Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5815 and Pf1N1B4_5816 are separated by 150 nucleotides Pf1N1B4_5816 and Pf1N1B4_5817 are separated by 227 nucleotides Pf1N1B4_5817 and Pf1N1B4_5818 are separated by 192 nucleotides
Pf1N1B4_5815 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 247,219 to 248,796
_5815
Pf1N1B4_5816 - FIG00957304: hypothetical protein, at 248,947 to 249,474
_5816
Pf1N1B4_5817 - Decarboxylase family protein, at 249,702 to 251,075
_5817
Pf1N1B4_5818 - hypothetical protein, at 251,268 to 251,627
_5818
Position (kb) 249
250
251
252 Strain fitness (log2 ratio) -2
-1
0
1 at 248.815 kb on + strand at 248.883 kb on - strand at 248.900 kb on - strand at 248.949 kb on - strand at 249.376 kb on + strand at 249.377 kb on - strand, within Pf1N1B4_5816 at 249.441 kb on - strand at 249.502 kb on - strand at 249.555 kb on + strand at 249.555 kb on + strand at 249.555 kb on + strand at 249.555 kb on + strand at 249.555 kb on + strand at 249.555 kb on + strand at 249.556 kb on - strand at 249.556 kb on - strand at 249.560 kb on - strand at 249.560 kb on - strand at 249.560 kb on - strand at 249.611 kb on - strand at 249.642 kb on + strand at 249.649 kb on + strand at 249.718 kb on + strand at 249.718 kb on + strand at 249.718 kb on + strand at 249.719 kb on - strand at 249.719 kb on - strand at 249.719 kb on - strand at 249.719 kb on - strand at 249.719 kb on - strand at 249.741 kb on - strand at 249.776 kb on - strand at 249.810 kb on + strand at 249.811 kb on - strand at 249.892 kb on - strand, within Pf1N1B4_5817 at 250.203 kb on + strand, within Pf1N1B4_5817 at 250.204 kb on - strand, within Pf1N1B4_5817 at 250.204 kb on - strand at 250.204 kb on - strand, within Pf1N1B4_5817 at 250.507 kb on - strand at 250.650 kb on + strand, within Pf1N1B4_5817 at 250.651 kb on - strand, within Pf1N1B4_5817 at 251.046 kb on + strand at 251.046 kb on + strand at 251.047 kb on - strand at 251.094 kb on + strand at 251.157 kb on - strand at 251.157 kb on - strand at 251.164 kb on - strand at 251.525 kb on - strand, within Pf1N1B4_5818 at 251.525 kb on - strand, within Pf1N1B4_5818 at 251.639 kb on + strand at 251.690 kb on + strand at 251.690 kb on + strand at 251.692 kb on + strand at 251.692 kb on + strand at 251.693 kb on - strand at 251.693 kb on - strand at 251.716 kb on + strand at 251.717 kb on - strand at 251.746 kb on + strand at 251.757 kb on + strand at 251.776 kb on + strand at 251.776 kb on + strand at 251.776 kb on + strand at 251.776 kb on + strand at 251.776 kb on + strand at 251.776 kb on + strand at 251.776 kb on + strand at 251.776 kb on + strand at 251.776 kb on + strand at 251.777 kb on - strand at 251.777 kb on - strand at 251.777 kb on - strand at 251.777 kb on - strand at 251.814 kb on + strand at 251.947 kb on + strand at 251.947 kb on + strand at 251.948 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 248,815 + -1.3 248,883 - -0.2 248,900 - +1.4 248,949 - -1.4 249,376 + +0.8 249,377 - Pf1N1B4_5816 0.81 -0.1 249,441 - -1.3 249,502 - -0.4 249,555 + -0.3 249,555 + +0.6 249,555 + -0.2 249,555 + +0.0 249,555 + -1.6 249,555 + +0.1 249,556 - -0.4 249,556 - -1.2 249,560 - -1.4 249,560 - -0.1 249,560 - -0.3 249,611 - -1.4 249,642 + +0.4 249,649 + -1.2 249,718 + +0.5 249,718 + -0.0 249,718 + -0.4 249,719 - +1.4 249,719 - -0.6 249,719 - +1.6 249,719 - -0.6 249,719 - -1.4 249,741 - -0.2 249,776 - +0.1 249,810 + +0.4 249,811 - -0.3 249,892 - Pf1N1B4_5817 0.14 -0.9 250,203 + Pf1N1B4_5817 0.36 -0.9 250,204 - Pf1N1B4_5817 0.37 +0.2 250,204 - -0.2 250,204 - Pf1N1B4_5817 0.37 -1.4 250,507 - -0.9 250,650 + Pf1N1B4_5817 0.69 +0.6 250,651 - Pf1N1B4_5817 0.69 -1.9 251,046 + +0.6 251,046 + -2.4 251,047 - -0.9 251,094 + +0.4 251,157 - -0.2 251,157 - +0.2 251,164 - +0.1 251,525 - Pf1N1B4_5818 0.71 +0.9 251,525 - Pf1N1B4_5818 0.71 +0.4 251,639 + +0.1 251,690 + +0.6 251,690 + +0.0 251,692 + -0.3 251,692 + -0.5 251,693 - +1.3 251,693 - +1.7 251,716 + +0.6 251,717 - -0.5 251,746 + +1.0 251,757 + -0.7 251,776 + -0.7 251,776 + -1.5 251,776 + -0.4 251,776 + -0.6 251,776 + -0.4 251,776 + -0.1 251,776 + +1.1 251,776 + +1.0 251,776 + -0.9 251,777 - +0.7 251,777 - -0.8 251,777 - -0.0 251,777 - +0.8 251,814 + -0.4 251,947 + -1.4 251,947 + -1.1 251,948 - +0.6
Or see this region's nucleotide sequence