Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5726 and Pf1N1B4_5727 are separated by 15 nucleotides Pf1N1B4_5727 and Pf1N1B4_5728 are separated by 5 nucleotides Pf1N1B4_5728 and Pf1N1B4_5729 are separated by 32 nucleotides Pf1N1B4_5729 and Pf1N1B4_5730 are separated by 277 nucleotides
Pf1N1B4_5726 - Ferredoxin, at 165,339 to 166,667
_5726
Pf1N1B4_5727 - Ribonucleotide reductase of class III (anaerobic), activating protein (EC 1.97.1.4), at 166,683 to 167,369
_5727
Pf1N1B4_5728 - hypothetical protein, at 167,375 to 167,542
_5728
Pf1N1B4_5729 - Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2), at 167,575 to 169,590
_5729
Pf1N1B4_5730 - Molybdenum cofactor biosynthesis protein MoaA, at 169,868 to 170,860
_5730
Position (kb) 167
168
169
170 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 166.906 kb on + strand, within Pf1N1B4_5727 at 167.781 kb on - strand, within Pf1N1B4_5729 at 167.781 kb on - strand, within Pf1N1B4_5729 at 167.781 kb on - strand at 167.781 kb on - strand, within Pf1N1B4_5729 at 167.891 kb on + strand, within Pf1N1B4_5729 at 167.891 kb on + strand, within Pf1N1B4_5729 at 167.891 kb on + strand, within Pf1N1B4_5729 at 167.894 kb on + strand, within Pf1N1B4_5729 at 167.999 kb on + strand at 168.094 kb on - strand, within Pf1N1B4_5729 at 168.194 kb on + strand, within Pf1N1B4_5729 at 168.194 kb on + strand, within Pf1N1B4_5729 at 168.320 kb on + strand, within Pf1N1B4_5729 at 168.320 kb on + strand, within Pf1N1B4_5729 at 168.572 kb on + strand, within Pf1N1B4_5729 at 168.573 kb on - strand, within Pf1N1B4_5729 at 168.635 kb on + strand, within Pf1N1B4_5729 at 168.635 kb on + strand, within Pf1N1B4_5729 at 168.806 kb on + strand, within Pf1N1B4_5729 at 168.806 kb on + strand, within Pf1N1B4_5729 at 168.806 kb on + strand at 168.866 kb on + strand, within Pf1N1B4_5729 at 168.866 kb on + strand, within Pf1N1B4_5729 at 169.127 kb on + strand, within Pf1N1B4_5729 at 169.127 kb on + strand, within Pf1N1B4_5729 at 169.127 kb on + strand, within Pf1N1B4_5729 at 169.128 kb on - strand at 169.146 kb on - strand, within Pf1N1B4_5729 at 169.244 kb on + strand, within Pf1N1B4_5729 at 169.325 kb on + strand, within Pf1N1B4_5729 at 169.466 kb on + strand at 169.467 kb on - strand at 169.561 kb on - strand at 169.606 kb on + strand at 169.607 kb on - strand at 169.607 kb on - strand at 169.607 kb on - strand at 169.607 kb on - strand at 169.607 kb on - strand at 169.607 kb on - strand at 169.610 kb on + strand at 169.610 kb on + strand at 169.611 kb on - strand at 169.611 kb on - strand at 169.611 kb on - strand at 169.611 kb on - strand at 169.611 kb on - strand at 169.612 kb on + strand at 169.613 kb on - strand at 169.613 kb on - strand at 169.613 kb on - strand at 169.613 kb on - strand at 169.613 kb on - strand at 169.759 kb on - strand at 169.783 kb on + strand at 169.784 kb on - strand at 169.784 kb on - strand at 169.784 kb on - strand at 169.844 kb on - strand at 170.083 kb on + strand, within Pf1N1B4_5730 at 170.160 kb on + strand, within Pf1N1B4_5730 at 170.161 kb on - strand, within Pf1N1B4_5730 at 170.161 kb on - strand, within Pf1N1B4_5730 at 170.329 kb on - strand, within Pf1N1B4_5730
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 166,906 + Pf1N1B4_5727 0.32 +0.7 167,781 - Pf1N1B4_5729 0.10 -2.8 167,781 - Pf1N1B4_5729 0.10 +0.4 167,781 - -1.2 167,781 - Pf1N1B4_5729 0.10 -0.4 167,891 + Pf1N1B4_5729 0.16 -0.2 167,891 + Pf1N1B4_5729 0.16 -1.8 167,891 + Pf1N1B4_5729 0.16 -0.5 167,894 + Pf1N1B4_5729 0.16 -1.1 167,999 + -0.5 168,094 - Pf1N1B4_5729 0.26 -2.2 168,194 + Pf1N1B4_5729 0.31 -1.1 168,194 + Pf1N1B4_5729 0.31 +0.3 168,320 + Pf1N1B4_5729 0.37 -0.6 168,320 + Pf1N1B4_5729 0.37 -1.4 168,572 + Pf1N1B4_5729 0.49 -1.5 168,573 - Pf1N1B4_5729 0.50 -0.8 168,635 + Pf1N1B4_5729 0.53 -0.8 168,635 + Pf1N1B4_5729 0.53 +0.0 168,806 + Pf1N1B4_5729 0.61 -0.9 168,806 + Pf1N1B4_5729 0.61 -1.8 168,806 + -0.2 168,866 + Pf1N1B4_5729 0.64 -1.0 168,866 + Pf1N1B4_5729 0.64 -1.3 169,127 + Pf1N1B4_5729 0.77 +0.5 169,127 + Pf1N1B4_5729 0.77 -1.4 169,127 + Pf1N1B4_5729 0.77 -0.5 169,128 - -0.0 169,146 - Pf1N1B4_5729 0.78 +0.1 169,244 + Pf1N1B4_5729 0.83 -1.0 169,325 + Pf1N1B4_5729 0.87 -0.1 169,466 + -1.9 169,467 - -1.1 169,561 - +0.8 169,606 + +0.1 169,607 - -0.2 169,607 - -1.0 169,607 - -2.6 169,607 - -1.4 169,607 - +1.4 169,607 - +1.9 169,610 + -0.4 169,610 + +0.8 169,611 - -0.6 169,611 - -1.6 169,611 - -3.4 169,611 - +0.2 169,611 - +0.4 169,612 + -0.4 169,613 - -0.2 169,613 - +0.1 169,613 - +1.1 169,613 - -2.9 169,613 - +1.0 169,759 - +0.1 169,783 + -1.8 169,784 - -0.6 169,784 - +0.1 169,784 - +0.5 169,844 - -1.3 170,083 + Pf1N1B4_5730 0.22 +1.8 170,160 + Pf1N1B4_5730 0.29 -0.3 170,161 - Pf1N1B4_5730 0.30 -1.4 170,161 - Pf1N1B4_5730 0.30 -1.6 170,329 - Pf1N1B4_5730 0.46 +0.8
Or see this region's nucleotide sequence