Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5707 and Pf1N1B4_5708 are separated by 84 nucleotides Pf1N1B4_5708 and Pf1N1B4_5709 are separated by 355 nucleotides
Pf1N1B4_5707 - NADH dehydrogenase (EC 1.6.99.3), at 145,671 to 146,762
_5707
Pf1N1B4_5708 - Transcriptional regulator, TetR family, at 146,847 to 147,461
_5708
Pf1N1B4_5709 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 147,817 to 149,052
_5709
Position (kb) 146
147
148 Strain fitness (log2 ratio) -2
-1
0
1 at 145.847 kb on + strand, within Pf1N1B4_5707 at 146.047 kb on + strand, within Pf1N1B4_5707 at 146.048 kb on - strand, within Pf1N1B4_5707 at 146.048 kb on - strand, within Pf1N1B4_5707 at 146.048 kb on - strand at 146.082 kb on - strand, within Pf1N1B4_5707 at 146.132 kb on + strand, within Pf1N1B4_5707 at 146.132 kb on + strand, within Pf1N1B4_5707 at 146.132 kb on + strand, within Pf1N1B4_5707 at 146.133 kb on - strand, within Pf1N1B4_5707 at 146.133 kb on - strand, within Pf1N1B4_5707 at 146.151 kb on + strand, within Pf1N1B4_5707 at 146.152 kb on - strand, within Pf1N1B4_5707 at 146.190 kb on - strand, within Pf1N1B4_5707 at 146.348 kb on + strand, within Pf1N1B4_5707 at 146.348 kb on + strand, within Pf1N1B4_5707 at 146.349 kb on - strand at 146.549 kb on - strand at 146.555 kb on - strand, within Pf1N1B4_5707 at 146.555 kb on - strand, within Pf1N1B4_5707 at 146.582 kb on - strand, within Pf1N1B4_5707 at 146.790 kb on - strand at 147.330 kb on - strand, within Pf1N1B4_5708 at 147.332 kb on - strand, within Pf1N1B4_5708 at 147.332 kb on - strand, within Pf1N1B4_5708 at 147.480 kb on - strand at 147.567 kb on + strand at 147.567 kb on + strand at 147.567 kb on + strand at 147.590 kb on + strand at 147.591 kb on - strand at 147.591 kb on - strand at 147.591 kb on - strand at 147.623 kb on + strand at 147.747 kb on - strand at 148.148 kb on + strand, within Pf1N1B4_5709 at 148.148 kb on + strand, within Pf1N1B4_5709 at 148.149 kb on - strand, within Pf1N1B4_5709 at 148.278 kb on + strand, within Pf1N1B4_5709
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 145,847 + Pf1N1B4_5707 0.16 +0.2 146,047 + Pf1N1B4_5707 0.34 -0.5 146,048 - Pf1N1B4_5707 0.35 -0.8 146,048 - Pf1N1B4_5707 0.35 -0.2 146,048 - -2.0 146,082 - Pf1N1B4_5707 0.38 +1.4 146,132 + Pf1N1B4_5707 0.42 -0.6 146,132 + Pf1N1B4_5707 0.42 -1.2 146,132 + Pf1N1B4_5707 0.42 -0.2 146,133 - Pf1N1B4_5707 0.42 -0.4 146,133 - Pf1N1B4_5707 0.42 +0.4 146,151 + Pf1N1B4_5707 0.44 -0.3 146,152 - Pf1N1B4_5707 0.44 -1.2 146,190 - Pf1N1B4_5707 0.48 +0.8 146,348 + Pf1N1B4_5707 0.62 -1.2 146,348 + Pf1N1B4_5707 0.62 -1.0 146,349 - +0.6 146,549 - +0.1 146,555 - Pf1N1B4_5707 0.81 +1.0 146,555 - Pf1N1B4_5707 0.81 -2.0 146,582 - Pf1N1B4_5707 0.83 +0.4 146,790 - -0.9 147,330 - Pf1N1B4_5708 0.79 -2.6 147,332 - Pf1N1B4_5708 0.79 -0.2 147,332 - Pf1N1B4_5708 0.79 -2.2 147,480 - -0.1 147,567 + +1.2 147,567 + -0.2 147,567 + -0.4 147,590 + +0.3 147,591 - -0.8 147,591 - -0.1 147,591 - -1.0 147,623 + -1.8 147,747 - +0.4 148,148 + Pf1N1B4_5709 0.27 +1.4 148,148 + Pf1N1B4_5709 0.27 +0.0 148,149 - Pf1N1B4_5709 0.27 -0.6 148,278 + Pf1N1B4_5709 0.37 +1.7
Or see this region's nucleotide sequence