Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_537 and Pf1N1B4_538 are separated by 76 nucleotides Pf1N1B4_538 and Pf1N1B4_539 are separated by 120 nucleotides Pf1N1B4_539 and Pf1N1B4_540 are separated by 201 nucleotides
Pf1N1B4_537 - FIG00959721: hypothetical protein, at 635,559 to 636,275
_537
Pf1N1B4_538 - hypothetical protein, at 636,352 to 637,221
_538
Pf1N1B4_539 - Transcriptional regulators, at 637,342 to 637,734
_539
Pf1N1B4_540 - FIG00960825: hypothetical protein, at 637,936 to 638,670
_540
Position (kb) 636
637
638 Strain fitness (log2 ratio) -2
-1
0
1
2 at 635.361 kb on + strand at 635.500 kb on + strand at 635.500 kb on + strand at 635.518 kb on - strand at 635.760 kb on + strand at 635.760 kb on + strand, within Pf1N1B4_537 at 635.760 kb on + strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.823 kb on + strand, within Pf1N1B4_537 at 635.823 kb on + strand, within Pf1N1B4_537 at 635.824 kb on - strand, within Pf1N1B4_537 at 635.824 kb on - strand, within Pf1N1B4_537 at 635.824 kb on - strand, within Pf1N1B4_537 at 635.834 kb on + strand, within Pf1N1B4_537 at 636.054 kb on + strand, within Pf1N1B4_537 at 636.054 kb on + strand, within Pf1N1B4_537 at 636.054 kb on + strand, within Pf1N1B4_537 at 636.055 kb on - strand, within Pf1N1B4_537 at 636.099 kb on + strand, within Pf1N1B4_537 at 636.099 kb on + strand, within Pf1N1B4_537 at 636.100 kb on - strand, within Pf1N1B4_537 at 636.100 kb on - strand, within Pf1N1B4_537 at 636.114 kb on - strand, within Pf1N1B4_537 at 636.171 kb on + strand, within Pf1N1B4_537 at 636.171 kb on + strand, within Pf1N1B4_537 at 636.172 kb on - strand, within Pf1N1B4_537 at 636.210 kb on + strand at 636.224 kb on + strand at 636.224 kb on + strand at 636.270 kb on + strand at 636.270 kb on + strand at 636.271 kb on - strand at 636.271 kb on - strand at 636.271 kb on - strand at 636.271 kb on - strand at 636.271 kb on - strand at 636.273 kb on + strand at 636.273 kb on + strand at 636.475 kb on + strand, within Pf1N1B4_538 at 636.476 kb on - strand, within Pf1N1B4_538 at 636.699 kb on - strand at 636.784 kb on + strand, within Pf1N1B4_538 at 637.219 kb on + strand at 637.219 kb on + strand at 637.219 kb on + strand at 637.219 kb on + strand at 637.219 kb on + strand at 637.220 kb on - strand at 637.226 kb on + strand at 637.232 kb on + strand at 637.233 kb on - strand at 637.233 kb on - strand at 637.494 kb on - strand, within Pf1N1B4_539 at 637.539 kb on - strand, within Pf1N1B4_539 at 637.638 kb on - strand at 637.649 kb on + strand, within Pf1N1B4_539 at 637.649 kb on + strand, within Pf1N1B4_539 at 637.650 kb on - strand, within Pf1N1B4_539 at 637.723 kb on - strand at 637.723 kb on - strand at 637.723 kb on - strand at 637.724 kb on + strand at 637.724 kb on + strand at 637.724 kb on + strand at 637.724 kb on + strand at 637.724 kb on + strand at 637.725 kb on - strand at 637.725 kb on - strand at 637.725 kb on - strand at 637.725 kb on - strand at 637.725 kb on - strand at 637.725 kb on - strand at 637.725 kb on - strand at 637.725 kb on - strand at 637.725 kb on - strand at 637.775 kb on + strand at 637.775 kb on + strand at 637.775 kb on + strand at 637.775 kb on + strand at 637.776 kb on - strand at 637.776 kb on - strand at 637.776 kb on - strand at 637.776 kb on - strand at 637.776 kb on - strand at 637.776 kb on - strand at 637.776 kb on - strand at 637.776 kb on - strand at 637.776 kb on - strand at 637.776 kb on - strand at 