Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_598

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_597 and Pf1N1B4_598 are separated by 228 nucleotidesPf1N1B4_598 and Pf1N1B4_599 overlap by 11 nucleotidesPf1N1B4_599 and Pf1N1B4_600 are separated by 177 nucleotides Pf1N1B4_597 - D-mannose isomerase (EC 5.3.1.7) (from data), at 689,093 to 690,352 _597 Pf1N1B4_598 - Integral membrane sensor signal transduction histidine kinase (EC 2.7.13.3), glucose catabolism cluster, at 690,581 to 692,050 _598 Pf1N1B4_599 - DNA-binding response regulator GltR, controls specific porins for the entry of glucose, at 692,040 to 692,771 _599 Pf1N1B4_600 - Glucokinase (EC 2.7.1.2), at 692,949 to 693,908 _600 Position (kb) 690 691 692 693Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 689.637 kb on + strand, within Pf1N1B4_597at 689.638 kb on - strand, within Pf1N1B4_597at 689.643 kb on + strand, within Pf1N1B4_597at 689.643 kb on + strandat 689.644 kb on - strand, within Pf1N1B4_597at 689.644 kb on - strand, within Pf1N1B4_597at 689.644 kb on - strand, within Pf1N1B4_597at 689.691 kb on + strand, within Pf1N1B4_597at 689.692 kb on - strand, within Pf1N1B4_597at 689.692 kb on - strand, within Pf1N1B4_597at 689.692 kb on - strand, within Pf1N1B4_597at 689.692 kb on - strand, within Pf1N1B4_597at 689.829 kb on + strand, within Pf1N1B4_597at 689.857 kb on + strand, within Pf1N1B4_597at 690.000 kb on + strand, within Pf1N1B4_597at 690.000 kb on + strand, within Pf1N1B4_597at 690.163 kb on + strand, within Pf1N1B4_597at 690.164 kb on - strand, within Pf1N1B4_597at 690.164 kb on - strand, within Pf1N1B4_597at 690.373 kb on + strandat 690.373 kb on + strandat 690.374 kb on - strandat 690.374 kb on - strandat 690.374 kb on - strandat 690.390 kb on + strandat 690.391 kb on - strandat 690.432 kb on + strandat 690.432 kb on + strandat 690.432 kb on + strandat 690.468 kb on + strandat 690.468 kb on + strandat 690.468 kb on + strandat 690.468 kb on + strandat 690.468 kb on + strandat 690.468 kb on + strandat 690.468 kb on + strandat 690.468 kb on + strandat 690.468 kb on + strandat 690.468 kb on + strandat 690.469 kb on - strandat 690.469 kb on - strandat 690.469 kb on - strandat 690.469 kb on - strandat 690.593 kb on - strandat 690.847 kb on - strand, within Pf1N1B4_598at 690.967 kb on - strand, within Pf1N1B4_598at 691.455 kb on + strand, within Pf1N1B4_598at 691.482 kb on + strand, within Pf1N1B4_598at 691.482 kb on + strand, within Pf1N1B4_598at 691.482 kb on + strand, within Pf1N1B4_598at 691.482 kb on + strand, within Pf1N1B4_598at 691.483 kb on - strand, within Pf1N1B4_598at 691.483 kb on - strand, within Pf1N1B4_598at 691.483 kb on - strand, within Pf1N1B4_598at 691.483 kb on - strand, within Pf1N1B4_598at 691.483 kb on - strand, within Pf1N1B4_598at 691.557 kb on + strand, within Pf1N1B4_598at 691.557 kb on + strand, within