Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5732 and Pf1N1B4_5733 overlap by 8 nucleotides Pf1N1B4_5733 and Pf1N1B4_5734 are separated by 2 nucleotides Pf1N1B4_5734 and Pf1N1B4_5735 are separated by 1 nucleotides
Pf1N1B4_5732 - Respiratory nitrate reductase gamma chain (EC 1.7.99.4), at 171,856 to 172,536
_5732
Pf1N1B4_5733 - Respiratory nitrate reductase delta chain (EC 1.7.99.4), at 172,529 to 173,284
_5733
Pf1N1B4_5734 - Respiratory nitrate reductase beta chain (EC 1.7.99.4), at 173,287 to 174,825
_5734
Pf1N1B4_5735 - Respiratory nitrate reductase alpha chain (EC 1.7.99.4), at 174,827 to 178,600
_5735
Position (kb) 173
174
175 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 172.502 kb on + strand at 173.474 kb on + strand, within Pf1N1B4_5734 at 173.475 kb on - strand, within Pf1N1B4_5734 at 173.475 kb on - strand, within Pf1N1B4_5734 at 173.499 kb on - strand, within Pf1N1B4_5734 at 173.499 kb on - strand at 173.499 kb on - strand, within Pf1N1B4_5734 at 173.663 kb on + strand, within Pf1N1B4_5734 at 173.663 kb on + strand, within Pf1N1B4_5734 at 173.664 kb on - strand, within Pf1N1B4_5734 at 173.664 kb on - strand, within Pf1N1B4_5734 at 173.838 kb on - strand, within Pf1N1B4_5734 at 174.132 kb on - strand, within Pf1N1B4_5734 at 174.224 kb on + strand, within Pf1N1B4_5734 at 174.293 kb on + strand, within Pf1N1B4_5734 at 174.293 kb on + strand at 174.293 kb on + strand, within Pf1N1B4_5734 at 174.294 kb on - strand, within Pf1N1B4_5734 at 174.294 kb on - strand, within Pf1N1B4_5734 at 174.294 kb on - strand, within Pf1N1B4_5734 at 174.525 kb on - strand, within Pf1N1B4_5734 at 174.528 kb on - strand, within Pf1N1B4_5734 at 174.714 kb on - strand at 174.955 kb on - strand at 174.957 kb on + strand at 174.958 kb on - strand at 174.958 kb on - strand at 174.958 kb on - strand at 175.101 kb on + strand at 175.102 kb on - strand at 175.284 kb on + strand, within Pf1N1B4_5735 at 175.285 kb on - strand, within Pf1N1B4_5735 at 175.522 kb on - strand, within Pf1N1B4_5735
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 172,502 + -1.4 173,474 + Pf1N1B4_5734 0.12 -0.6 173,475 - Pf1N1B4_5734 0.12 -2.1 173,475 - Pf1N1B4_5734 0.12 -1.1 173,499 - Pf1N1B4_5734 0.14 -2.9 173,499 - -1.8 173,499 - Pf1N1B4_5734 0.14 -1.4 173,663 + Pf1N1B4_5734 0.24 -1.1 173,663 + Pf1N1B4_5734 0.24 +0.8 173,664 - Pf1N1B4_5734 0.24 -0.4 173,664 - Pf1N1B4_5734 0.24 -1.0 173,838 - Pf1N1B4_5734 0.36 -1.8 174,132 - Pf1N1B4_5734 0.55 -0.2 174,224 + Pf1N1B4_5734 0.61 -0.8 174,293 + Pf1N1B4_5734 0.65 -0.2 174,293 + +0.8 174,293 + Pf1N1B4_5734 0.65 -0.4 174,294 - Pf1N1B4_5734 0.65 +2.2 174,294 - Pf1N1B4_5734 0.65 -0.3 174,294 - Pf1N1B4_5734 0.65 -0.4 174,525 - Pf1N1B4_5734 0.80 -1.1 174,528 - Pf1N1B4_5734 0.81 -0.2 174,714 - -0.5 174,955 - +0.2 174,957 + -0.2 174,958 - -1.4 174,958 - +0.6 174,958 - -2.4 175,101 + -1.0 175,102 - -0.2 175,284 + Pf1N1B4_5735 0.12 -0.9 175,285 - Pf1N1B4_5735 0.12 -0.5 175,522 - Pf1N1B4_5735 0.18 -2.3
Or see this region's nucleotide sequence