Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_578 and Pf1N1B4_579 are separated by 83 nucleotides Pf1N1B4_579 and Pf1N1B4_580 are separated by 11 nucleotides Pf1N1B4_580 and Pf1N1B4_581 overlap by 14 nucleotides
Pf1N1B4_578 - enoyl-CoA hydratase, R-specific, at 670,949 to 671,419
_578
Pf1N1B4_579 - Membrane fusion component of tripartite multidrug resistance system, at 671,503 to 672,363
_579
Pf1N1B4_580 - FIG00953813: hypothetical protein, at 672,375 to 672,581
_580
Pf1N1B4_581 - membrane protein, putative, at 672,568 to 674,556
_581
Position (kb) 672
673 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 671.422 kb on + strand at 671.422 kb on + strand at 671.423 kb on - strand at 671.458 kb on + strand at 671.458 kb on + strand at 671.665 kb on - strand, within Pf1N1B4_579 at 671.665 kb on - strand, within Pf1N1B4_579 at 671.789 kb on + strand, within Pf1N1B4_579 at 671.801 kb on + strand, within Pf1N1B4_579 at 671.802 kb on - strand, within Pf1N1B4_579 at 671.802 kb on - strand, within Pf1N1B4_579 at 671.802 kb on - strand, within Pf1N1B4_579 at 671.802 kb on - strand, within Pf1N1B4_579 at 671.802 kb on - strand at 671.852 kb on + strand, within Pf1N1B4_579 at 671.852 kb on + strand, within Pf1N1B4_579 at 671.852 kb on + strand, within Pf1N1B4_579 at 671.853 kb on - strand, within Pf1N1B4_579 at 671.853 kb on - strand, within Pf1N1B4_579 at 671.853 kb on - strand, within Pf1N1B4_579 at 671.853 kb on - strand, within Pf1N1B4_579 at 671.853 kb on - strand, within Pf1N1B4_579 at 671.856 kb on + strand, within Pf1N1B4_579 at 671.856 kb on + strand, within Pf1N1B4_579 at 671.857 kb on - strand, within Pf1N1B4_579 at 672.281 kb on + strand at 672.281 kb on + strand at 672.281 kb on + strand at 672.281 kb on + strand at 672.281 kb on + strand at 672.281 kb on + strand at 672.281 kb on + strand at 672.281 kb on + strand at 672.281 kb on + strand at 672.281 kb on + strand at 672.282 kb on - strand at 672.282 kb on - strand at 672.282 kb on - strand at 672.282 kb on - strand at 672.284 kb on + strand at 672.350 kb on - strand at 672.357 kb on + strand at 672.357 kb on + strand at 672.357 kb on + strand at 672.358 kb on - strand at 672.358 kb on - strand at 672.358 kb on - strand at 672.358 kb on - strand at 672.358 kb on - strand at 672.358 kb on - strand at 672.358 kb on - strand at 672.358 kb on - strand at 672.358 kb on - strand at 672.358 kb on - strand at 672.364 kb on - strand at 672.364 kb on - strand at 672.377 kb on - strand at 672.378 kb on + strand at 672.412 kb on - strand, within Pf1N1B4_580 at 672.412 kb on - strand, within Pf1N1B4_580 at 672.412 kb on - strand, within Pf1N1B4_580 at 672.412 kb on - strand, within