Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_687 and Pf1N1B4_688 are separated by 0 nucleotides Pf1N1B4_688 and Pf1N1B4_689 are separated by 41 nucleotides Pf1N1B4_689 and Pf1N1B4_690 are separated by 427 nucleotides
Pf1N1B4_687 - Nitrilotriacetate monooxygenase component B (EC 1.14.13.-), at 786,453 to 787,082
_687
Pf1N1B4_688 - FIG00955483: hypothetical protein, at 787,083 to 787,313
_688
Pf1N1B4_689 - FIG00954888: hypothetical protein, at 787,355 to 787,708
_689
Pf1N1B4_690 - Transcriptional regulator, AraC family, at 788,136 to 789,065
_690
Position (kb) 787
788 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 786.396 kb on + strand at 786.397 kb on - strand at 786.452 kb on + strand at 786.452 kb on + strand at 786.453 kb on - strand at 786.453 kb on - strand at 786.474 kb on + strand at 786.474 kb on + strand at 786.474 kb on + strand at 786.475 kb on - strand at 786.475 kb on - strand at 786.593 kb on + strand at 786.593 kb on + strand, within Pf1N1B4_687 at 786.593 kb on + strand, within Pf1N1B4_687 at 786.594 kb on - strand, within Pf1N1B4_687 at 786.594 kb on - strand, within Pf1N1B4_687 at 786.594 kb on - strand, within Pf1N1B4_687 at 786.594 kb on - strand, within Pf1N1B4_687 at 786.594 kb on - strand, within Pf1N1B4_687 at 786.594 kb on - strand, within Pf1N1B4_687 at 786.594 kb on - strand, within Pf1N1B4_687 at 786.594 kb on - strand, within Pf1N1B4_687 at 786.615 kb on + strand, within Pf1N1B4_687 at 786.616 kb on - strand, within Pf1N1B4_687 at 786.616 kb on - strand, within Pf1N1B4_687 at 786.616 kb on - strand, within Pf1N1B4_687 at 786.972 kb on + strand, within Pf1N1B4_687 at 786.972 kb on + strand, within Pf1N1B4_687 at 786.973 kb on - strand, within Pf1N1B4_687 at 787.080 kb on + strand at 787.080 kb on + strand at 787.080 kb on + strand at 787.081 kb on - strand at 787.081 kb on - strand at 787.081 kb on - strand at 787.081 kb on - strand at 787.081 kb on - strand at 787.081 kb on - strand at 787.081 kb on - strand at 787.084 kb on + strand at 787.084 kb on + strand at 787.085 kb on - strand at 787.269 kb on + strand, within Pf1N1B4_688 at 787.269 kb on + strand, within Pf1N1B4_688 at 787.269 kb on + strand, within Pf1N1B4_688 at 787.269 kb on + strand, within Pf1N1B4_688 at 787.269 kb on + strand, within Pf1N1B4_688 at 787.373 kb on + strand at 787.374 kb on - strand at 787.500 kb on + strand, within Pf1N1B4_689 at 787.500 kb on + strand, within Pf1N1B4_689 at 787.500 kb on + strand, within Pf1N1B4_689 at 787.501 kb on - strand, within Pf1N1B4_689 at 787.681 kb on + strand at 787.682 kb on - strand at 787.682 kb on - strand at 787.702 kb on + strand at 787.703 kb on - strand at 787.870 kb on + strand at 787.870 kb on + strand at 788.095 kb on - strand at 788.220 kb on - strand at 788.241 kb on + strand, within Pf1N1B4_690 at 788.241 kb on + strand, within Pf1N1B4_690 at 788.242 kb on - strand at 788.242 kb on - strand, within Pf1N1B4_690 at 788.242 kb on - strand, within Pf1N1B4_690 at 788.242 kb on - strand, within Pf1N1B4_690 at 788.242 kb on - strand, within Pf1N1B4_690 at 788.370 kb on + strand, within Pf1N1B4_690 at 788.371 kb on - strand, within Pf1N1B4_690 at 788.371 kb on - strand, within