Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5736

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5735 and Pf1N1B4_5736 are separated by 68 nucleotidesPf1N1B4_5736 and Pf1N1B4_5737 overlap by 23 nucleotidesPf1N1B4_5737 and Pf1N1B4_5738 overlap by 4 nucleotides Pf1N1B4_5735 - Respiratory nitrate reductase alpha chain (EC 1.7.99.4), at 174,827 to 178,600 _5735 Pf1N1B4_5736 - Nitrate/nitrite transporter, at 178,669 to 180,066 _5736 Pf1N1B4_5737 - Nitrate/nitrite transporter, at 180,044 to 180,526 _5737 Pf1N1B4_5738 - Nitrate/nitrite transporter, at 180,523 to 181,347 _5738 Position (kb) 178 179 180 181Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 177.750 kb on + strand, within Pf1N1B4_5735at 177.858 kb on + strandat 177.858 kb on + strand, within Pf1N1B4_5735at 177.858 kb on + strand, within Pf1N1B4_5735at 177.858 kb on + strand, within Pf1N1B4_5735at 177.859 kb on - strand, within Pf1N1B4_5735at 177.871 kb on - strand, within Pf1N1B4_5735at 177.951 kb on + strand, within Pf1N1B4_5735at 177.952 kb on - strand, within Pf1N1B4_5735at 177.952 kb on - strand, within Pf1N1B4_5735at 177.988 kb on - strand, within Pf1N1B4_5735at 178.063 kb on - strand, within Pf1N1B4_5735at 178.075 kb on + strand, within Pf1N1B4_5735at 178.076 kb on - strand, within Pf1N1B4_5735at 178.077 kb on + strand, within Pf1N1B4_5735at 178.077 kb on + strand, within Pf1N1B4_5735at 178.078 kb on - strand, within Pf1N1B4_5735at 178.078 kb on - strand, within Pf1N1B4_5735at 178.078 kb on - strand, within Pf1N1B4_5735at 178.156 kb on + strand, within Pf1N1B4_5735at 178.159 kb on - strand, within Pf1N1B4_5735at 178.159 kb on - strand, within Pf1N1B4_5735at 178.290 kb on + strandat 178.291 kb on - strandat 178.296 kb on + strandat 178.296 kb on + strandat 178.414 kb on - strandat 178.589 kb on + strandat 178.590 kb on - strandat 178.733 kb on + strandat 178.751 kb on + strandat 178.752 kb on - strandat 178.752 kb on - strandat 178.752 kb on - strandat 178.752 kb on - strandat 178.752 kb on - strandat 178.752 kb on - strandat 178.752 kb on - strandat 178.752 kb on - strandat 179.406 kb on + strand, within Pf1N1B4_5736at 179.406 kb on + strand, within Pf1N1B4_5736at 179.407 kb on - strand, within Pf1N1B4_5736at 179.407 kb on - strand, within Pf1N1B4_5736at 179.407 kb on - strandat 179.505 kb on + strand, within Pf1N1B4_5736at 179.684 kb on + strand, within Pf1N1B4_5736at 179.798 kb on + strand, within Pf1N1B4_5736at 179.798 kb on + strand, within Pf1N1B4_5736at 179.799 kb on - strand, within Pf1N1B4_5736at 180.144 kb on - strand, within Pf1N1B4_5737at 180.156 kb on + strand, within Pf1N1B4_5737at 180.156 kb on + strand, within Pf1N1B4_5737at 180.156 kb on + strandat 180.157 kb on - strand, within Pf1N1B4_5737at 180.336 kb on + strand, within Pf1N1B4_5737at 180.635 kb on + strand, within Pf1N1B4_5738at 180.636 kb on - strand, within Pf1N1B4_5738at 180.647 kb on + strand, within Pf1N1B4_5738at 180.647 kb on + strand, within Pf1N1B4_5738at 180.647 kb on + strand, within Pf1N1B4_5738at 180.648 kb on - strand, within Pf1N1B4_5738at 180.648 kb on - strand, within Pf1N1B4_5738at 180.648 kb on - strand, within Pf1N1B4_5738at 180.648 kb on - strand, within Pf1N1B4_5738at 180.650 kb on + strand, within Pf1N1B4_5738at 180.650 kb on + strand, within Pf1N1B4_5738at 180.650 kb on + strand, within Pf1N1B4_5738at 180.650 kb on + strand, within Pf1N1B4_5738at 180.651 kb on - strand, within Pf1N1B4_5738at 180.651 kb on - strandat 180.651 kb on - strand, within Pf1N1B4_5738at 180.704 kb on + strand, within Pf1N1B4_5738at 180.705 kb on - strand, within Pf1N1B4_5738at 180.986 kb on + strand, within Pf1N1B4_5738at 180.986 kb on + strand, within Pf1N1B4_5738at 180.987 kb on - strand, within Pf1N1B4_5738at 180.987 kb on - strand, within Pf1N1B4_5738at 180.987 kb on - strand, within Pf1N1B4_5738