Pf1N1B4_580 at 672.421 kb on + strand, within Pf1N1B4_580 at 672.421 kb on + strand, within Pf1N1B4_580 at 672.421 kb on + strand at 672.421 kb on + strand, within Pf1N1B4_580 at 672.421 kb on + strand, within Pf1N1B4_580 at 672.421 kb on + strand, within Pf1N1B4_580 at 672.422 kb on - strand, within Pf1N1B4_580 at 672.422 kb on - strand, within Pf1N1B4_580 at 672.422 kb on - strand, within Pf1N1B4_580 at 672.422 kb on - strand, within Pf1N1B4_580 at 672.422 kb on - strand, within Pf1N1B4_580 at 672.422 kb on - strand, within Pf1N1B4_580 at 673.051 kb on - strand, within Pf1N1B4_581 at 673.078 kb on + strand, within Pf1N1B4_581 at 673.174 kb on - strand at 673.174 kb on - strand, within Pf1N1B4_581 at 673.174 kb on - strand, within Pf1N1B4_581
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 671,422 + +0.1 671,422 + -0.8 671,423 - -0.5 671,458 + -1.2 671,458 + -0.1 671,665 - Pf1N1B4_579 0.19 -1.1 671,665 - Pf1N1B4_579 0.19 +1.5 671,789 + Pf1N1B4_579 0.33 -0.6 671,801 + Pf1N1B4_579 0.35 -0.2 671,802 - Pf1N1B4_579 0.35 -0.2 671,802 - Pf1N1B4_579 0.35 +1.0 671,802 - Pf1N1B4_579 0.35 +0.1 671,802 - Pf1N1B4_579 0.35 -0.6 671,802 - -0.2 671,852 + Pf1N1B4_579 0.41 -0.7 671,852 + Pf1N1B4_579 0.41 -0.6 671,852 + Pf1N1B4_579 0.41 -1.4 671,853 - Pf1N1B4_579 0.41 +0.4 671,853 - Pf1N1B4_579 0.41 +0.7 671,853 - Pf1N1B4_579 0.41 +1.7 671,853 - Pf1N1B4_579 0.41 -0.1 671,853 - Pf1N1B4_579 0.41 +1.2 671,856 + Pf1N1B4_579 0.41 +0.8 671,856 + Pf1N1B4_579 0.41 -0.6 671,857 - Pf1N1B4_579 0.41 +0.3 672,281 + +0.2 672,281 + -0.6 672,281 + -1.7 672,281 + +0.9 672,281 + -0.1 672,281 + -0.8 672,281 + +1.7 672,281 + +0.1 672,281 + -0.6 672,281 + +0.3 672,282 - +0.8 672,282 - -1.2 672,282 - -2.1 672,282 - +1.0 672,284 + -0.4 672,350 - -0.5 672,357 + +0.3 672,357 + +0.0 672,357 + -0.6 672,358 - +1.0 672,358 - -0.6 672,358 - +0.8 672,358 - +1.9 672,358 - -0.3 672,358 - +0.4 672,358 - +0.4 672,358 - -0.2 672,358 - -0.6 672,358 - -0.5 672,364 - -1.6 672,364 - -0.4 672,377 - -1.4 672,378 + -0.3 672,412 - Pf1N1B4_580 0.18 -0.3 672,412 - Pf1N1B4_580 0.18 -1.8 672,412 - Pf1N1B4_580 0.18 -0.2 672,412 - Pf1N1B4_580 0.18 -2.0 672,421 + Pf1N1B4_580 0.22 +0.7 672,421 + Pf1N1B4_580 0.22 -0.7 672,421 + -0.9 672,421 + Pf1N1B4_580 0.22 +1.4 672,421 + Pf1N1B4_580 0.22 -1.5 672,421 + Pf1N1B4_580 0.22 -0.9 672,422 - Pf1N1B4_580 0.23 -0.2 672,422 - Pf1N1B4_580 0.23 -0.0 672,422 - Pf1N1B4_580 0.23 -0.3 672,422 - Pf1N1B4_580 0.23 -0.8 672,422 - Pf1N1B4_580 0.23 -2.8 672,422 - Pf1N1B4_580 0.23 -2.1 673,051 - Pf1N1B4_581 0.24 -0.2 673,078 + Pf1N1B4_581 0.26 -0.1 673,174 - +1.4 673,174 - Pf1N1B4_581 0.30 +0.1 673,174 - Pf1N1B4_581 0.30 +0.7
Or see this region's nucleotide sequence