Pf1N1B4_690 at 788.371 kb on - strand, within Pf1N1B4_690 at 788.371 kb on - strand, within Pf1N1B4_690 at 788.371 kb on - strand, within Pf1N1B4_690 at 788.371 kb on - strand, within Pf1N1B4_690 at 788.379 kb on + strand, within Pf1N1B4_690 at 788.380 kb on - strand, within Pf1N1B4_690 at 788.395 kb on - strand, within Pf1N1B4_690 at 788.395 kb on - strand, within Pf1N1B4_690 at 788.395 kb on - strand, within Pf1N1B4_690 at 788.438 kb on - strand, within Pf1N1B4_690 at 788.438 kb on - strand, within Pf1N1B4_690 at 788.438 kb on - strand, within Pf1N1B4_690 at 788.438 kb on - strand, within Pf1N1B4_690 at 788.562 kb on + strand, within Pf1N1B4_690 at 788.562 kb on + strand, within Pf1N1B4_690 at 788.562 kb on + strand, within Pf1N1B4_690 at 788.562 kb on + strand, within Pf1N1B4_690 at 788.563 kb on - strand, within Pf1N1B4_690
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 786,396 + -0.4 786,397 - +1.4 786,452 + -0.4 786,452 + +0.6 786,453 - -2.1 786,453 - +0.0 786,474 + +0.7 786,474 + -0.9 786,474 + -0.4 786,475 - -1.2 786,475 - -1.2 786,593 + -2.3 786,593 + Pf1N1B4_687 0.22 +1.6 786,593 + Pf1N1B4_687 0.22 -0.6 786,594 - Pf1N1B4_687 0.22 -1.8 786,594 - Pf1N1B4_687 0.22 +2.0 786,594 - Pf1N1B4_687 0.22 +1.0 786,594 - Pf1N1B4_687 0.22 +0.6 786,594 - Pf1N1B4_687 0.22 +0.6 786,594 - Pf1N1B4_687 0.22 +0.0 786,594 - Pf1N1B4_687 0.22 +1.0 786,594 - Pf1N1B4_687 0.22 +1.4 786,615 + Pf1N1B4_687 0.26 -1.3 786,616 - Pf1N1B4_687 0.26 -0.6 786,616 - Pf1N1B4_687 0.26 -0.4 786,616 - Pf1N1B4_687 0.26 -1.0 786,972 + Pf1N1B4_687 0.82 +0.9 786,972 + Pf1N1B4_687 0.82 -1.5 786,973 - Pf1N1B4_687 0.83 -0.3 787,080 + -0.6 787,080 + +0.6 787,080 + -1.2 787,081 - -0.1 787,081 - +0.2 787,081 - -1.4 787,081 - -1.2 787,081 - +0.0 787,081 - +1.0 787,081 - -0.2 787,084 + +1.4 787,084 + +0.3 787,085 - -0.4 787,269 + Pf1N1B4_688 0.81 -0.3 787,269 + Pf1N1B4_688 0.81 +0.6 787,269 + Pf1N1B4_688 0.81 -0.9 787,269 + Pf1N1B4_688 0.81 -0.9 787,269 + Pf1N1B4_688 0.81 +1.4 787,373 + -0.2 787,374 - -4.3 787,500 + Pf1N1B4_689 0.41 -0.3 787,500 + Pf1N1B4_689 0.41 +0.2 787,500 + Pf1N1B4_689 0.41 -0.5 787,501 - Pf1N1B4_689 0.41 -3.3 787,681 + +0.1 787,682 - -0.2 787,682 - +0.4 787,702 + +0.0 787,703 - -1.6 787,870 + -0.8 787,870 + -0.3 788,095 - -0.4 788,220 - -0.0 788,241 + Pf1N1B4_690 0.11 -0.2 788,241 + Pf1N1B4_690 0.11 +0.3 788,242 - +1.4 788,242 - Pf1N1B4_690 0.11 -0.2 788,242 - Pf1N1B4_690 0.11 +0.1 788,242 - Pf1N1B4_690 0.11 +0.6 788,242 - Pf1N1B4_690 0.11 +0.0 788,370 + Pf1N1B4_690 0.25 -0.6 788,371 - Pf1N1B4_690 0.25 -0.5 788,371 - Pf1N1B4_690 0.25 -0.3 788,371 - Pf1N1B4_690 0.25 -0.1 788,371 - Pf1N1B4_690 0.25 -1.3 788,371 - Pf1N1B4_690 0.25 +0.5 788,371 - Pf1N1B4_690 0.25 +0.8 788,379 + Pf1N1B4_690 0.26 -1.3 788,380 - Pf1N1B4_690 0.26 +0.8 788,395 - Pf1N1B4_690 0.28 -0.6 788,395 - Pf1N1B4_690 0.28 +0.1 788,395 - Pf1N1B4_690 0.28 +1.8 788,438 - Pf1N1B4_690 0.32 -1.1 788,438 - Pf1N1B4_690 0.32 +0.4 788,438 - Pf1N1B4_690 0.32 -0.3 788,438 - Pf1N1B4_690 0.32 +0.4 788,562 + Pf1N1B4_690 0.46 +1.4 788,562 + Pf1N1B4_690 0.46 -1.6 788,562 + Pf1N1B4_690 0.46 -0.8 788,562 + Pf1N1B4_690 0.46 -0.2 788,563 - Pf1N1B4_690 0.46 +1.5
Or see this region's nucleotide sequence