Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_769 and Pf1N1B4_770 are separated by 324 nucleotides Pf1N1B4_770 and Pf1N1B4_771 overlap by 18 nucleotides
Pf1N1B4_769 - Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3), at 873,701 to 875,161
_769
Pf1N1B4_770 - hypothetical protein, at 875,486 to 875,626
_770
Pf1N1B4_771 - ABC transporter for L-asparagine and L-glutamate, periplasmic substrate-binding component <small>(from data)</small>, at 875,609 to 876,523
_771
Position (kb) 875
876 Strain fitness (log2 ratio) -2
-1
0
1
2 at 874.589 kb on + strand, within Pf1N1B4_769 at 874.589 kb on + strand, within Pf1N1B4_769 at 874.589 kb on + strand, within Pf1N1B4_769 at 874.589 kb on + strand, within Pf1N1B4_769 at 874.652 kb on + strand, within Pf1N1B4_769 at 874.653 kb on - strand, within Pf1N1B4_769 at 874.766 kb on + strand, within Pf1N1B4_769 at 874.913 kb on + strand, within Pf1N1B4_769 at 874.913 kb on + strand, within Pf1N1B4_769 at 874.913 kb on + strand, within Pf1N1B4_769 at 874.913 kb on + strand, within Pf1N1B4_769 at 874.957 kb on + strand, within Pf1N1B4_769 at 874.991 kb on + strand, within Pf1N1B4_769 at 874.991 kb on + strand, within Pf1N1B4_769 at 875.009 kb on + strand, within Pf1N1B4_769 at 875.009 kb on + strand, within Pf1N1B4_769 at 875.009 kb on + strand, within Pf1N1B4_769 at 875.114 kb on + strand at 875.225 kb on + strand at 875.225 kb on + strand at 875.225 kb on + strand at 875.225 kb on + strand at 875.226 kb on - strand at 875.226 kb on - strand at 875.281 kb on + strand at 875.282 kb on - strand at 875.284 kb on + strand at 875.284 kb on + strand at 875.285 kb on - strand at 875.285 kb on - strand at 875.299 kb on + strand at 875.340 kb on - strand at 875.410 kb on - strand at 875.464 kb on - strand at 875.494 kb on + strand at 875.582 kb on + strand, within Pf1N1B4_770 at 875.582 kb on + strand, within Pf1N1B4_770 at 875.583 kb on - strand, within Pf1N1B4_770 at 875.584 kb on + strand, within Pf1N1B4_770 at 875.584 kb on + strand, within Pf1N1B4_770 at 875.584 kb on + strand, within Pf1N1B4_770 at 875.584 kb on + strand, within Pf1N1B4_770 at 875.762 kb on + strand, within Pf1N1B4_771 at 875.795 kb on + strand, within Pf1N1B4_771 at 875.871 kb on - strand, within Pf1N1B4_771 at 875.871 kb on - strand, within Pf1N1B4_771 at 876.035 kb on + strand, within Pf1N1B4_771 at 876.036 kb on - strand, within Pf1N1B4_771 at 876.036 kb on - strand at 876.036 kb on - strand, within Pf1N1B4_771 at 876.302 kb on + strand, within Pf1N1B4_771 at 876.303 kb on - strand, within Pf1N1B4_771 at 876.371 kb on + strand, within Pf1N1B4_771 at 876.371 kb on + strand, within Pf1N1B4_771 at 876.371 kb on + strand, within Pf1N1B4_771 at 876.371 kb on + strand, within Pf1N1B4_771 at 876.371 kb on + strand, within Pf1N1B4_771 at 876.371 kb on + strand, within Pf1N1B4_771 at 876.371 kb on + strand, within Pf1N1B4_771 at 876.371 kb on + strand, within Pf1N1B4_771 at 876.371 kb on + strand, within Pf1N1B4_771 at 876.372 kb on - strand, within Pf1N1B4_771 at 876.372 kb on - strand, within Pf1N1B4_771 at 876.372 kb on - strand, within Pf1N1B4_771
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 874,589 + Pf1N1B4_769 0.61 -0.1 874,589 + Pf1N1B4_769 0.61 -1.2 874,589 + Pf1N1B4_769 0.61 +0.4 874,589 + Pf1N1B4_769 0.61 +1.0 874,652 + Pf1N1B4_769 0.65 +1.4 874,653 - Pf1N1B4_769 0.65 +1.4 874,766 + Pf1N1B4_769 0.73 -0.9 874,913 + Pf1N1B4_769 0.83 +0.2 874,913 + Pf1N1B4_769 0.83 +1.1 874,913 + Pf1N1B4_769 0.83 +0.8 874,913 + Pf1N1B4_769 0.83 -0.9 874,957 + Pf1N1B4_769 0.86 +2.4 874,991 + Pf1N1B4_769 0.88 -2.1 874,991 + Pf1N1B4_769 0.88 -0.6 875,009 + Pf1N1B4_769 0.90 -1.4 875,009 + Pf1N1B4_769 0.90 -1.4 875,009 + Pf1N1B4_769 0.90 +0.4 875,114 + +0.7 875,225 + -1.6 875,225 + +0.4 875,225 + -0.6 875,225 + -1.6 875,226 - -0.6 875,226 - +0.3 875,281 + +0.2 875,282 - -0.4 875,284 + -0.6 875,284 + -1.1 875,285 - +0.8 875,285 - -0.7 875,299 + +0.3 875,340 - +0.1 875,410 - +0.5 875,464 - -0.1 875,494 + +0.5 875,582 + Pf1N1B4_770 0.68 -0.9 875,582 + Pf1N1B4_770 0.68 -1.3 875,583 - Pf1N1B4_770 0.69 +1.0 875,584 + Pf1N1B4_770 0.70 -1.1 875,584 + Pf1N1B4_770 0.70 -2.1 875,584 + Pf1N1B4_770 0.70 -0.0 875,584 + Pf1N1B4_770 0.70 +0.6 875,762 + Pf1N1B4_771 0.17 -2.3 875,795 + Pf1N1B4_771 0.20 -0.8 875,871 - Pf1N1B4_771 0.29 -1.2 875,871 - Pf1N1B4_771 0.29 -1.4 876,035 + Pf1N1B4_771 0.47 -1.2 876,036 - Pf1N1B4_771 0.47 +0.7 876,036 - -0.6 876,036 - Pf1N1B4_771 0.47 -0.6 876,302 + Pf1N1B4_771 0.76 -1.8 876,303 - Pf1N1B4_771 0.76 -0.1 876,371 + Pf1N1B4_771 0.83 +1.0 876,371 + Pf1N1B4_771 0.83 -0.5 876,371 + Pf1N1B4_771 0.83 +1.1 876,371 + Pf1N1B4_771 0.83 -2.2 876,371 + Pf1N1B4_771 0.83 +0.2 876,371 + Pf1N1B4_771 0.83 -0.3 876,371 + Pf1N1B4_771 0.83 -1.3 876,371 + Pf1N1B4_771 0.83 +0.4 876,371 + Pf1N1B4_771 0.83 -0.4 876,372 - Pf1N1B4_771 0.83 +0.4 876,372 - Pf1N1B4_771 0.83 +0.0 876,372 - Pf1N1B4_771 0.83 -0.8
Or see this region's nucleotide sequence