Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_822 and Pf1N1B4_823 are separated by 201 nucleotides Pf1N1B4_823 and Pf1N1B4_824 are separated by 2 nucleotides Pf1N1B4_824 and Pf1N1B4_825 are separated by 150 nucleotides
Pf1N1B4_822 - ATP-dependent protease La (EC 3.4.21.53) Type I, at 942,860 to 945,277
_822
Pf1N1B4_823 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 945,479 to 945,871
_823
Pf1N1B4_824 - POSSIBLE MEMBRANE PROTEIN, at 945,874 to 946,518
_824
Pf1N1B4_825 - tRNA (uridine-5-oxyacetic acid methyl ester) 34 synthase, at 946,669 to 947,412
_825
Position (kb) 945
946 Strain fitness (log2 ratio) -2
-1
0
1 at 945.439 kb on + strand at 945.439 kb on + strand at 945.439 kb on + strand at 945.439 kb on + strand at 945.440 kb on - strand at 945.440 kb on - strand at 945.440 kb on - strand at 945.477 kb on + strand at 945.477 kb on + strand at 945.477 kb on + strand at 945.478 kb on - strand at 945.500 kb on - strand at 945.704 kb on + strand, within Pf1N1B4_823 at 945.803 kb on + strand, within Pf1N1B4_823 at 945.803 kb on + strand, within Pf1N1B4_823 at 945.803 kb on + strand, within Pf1N1B4_823 at 945.804 kb on - strand, within Pf1N1B4_823 at 946.135 kb on + strand, within Pf1N1B4_824 at 946.135 kb on + strand, within Pf1N1B4_824 at 946.412 kb on + strand, within Pf1N1B4_824 at 946.413 kb on - strand, within Pf1N1B4_824 at 946.413 kb on - strand, within Pf1N1B4_824 at 946.435 kb on + strand, within Pf1N1B4_824 at 946.435 kb on + strand, within Pf1N1B4_824 at 946.435 kb on + strand, within Pf1N1B4_824 at 946.436 kb on - strand, within Pf1N1B4_824 at 946.436 kb on - strand, within Pf1N1B4_824 at 946.436 kb on - strand, within Pf1N1B4_824 at 946.453 kb on + strand, within Pf1N1B4_824 at 946.453 kb on + strand, within Pf1N1B4_824 at 946.454 kb on - strand at 946.852 kb on + strand, within Pf1N1B4_825 at 946.852 kb on + strand, within Pf1N1B4_825
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 945,439 + +0.2 945,439 + -0.0 945,439 + -0.6 945,439 + +1.2 945,440 - -0.1 945,440 - +0.4 945,440 - -1.9 945,477 + +0.3 945,477 + -0.6 945,477 + +1.4 945,478 - -2.2 945,500 - +0.7 945,704 + Pf1N1B4_823 0.57 -0.2 945,803 + Pf1N1B4_823 0.82 -0.8 945,803 + Pf1N1B4_823 0.82 +0.4 945,803 + Pf1N1B4_823 0.82 -1.5 945,804 - Pf1N1B4_823 0.83 -0.4 946,135 + Pf1N1B4_824 0.40 +0.7 946,135 + Pf1N1B4_824 0.40 +0.4 946,412 + Pf1N1B4_824 0.83 +0.4 946,413 - Pf1N1B4_824 0.84 -1.3 946,413 - Pf1N1B4_824 0.84 +0.3 946,435 + Pf1N1B4_824 0.87 -0.3 946,435 + Pf1N1B4_824 0.87 +0.8 946,435 + Pf1N1B4_824 0.87 -1.8 946,436 - Pf1N1B4_824 0.87 +0.0 946,436 - Pf1N1B4_824 0.87 -1.5 946,436 - Pf1N1B4_824 0.87 +0.4 946,453 + Pf1N1B4_824 0.90 -0.1 946,453 + Pf1N1B4_824 0.90 -0.2 946,454 - -0.1 946,852 + Pf1N1B4_825 0.25 +0.1 946,852 + Pf1N1B4_825 0.25 +0.1
Or see this region's nucleotide sequence