on + strand, within Pf1N1B4_906 at 1034.032 kb on + strand, within Pf1N1B4_906 at 1034.032 kb on + strand, within Pf1N1B4_906 at 1034.033 kb on - strand, within Pf1N1B4_906 at 1034.088 kb on - strand, within Pf1N1B4_906 at 1034.128 kb on + strand at 1034.136 kb on - strand at 1034.168 kb on + strand at 1034.169 kb on - strand at 1034.193 kb on - strand at 1034.563 kb on + strand, within Pf1N1B4_907 at 1034.564 kb on - strand, within Pf1N1B4_907 at 1034.564 kb on - strand, within Pf1N1B4_907 at 1034.713 kb on - strand, within Pf1N1B4_907 at 1034.757 kb on + strand, within Pf1N1B4_907 at 1034.758 kb on - strand, within Pf1N1B4_907 at 1035.002 kb on + strand, within Pf1N1B4_907 at 1035.003 kb on - strand, within Pf1N1B4_907 at 1035.003 kb on - strand, within Pf1N1B4_907 at 1035.003 kb on - strand, within Pf1N1B4_907 at 1035.003 kb on - strand, within Pf1N1B4_907 at 1035.006 kb on + strand, within Pf1N1B4_907 at 1035.006 kb on + strand, within Pf1N1B4_907 at 1035.006 kb on + strand, within Pf1N1B4_907 at 1035.007 kb on - strand, within Pf1N1B4_907 at 1035.007 kb on - strand, within Pf1N1B4_907 at 1035.007 kb on - strand, within Pf1N1B4_907 at 1035.007 kb on - strand, within Pf1N1B4_907 at 1035.007 kb on - strand, within Pf1N1B4_907
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 1,032,884 + +1.4 1,032,884 + Pf1N1B4_905 0.11 +1.4 1,032,884 + Pf1N1B4_905 0.11 -1.7 1,032,884 + Pf1N1B4_905 0.11 -0.7 1,032,884 + -0.2 1,032,884 + Pf1N1B4_905 0.11 -1.3 1,032,885 - Pf1N1B4_905 0.11 -1.0 1,032,885 - Pf1N1B4_905 0.11 +1.1 1,032,885 - Pf1N1B4_905 0.11 -0.3 1,032,885 - Pf1N1B4_905 0.11 -0.8 1,032,911 + Pf1N1B4_905 0.13 +0.7 1,032,911 + Pf1N1B4_905 0.13 +1.0 1,032,911 + Pf1N1B4_905 0.13 +0.4 1,032,911 + Pf1N1B4_905 0.13 +2.1 1,032,911 + Pf1N1B4_905 0.13 -0.9 1,032,911 + Pf1N1B4_905 0.13 +0.1 1,032,911 + Pf1N1B4_905 0.13 +0.1 1,032,912 - Pf1N1B4_905 0.14 -0.2 1,032,912 - Pf1N1B4_905 0.14 -2.1 1,032,912 - Pf1N1B4_905 0.14 +0.8 1,032,912 - Pf1N1B4_905 0.14 -1.6 1,032,912 - Pf1N1B4_905 0.14 -0.9 1,032,912 - Pf1N1B4_905 0.14 -0.2 1,032,992 + Pf1N1B4_905 0.21 +0.3 1,032,992 + Pf1N1B4_905 0.21 -1.8 1,032,992 + Pf1N1B4_905 0.21 -0.3 1,032,993 - Pf1N1B4_905 0.22 -1.4 1,032,993 - Pf1N1B4_905 0.22 +0.1 1,033,280 + Pf1N1B4_905 0.50 +2.4 1,033,280 + Pf1N1B4_905 0.50 -0.0 1,033,280 + Pf1N1B4_905 0.50 +0.1 1,033,281 - Pf1N1B4_905 0.50 -0.8 1,033,281 - Pf1N1B4_905 0.50 +0.5 1,033,281 - Pf1N1B4_905 0.50 -0.4 1,033,281 - Pf1N1B4_905 0.50 -0.3 1,033,281 - Pf1N1B4_905 0.50 -0.2 1,033,281 - Pf1N1B4_905 0.50 +0.5 1,033,281 - Pf1N1B4_905 0.50 +0.0 1,033,281 - Pf1N1B4_905 0.50 +0.6 1,033,281 - Pf1N1B4_905 0.50 +0.4 1,033,281 - Pf1N1B4_905 0.50 -1.6 1,033,281 - Pf1N1B4_905 0.50 +0.8 1,033,325 + Pf1N1B4_905 0.55 -0.2 1,033,469 + Pf1N1B4_905 0.69 +0.6 1,033,557 - Pf1N1B4_905 0.78 -0.4 1,033,580 + Pf1N1B4_905 0.80 -2.2 1,033,581 - Pf1N1B4_905 0.80 -0.3 1,033,663 + Pf1N1B4_905 0.88 -1.4 1,033,663 + Pf1N1B4_905 0.88 +1.4 1,033,664 - Pf1N1B4_905 0.88 -1.2 1,033,664 - Pf1N1B4_905 0.88 -0.5 1,033,664 - Pf1N1B4_905 0.88 +0.9 1,033,664 - Pf1N1B4_905 0.88 -0.9 1,033,793 + -2.3 1,033,794 - -0.1 1,033,794 - -0.1 1,033,794 - -1.2 1,033,794 - -1.4 1,033,794 - -0.5 1,033,794 - -0.2 1,033,794 - -0.1 1,033,794 - -0.5 1,033,942 + Pf1N1B4_906 0.46 -1.9 1,033,987 + Pf1N1B4_906 0.59 -1.6 1,033,987 + Pf1N1B4_906 0.59 -0.7 1,033,987 + Pf1N1B4_906 0.59 +0.4 1,033,988 - Pf1N1B4_906 0.59 -1.4 1,034,032 + Pf1N1B4_906 0.72 -2.1 1,034,032 + Pf1N1B4_906 0.72 +0.3 1,034,032 + Pf1N1B4_906 0.72 -1.8 1,034,033 - Pf1N1B4_906 0.72 -0.2 1,034,088 - Pf1N1B4_906 0.88 -0.1 1,034,128 + +0.2 1,034,136 - +2.4 1,034,168 + +0.9 1,034,169 - +0.8 1,034,193 - +0.0 1,034,563 + Pf1N1B4_907 0.33 -1.3 1,034,564 - Pf1N1B4_907 0.33 +1.4 1,034,564 - Pf1N1B4_907 0.33 -1.2 1,034,713 - Pf1N1B4_907 0.49 -0.1 1,034,757 + Pf1N1B4_907 0.54 -1.1 1,034,758 - Pf1N1B4_907 0.54 -2.6 1,035,002 + Pf1N1B4_907 0.80 -1.3 1,035,003 - Pf1N1B4_907 0.80 +0.4 1,035,003 - Pf1N1B4_907 0.80 +1.1 1,035,003 - Pf1N1B4_907 0.80 +1.1 1,035,003 - Pf1N1B4_907 0.80 +2.4 1,035,006 + Pf1N1B4_907 0.80 -0.8 1,035,006 + Pf1N1B4_907 0.80 +0.9 1,035,006 + Pf1N1B4_907 0.80 -0.7 1,035,007 - Pf1N1B4_907 0.80 -0.6 1,035,007 - Pf1N1B4_907 0.80 -0.9 1,035,007 - Pf1N1B4_907 0.80 +0.0 1,035,007 - Pf1N1B4_907 0.80 +0.2 1,035,007 - Pf1N1B4_907 0.80 -1.2
Or see this region's nucleotide sequence