Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5401 and Pf1N1B4_5402 overlap by 1 nucleotides
Pf1N1B4_5401 - hypothetical protein, at 134,226 to 135,608
_5401
Pf1N1B4_5402 - hypothetical protein, at 135,608 to 135,892
_5402
Position (kb)
135
136 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 134.698 kb on + strand, within Pf1N1B4_5401 at 134.699 kb on - strand, within Pf1N1B4_5401 at 134.699 kb on - strand, within Pf1N1B4_5401 at 134.863 kb on + strand, within Pf1N1B4_5401 at 134.864 kb on - strand, within Pf1N1B4_5401 at 134.900 kb on - strand, within Pf1N1B4_5401 at 135.039 kb on + strand, within Pf1N1B4_5401 at 135.040 kb on - strand, within Pf1N1B4_5401 at 135.043 kb on + strand, within Pf1N1B4_5401 at 135.043 kb on + strand, within Pf1N1B4_5401 at 135.074 kb on + strand, within Pf1N1B4_5401 at 135.099 kb on + strand, within Pf1N1B4_5401 at 135.100 kb on - strand, within Pf1N1B4_5401 at 135.100 kb on - strand, within Pf1N1B4_5401 at 135.119 kb on - strand, within Pf1N1B4_5401 at 135.430 kb on + strand, within Pf1N1B4_5401 at 135.430 kb on + strand, within Pf1N1B4_5401 at 135.430 kb on + strand, within Pf1N1B4_5401 at 135.431 kb on - strand, within Pf1N1B4_5401 at 135.431 kb on - strand, within Pf1N1B4_5401 at 135.448 kb on + strand, within Pf1N1B4_5401 at 135.449 kb on - strand, within Pf1N1B4_5401 at 135.482 kb on + strand at 135.482 kb on + strand at 135.482 kb on + strand at 135.482 kb on + strand at 135.482 kb on + strand at 135.482 kb on + strand at 135.482 kb on + strand at 135.483 kb on - strand at 135.483 kb on - strand at 135.483 kb on - strand at 135.483 kb on - strand at 135.483 kb on - strand at 135.491 kb on - strand at 135.491 kb on - strand at 135.491 kb on - strand at 135.625 kb on + strand at 135.625 kb on + strand at 135.625 kb on + strand at 135.626 kb on - strand at 135.631 kb on - strand at 135.692 kb on + strand, within Pf1N1B4_5402 at 135.692 kb on + strand, within Pf1N1B4_5402 at 135.693 kb on - strand at 135.693 kb on - strand, within Pf1N1B4_5402 at 135.693 kb on - strand, within Pf1N1B4_5402 at 135.748 kb on + strand, within Pf1N1B4_5402 at 135.808 kb on + strand at 135.809 kb on - strand, within Pf1N1B4_5402 at 135.809 kb on - strand, within Pf1N1B4_5402 at 135.919 kb on - strand at 135.940 kb on - strand at 135.994 kb on - strand at 135.994 kb on - strand at 135.994 kb on - strand at 136.011 kb on - strand at 136.011 kb on - strand at 136.050 kb on + strand at 136.050 kb on + strand at 136.051 kb on - strand at 136.051 kb on - strand at 136.138 kb on + strand at 136.139 kb on - strand at 136.261 kb on - strand at 136.261 kb on - strand at 136.307 kb on + strand at 136.307 kb on + strand at 136.308 kb on - strand at 136.308 kb on - strand at 136.308 kb on - strand at 136.308 kb on - strand at 136.309 kb on + strand at 136.353 kb on + strand at 136.448 kb on + strand at 136.449 kb on - strand at 136.449 kb on - strand at 136.449 kb on - strand at 136.449 kb on - strand at 136.555 kb on + strand at 136.555 kb on + strand at 136.555 kb on + strand at 136.555 kb on + strand at 136.555 kb on + strand at 136.555 kb on + strand at 136.555 kb on + strand at 136.556 kb on - strand at 136.556 kb on - strand at 136.556 kb on - strand at 136.559 kb on + strand at 136.559 kb on + strand at 136.559 kb on + strand at 136.559 kb on + strand at 136.559 kb on + strand at 136.559 kb on + strand at 136.559 kb on + strand at 136.559 kb on + strand at 136.559 kb on + strand at 136.559 kb on + strand at 136.560 kb on - strand at 136.560 kb on - strand at 136.560 kb on - strand at 136.560 kb on - strand at 136.560 kb on - strand at 136.560 kb on - strand at 136.560 kb on - strand at 136.560 kb on - strand at 136.768 kb on + strand at 136.768 kb on + strand at 136.768 kb on + strand at 136.768 kb on + strand at 136.768 kb on + strand at 136.769 kb on - strand at 136.769 kb on - strand at 136.861 kb on + strand at 136.861 kb on + strand at 136.862 kb on - strand at 136.888 kb on + strand at 136.888 kb on + strand at 136.888 kb on + strand at 136.888 kb on + strand at 136.888 kb on + strand at 136.888 kb on + strand at 136.888 kb on + strand at 136.888 kb on + strand at 136.888 kb on + strand at 136.888 kb on + strand at 136.889 kb on - strand at 136.889 kb on - strand at 136.889 kb on - strand at 136.892 kb on + strand at 136.892 kb on + strand at 136.892 kb on + strand at 136.892 kb on + strand at 136.892 kb on + strand at 136.892 kb on + strand at 136.892 kb on + strand at 136.892 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 134,698 + Pf1N1B4_5401 0.34 +1.4 134,699 - Pf1N1B4_5401 0.34 -0.6 134,699 - Pf1N1B4_5401 0.34 -2.3 134,863 + Pf1N1B4_5401 0.46 +3.4 134,864 - Pf1N1B4_5401 0.46 +0.4 134,900 - Pf1N1B4_5401 0.49 -0.9 135,039 + Pf1N1B4_5401 0.59 -0.7 135,040 - Pf1N1B4_5401 0.59 +0.1 135,043 + Pf1N1B4_5401 0.59 +0.2 135,043 + Pf1N1B4_5401 0.59 -1.2 135,074 + Pf1N1B4_5401 0.61 +0.2 135,099 + Pf1N1B4_5401 0.63 +0.0 135,100 - Pf1N1B4_5401 0.63 -4.2 135,100 - Pf1N1B4_5401 0.63 +0.4 135,119 - Pf1N1B4_5401 0.65 -0.3 135,430 + Pf1N1B4_5401 0.87 -0.2 135,430 + Pf1N1B4_5401 0.87 -0.1 135,430 + Pf1N1B4_5401 0.87 -0.2 135,431 - Pf1N1B4_5401 0.87 +0.2 135,431 - Pf1N1B4_5401 0.87 -0.4 135,448 + Pf1N1B4_5401 0.88 -2.8 135,449 - Pf1N1B4_5401 0.88 +0.6 135,482 + -0.9 135,482 + -1.2 135,482 + +0.5 135,482 + +0.1 135,482 + +2.0 135,482 + +0.1 135,482 + -1.8 135,483 - -0.2 135,483 - -0.1 135,483 - +0.1 135,483 - -1.4 135,483 - -1.2 135,491 - -0.0 135,491 - -1.4 135,491 - +0.0 135,625 + -0.0 135,625 + -0.4 135,625 + -0.2 135,626 - +0.6 135,631 - -1.6 135,692 + Pf1N1B4_5402 0.29 -2.2 135,692 + Pf1N1B4_5402 0.29 -0.4 135,693 - +0.2 135,693 - Pf1N1B4_5402 0.30 -1.3 135,693 - Pf1N1B4_5402 0.30 -0.9 135,748 + Pf1N1B4_5402 0.49 -0.6 135,808 + -0.8 135,809 - Pf1N1B4_5402 0.71 -0.2 135,809 - Pf1N1B4_5402 0.71 +1.0 135,919 - -2.1 135,940 - -1.8 135,994 - -1.6 135,994 - +0.8 135,994 - +0.1 136,011 - +0.4 136,011 - -1.9 136,050 + -0.2 136,050 + +1.0 136,051 - -2.2 136,051 - +0.9 136,138 + -2.3 136,139 - +0.8 136,261 - -0.2 136,261 - +1.1 136,307 + -1.2 136,307 + +0.6 136,308 - -0.2 136,308 - -0.3 136,308 - +0.6 136,308 - -2.2 136,309 + -1.5 136,353 + -0.2 136,448 + -0.4 136,449 - +0.1 136,449 - +0.0 136,449 - -1.9 136,449 - -0.6 136,555 + -1.1 136,555 + -0.9 136,555 + -1.8 136,555 + +0.4 136,555 + -0.4 136,555 + -2.3 136,555 + -1.1 136,556 - +0.1 136,556 - -1.7 136,556 - +0.0 136,559 + +0.1 136,559 + -0.9 136,559 + +0.8 136,559 + +1.1 136,559 + +0.7 136,559 + +0.7 136,559 + +0.8 136,559 + -1.1 136,559 + -1.1 136,559 + +1.4 136,560 - +2.0 136,560 - -0.8 136,560 - -1.9 136,560 - -1.8 136,560 - -0.8 136,560 - -1.8 136,560 - +1.4 136,560 - +0.1 136,768 + -0.6 136,768 + -0.0 136,768 + -0.2 136,768 + -0.2 136,768 + +1.4 136,769 - +3.0 136,769 - -1.6 136,861 + -0.9 136,861 + +0.9 136,862 - -0.7 136,888 + +0.4 136,888 + -1.6 136,888 + +0.3 136,888 + +1.3 136,888 + -1.2 136,888 + +0.7 136,888 + -2.1 136,888 + +1.0 136,888 + -0.4 136,888 + -0.5 136,889 - -1.1 136,889 - -0.6 136,889 - -0.3 136,892 + -0.4 136,892 + +0.7 136,892 + -0.2 136,892 + -0.6 136,892 + -0.4 136,892 + +0.4 136,892 + -0.4 136,892 + +0.1
Or see this region's nucleotide sequence