637.776 kb on - strand at 637.815 kb on + strand at 637.868 kb on - strand at 637.876 kb on + strand at 637.876 kb on + strand at 637.876 kb on + strand at 637.877 kb on - strand at 637.877 kb on - strand at 637.877 kb on - strand at 637.877 kb on - strand at 637.886 kb on + strand at 638.158 kb on + strand, within Pf1N1B4_540 at 638.158 kb on + strand, within Pf1N1B4_540 at 638.158 kb on + strand, within Pf1N1B4_540 at 638.159 kb on - strand, within Pf1N1B4_540 at 638.159 kb on - strand at 638.159 kb on - strand, within Pf1N1B4_540 at 638.159 kb on - strand at 638.159 kb on - strand, within Pf1N1B4_540 at 638.159 kb on - strand, within Pf1N1B4_540 at 638.159 kb on - strand, within Pf1N1B4_540
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 635,361 + +0.4 635,500 + +0.9 635,500 + +2.1 635,518 - +0.2 635,760 + -2.1 635,760 + Pf1N1B4_537 0.28 +1.8 635,760 + Pf1N1B4_537 0.28 -1.0 635,761 - Pf1N1B4_537 0.28 -0.1 635,761 - Pf1N1B4_537 0.28 -1.4 635,761 - Pf1N1B4_537 0.28 +0.1 635,761 - Pf1N1B4_537 0.28 -0.8 635,761 - Pf1N1B4_537 0.28 -1.0 635,761 - Pf1N1B4_537 0.28 -0.4 635,761 - Pf1N1B4_537 0.28 -1.0 635,761 - Pf1N1B4_537 0.28 +1.7 635,761 - Pf1N1B4_537 0.28 -0.5 635,761 - Pf1N1B4_537 0.28 -0.6 635,761 - Pf1N1B4_537 0.28 +0.4 635,761 - Pf1N1B4_537 0.28 -1.2 635,761 - Pf1N1B4_537 0.28 +1.0 635,823 + Pf1N1B4_537 0.37 -2.3 635,823 + Pf1N1B4_537 0.37 +0.1 635,824 - Pf1N1B4_537 0.37 -1.1 635,824 - Pf1N1B4_537 0.37 -0.4 635,824 - Pf1N1B4_537 0.37 -1.8 635,834 + Pf1N1B4_537 0.38 +0.0 636,054 + Pf1N1B4_537 0.69 -1.1 636,054 + Pf1N1B4_537 0.69 +1.8 636,054 + Pf1N1B4_537 0.69 +0.6 636,055 - Pf1N1B4_537 0.69 -1.8 636,099 + Pf1N1B4_537 0.75 +1.4 636,099 + Pf1N1B4_537 0.75 -0.1 636,100 - Pf1N1B4_537 0.75 +0.5 636,100 - Pf1N1B4_537 0.75 +0.0 636,114 - Pf1N1B4_537 0.77 +0.1 636,171 + Pf1N1B4_537 0.85 -0.8 636,171 + Pf1N1B4_537 0.85 -0.1 636,172 - Pf1N1B4_537 0.85 -0.0 636,210 + -0.2 636,224 + -0.7 636,224 + +0.9 636,270 + +2.2 636,270 + -1.4 636,271 - -1.0 636,271 - +0.3 636,271 - +0.6 636,271 - +0.0 636,271 - -0.8 636,273 + -1.8 636,273 + -0.3 636,475 + Pf1N1B4_538 0.14 +0.4 636,476 - Pf1N1B4_538 0.14 -0.4 636,699 - -1.1 636,784 + Pf1N1B4_538 0.50 -1.2 637,219 + -0.5 637,219 + -0.5 637,219 + -2.0 637,219 + -1.0 637,219 + +0.4 637,220 - -0.0 637,226 + -0.4 637,232 + -0.9 637,233 - -0.6 637,233 - -0.8 637,494 - Pf1N1B4_539 0.39 +0.6 637,539 - Pf1N1B4_539 0.50 -0.6 637,638 - -2.2 637,649 + Pf1N1B4_539 0.78 -1.0 637,649 + Pf1N1B4_539 0.78 -0.3 637,650 - Pf1N1B4_539 0.78 +1.1 637,723 - -1.2 637,723 - +1.4 637,723 - +0.2 637,724 + +1.4 637,724 + +0.0 637,724 + -0.2 637,724 + -0.6 637,724 + -1.2 637,725 - +0.5 637,725 - +0.6 637,725 - +0.5 637,725 - -1.0 637,725 - -0.2 637,725 - -1.2 637,725 - +0.1 637,725 - -0.8 637,725 - -0.4 637,775 + -1.2 637,775 + -1.0 637,775 + -0.9 637,775 + +1.4 637,776 - +0.8 637,776 - -0.8 637,776 - +0.4 637,776 - +1.0 637,776 - -1.2 637,776 - -0.5 637,776 - +0.4 637,776 - -0.3 637,776 - +1.1 637,776 - +0.5 637,776 - -1.0 637,815 + -0.4 637,868 - +0.5 637,876 + +1.1 637,876 + -1.3 637,876 + -1.0 637,877 - -1.2 637,877 - +0.1 637,877 - -0.2 637,877 - -1.0 637,886 + +1.7 638,158 + Pf1N1B4_540 0.30 -2.5 638,158 + Pf1N1B4_540 0.30 +0.7 638,158 + Pf1N1B4_540 0.30 +1.0 638,159 - Pf1N1B4_540 0.30 +0.8 638,159 - -1.0 638,159 - Pf1N1B4_540 0.30 -0.3 638,159 - +1.4 638,159 - Pf1N1B4_540 0.30 +1.7 638,159 - Pf1N1B4_540 0.30 -1.0 638,159 - Pf1N1B4_540 0.30 -0.5
Or see this region's nucleotide sequence