Pf1N1B4_598at 691.791 kb on - strand, within Pf1N1B4_598at 692.081 kb on - strandat 692.458 kb on + strand, within Pf1N1B4_599at 692.701 kb on + strandat 692.701 kb on + strandat 692.701 kb on + strandat 692.701 kb on + strandat 692.701 kb on + strandat 692.702 kb on - strandat 692.702 kb on - strandat 692.702 kb on - strandat 692.702 kb on - strandat 692.702 kb on - strandat 692.722 kb on - strandat 692.722 kb on - strandat 692.807 kb on + strandat 692.807 kb on + strandat 692.807 kb on + strandat 692.807 kb on + strandat 692.807 kb on + strandat 692.808 kb on - strandat 692.808 kb on - strandat 692.814 kb on + strandat 692.814 kb on + strandat 692.828 kb on - strandat 692.828 kb on - strandat 692.844 kb on + strandat 692.844 kb on + strandat 692.845 kb on - strandat 692.845 kb on - strandat 693.040 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
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689,637 + Pf1N1B4_597 0.43 +0.2
689,638 - Pf1N1B4_597 0.43 -0.5
689,643 + Pf1N1B4_597 0.44 -0.4
689,643 + +1.4
689,644 - Pf1N1B4_597 0.44 -0.4
689,644 - Pf1N1B4_597 0.44 +0.5
689,644 - Pf1N1B4_597 0.44 -1.4
689,691 + Pf1N1B4_597 0.47 -0.4
689,692 - Pf1N1B4_597 0.48 -0.8
689,692 - Pf1N1B4_597 0.48 -1.1
689,692 - Pf1N1B4_597 0.48 -0.6
689,692 - Pf1N1B4_597 0.48 +1.0
689,829 + Pf1N1B4_597 0.58 -1.9
689,857 + Pf1N1B4_597 0.61 +1.4
690,000 + Pf1N1B4_597 0.72 -1.4
690,000 + Pf1N1B4_597 0.72 -0.2
690,163 + Pf1N1B4_597 0.85 -1.5
690,164 - Pf1N1B4_597 0.85 +0.5
690,164 - Pf1N1B4_597 0.85 +0.8
690,373 + -0.1
690,373 + -0.0
690,374 - +0.0
690,374 - +1.2
690,374 - -0.2
690,390 + +0.8
690,391 - +0.8
690,432 + -0.1
690,432 + -0.3
690,432 + +0.4
690,468 + +1.0
690,468 + -2.4
690,468 + +0.6
690,468 + +1.4
690,468 + +1.4
690,468 + +2.1
690,468 + +0.1
690,468 + -0.7
690,468 + -0.4
690,468 + +0.2
690,469 - +0.9
690,469 - -2.4
690,469 - +2.4
690,469 - +0.2
690,593 - -0.5
690,847 - Pf1N1B4_598 0.18 -3.4
690,967 - Pf1N1B4_598 0.26 -0.7
691,455 + Pf1N1B4_598 0.59 -1.0
691,482 + Pf1N1B4_598 0.61 +0.4
691,482 + Pf1N1B4_598 0.61 -0.4
691,482 + Pf1N1B4_598 0.61 -0.3
691,482 + Pf1N1B4_598 0.61 -1.4
691,483 - Pf1N1B4_598 0.61 +0.4
691,483 - Pf1N1B4_598 0.61 +0.1
691,483 - Pf1N1B4_598 0.61 -1.9
691,483 - Pf1N1B4_598 0.61 -1.2
691,483 - Pf1N1B4_598 0.61 -0.5
691,557 + Pf1N1B4_598 0.66 -1.1
691,557 + Pf1N1B4_598 0.66 -1.7
691,791 - Pf1N1B4_598 0.82 -0.4
692,081 - +2.4
692,458 + Pf1N1B4_599 0.57 +1.1
692,701 + +0.1
692,701 + -1.2
692,701 + +0.4
692,701 + -0.7
692,701 + +0.8
692,702 - +1.4
692,702 - -0.9
692,702 - -0.6
692,702 - -0.5
692,702 - +0.8
692,722 - -2.1
692,722 - -0.4
692,807 + -2.4
692,807 + -1.3
692,807 + +1.4
692,807 + -0.5
692,807 + -0.9
692,808 - -0.2
692,808 - +1.6
692,814 + +0.6
692,814 + +0.9
692,828 - -1.2
692,828 - -0.0
692,844 + -0.5
692,844 + -0.2
692,845 - +1.4
692,845 - +0.8
693,040 + -0.2

Or see this region's nucleotide sequence