Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5863

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
296,854	+		Pf1N1B4_5862	0.34	+0.2
296,867	+		Pf1N1B4_5862	0.35	+2.4
296,868	-		Pf1N1B4_5862	0.35	-1.4
297,395	-		Pf1N1B4_5862	0.70	-0.0
297,513	+		Pf1N1B4_5862	0.78	+0.3
297,514	-		Pf1N1B4_5862	0.78	+0.1
298,003	-		Pf1N1B4_5863	0.16	+0.2
298,003	-		Pf1N1B4_5863	0.16	-1.5
298,306	-		Pf1N1B4_5863	0.44	-1.6
298,554	+				-0.2
298,583	-		Pf1N1B4_5863	0.71	-2.7
298,583	-		Pf1N1B4_5863	0.71	+0.7
298,887	+				+0.8
298,887	+				+1.4
298,888	-				+0.1
298,890	+				-0.9
298,891	-				-1.4
299,002	+				+0.2
299,003	-				+1.4
299,047	+		Pf1N1B4_5864	0.13	-0.6
299,047	+		Pf1N1B4_5864	0.13	+0.1
299,048	-		Pf1N1B4_5864	0.13	+1.7
299,531	-		Pf1N1B4_5864	0.53	-0.3
299,692	+		Pf1N1B4_5864	0.66	-0.2
299,693	-		Pf1N1B4_5864	0.66	+1.2
299,814	+				-0.9
299,849	+		Pf1N1B4_5864	0.79	-2.1