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
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177,750 + Pf1N1B4_5735 0.77 -0.4
177,858 + -0.6
177,858 + Pf1N1B4_5735 0.80 -0.6
177,858 + Pf1N1B4_5735 0.80 +0.6
177,858 + Pf1N1B4_5735 0.80 -1.4
177,859 - Pf1N1B4_5735 0.80 -2.2
177,871 - Pf1N1B4_5735 0.81 -1.1
177,951 + Pf1N1B4_5735 0.83 -2.4
177,952 - Pf1N1B4_5735 0.83 -1.7
177,952 - Pf1N1B4_5735 0.83 -0.8
177,988 - Pf1N1B4_5735 0.84 -0.7
178,063 - Pf1N1B4_5735 0.86 -0.8
178,075 + Pf1N1B4_5735 0.86 -1.3
178,076 - Pf1N1B4_5735 0.86 -1.8
178,077 + Pf1N1B4_5735 0.86 -1.1
178,077 + Pf1N1B4_5735 0.86 -0.1
178,078 - Pf1N1B4_5735 0.86 +0.9
178,078 - Pf1N1B4_5735 0.86 -1.6
178,078 - Pf1N1B4_5735 0.86 -0.4
178,156 + Pf1N1B4_5735 0.88 -1.2
178,159 - Pf1N1B4_5735 0.88 -0.9
178,159 - Pf1N1B4_5735 0.88 -1.1
178,290 + -0.4
178,291 - +0.4
178,296 + -0.9
178,296 + -1.9
178,414 - +0.1
178,589 + -0.1
178,590 - -0.5
178,733 + +0.5
178,751 + -1.0
178,752 - -1.1
178,752 - -1.2
178,752 - -0.1
178,752 - -1.2
178,752 - -1.2
178,752 - -0.6
178,752 - +0.7
178,752 - -1.0
179,406 + Pf1N1B4_5736 0.53 -0.7
179,406 + Pf1N1B4_5736 0.53 -1.0
179,407 - Pf1N1B4_5736 0.53 -0.9
179,407 - Pf1N1B4_5736 0.53 -0.2
179,407 - +0.0
179,505 + Pf1N1B4_5736 0.60 -2.1
179,684 + Pf1N1B4_5736 0.73 -1.8
179,798 + Pf1N1B4_5736 0.81 -0.4
179,798 + Pf1N1B4_5736 0.81 +0.2
179,799 - Pf1N1B4_5736 0.81 -0.1
180,144 - Pf1N1B4_5737 0.21 +0.7
180,156 + Pf1N1B4_5737 0.23 -0.4
180,156 + Pf1N1B4_5737 0.23 -0.8
180,156 + +2.2
180,157 - Pf1N1B4_5737 0.23 -0.4
180,336 + Pf1N1B4_5737 0.60 -0.6
180,635 + Pf1N1B4_5738 0.14 -3.0
180,636 - Pf1N1B4_5738 0.14 -0.4
180,647 + Pf1N1B4_5738 0.15 +0.3
180,647 + Pf1N1B4_5738 0.15 +0.0
180,647 + Pf1N1B4_5738 0.15 +0.4
180,648 - Pf1N1B4_5738 0.15 -0.1
180,648 - Pf1N1B4_5738 0.15 -0.4
180,648 - Pf1N1B4_5738 0.15 +0.4
180,648 - Pf1N1B4_5738 0.15 +0.7
180,650 + Pf1N1B4_5738 0.15 -2.2
180,650 + Pf1N1B4_5738 0.15 +1.7
180,650 + Pf1N1B4_5738 0.15 -0.6
180,650 + Pf1N1B4_5738 0.15 -0.8
180,651 - Pf1N1B4_5738 0.16 -0.3
180,651 - +0.4
180,651 - Pf1N1B4_5738 0.16 -1.4
180,704 + Pf1N1B4_5738 0.22 -0.7
180,705 - Pf1N1B4_5738 0.22 -0.1
180,986 + Pf1N1B4_5738 0.56 -0.8
180,986 + Pf1N1B4_5738 0.56 -0.9
180,987 - Pf1N1B4_5738 0.56 +0.5
180,987 - Pf1N1B4_5738 0.56 -0.8
180,987 - Pf1N1B4_5738 0.56 -1.2

Or see this region's nucleotide sequence