Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5302

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5301 and Pf1N1B4_5302 are separated by 39 nucleotidesPf1N1B4_5302 and Pf1N1B4_5303 are separated by 359 nucleotidesPf1N1B4_5303 and Pf1N1B4_5304 are separated by 204 nucleotides Pf1N1B4_5301 - hypothetical protein, at 54,383 to 55,279 _5301 Pf1N1B4_5302 - hypothetical protein, at 55,319 to 55,537 _5302 Pf1N1B4_5303 - hypothetical protein, at 55,897 to 56,055 _5303 Pf1N1B4_5304 - hypothetical protein, at 56,260 to 56,787 _5304 Position (kb) 55 56Strain fitness (log2 ratio) -2 -1 0 1at 54.391 kb on - strandat 54.522 kb on + strand, within Pf1N1B4_5301at 54.559 kb on + strandat 54.565 kb on + strandat 54.565 kb on + strand, within Pf1N1B4_5301at 54.566 kb on - strand, within Pf1N1B4_5301at 54.566 kb on - strand, within Pf1N1B4_5301at 54.566 kb on - strand, within Pf1N1B4_5301at 54.593 kb on + strand, within Pf1N1B4_5301at 54.593 kb on + strand, within Pf1N1B4_5301at 54.593 kb on + strand, within Pf1N1B4_5301at 54.593 kb on + strand, within Pf1N1B4_5301at 54.594 kb on - strand, within Pf1N1B4_5301at 54.594 kb on - strand, within Pf1N1B4_5301at 54.594 kb on - strandat 54.594 kb on - strand, within Pf1N1B4_5301at 54.594 kb on - strand, within Pf1N1B4_5301at 54.594 kb on - strand, within Pf1N1B4_5301at 54.594 kb on - strand, within Pf1N1B4_5301at 54.619 kb on - strand, within Pf1N1B4_5301at 54.790 kb on + strand, within Pf1N1B4_5301at 54.859 kb on + strand, within Pf1N1B4_5301at 54.860 kb on - strand, within Pf1N1B4_5301at 54.976 kb on + strand, within Pf1N1B4_5301at 54.977 kb on - strand, within Pf1N1B4_5301at 55.030 kb on + strand, within Pf1N1B4_5301at 55.180 kb on + strand, within Pf1N1B4_5301at 55.180 kb on + strand, within Pf1N1B4_5301at 55.263 kb on + strandat 55.326 kb on - strandat 55.365 kb on + strand, within Pf1N1B4_5302at 55.365 kb on + strand, within Pf1N1B4_5302at 55.376 kb on + strand, within Pf1N1B4_5302at 55.407 kb on - strand, within Pf1N1B4_5302at 55.495 kb on + strand, within Pf1N1B4_5302at 55.495 kb on + strand, within Pf1N1B4_5302at 55.495 kb on + strand, within Pf1N1B4_5302at 55.505 kb on + strand, within Pf1N1B4_5302at 55.505 kb on + strand, within Pf1N1B4_5302at 55.555 kb on - strandat 55.586 kb on + strandat 55.587 kb on - strandat 55.613 kb on + strandat 55.722 kb on + strandat 55.722 kb on + strandat 55.722 kb on + strandat 55.722 kb on + strandat 55.722 kb on + strandat 55.722 kb on + strandat 55.722 kb on + strandat 55.723 kb on - strandat 55.723 kb on - strandat 55.723 kb on - strandat 55.723 kb on - strandat 55.723 kb on - strandat 55.723 kb on - strandat 55.817 kb on - strandat 55.837 kb on + strandat 55.837 kb on + strandat 55.837 kb on + strandat 55.853 kb on + strandat 55.887 kb on + strandat 55.911 kb on + strandat 56.053 kb on + strandat 56.053 kb on + strandat 56.053 kb on + strandat 56.054 kb on - strandat 56.089 kb on - strandat 56.147 kb on + strandat 56.147 kb on + strandat 56.148 kb on - strandat 56.428 kb on + strand, within Pf1N1B4_5304at 56.428 kb on + strand, within Pf1N1B4_5304at 56.429 kb on - strand, within Pf1N1B4_5304at 56.429 kb on - strand, within Pf1N1B4_5304at 56.429 kb on - strand, within Pf1N1B4_5304

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
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54,391 - -1.3
54,522 + Pf1N1B4_5301 0.15 -0.0
54,559 + -1.9
54,565 + -1.1
54,565 + Pf1N1B4_5301 0.20 -0.0
54,566 - Pf1N1B4_5301 0.20 +0.8
54,566 - Pf1N1B4_5301 0.20 -0.9
54,566 - Pf1N1B4_5301 0.20 +0.4
54,593 + Pf1N1B4_5301 0.23 -1.4
54,593 + Pf1N1B4_5301 0.23 +1.4
54,593 + Pf1N1B4_5301 0.23 -1.2
54,593 + Pf1N1B4_5301 0.23 +1.4
54,594 - Pf1N1B4_5301 0.24 -0.5
54,594 - Pf1N1B4_5301 0.24 -0.4
54,594 - +0.8
54,594 - Pf1N1B4_5301 0.24 +0.2
54,594 - Pf1N1B4_5301 0.24 -1.4
54,594 - Pf1N1B4_5301 0.24 -0.6
54,594 - Pf1N1B4_5301 0.24 +0.8
54,619 - Pf1N1B4_5301 0.26 -0.0
54,790 + Pf1N1B4_5301 0.45 -0.9
54,859 + Pf1N1B4_5301 0.53 +0.7
54,860 - Pf1N1B4_5301 0.53 -1.5
54,976 + Pf1N1B4_5301 0.66 -0.6
54,977 - Pf1N1B4_5301 0.66 +0.5
55,030 + Pf1N1B4_5301 0.72 -0.5
55,180 + Pf1N1B4_5301 0.89 +0.4
55,180 + Pf1N1B4_5301 0.89 +0.7
55,263 + -0.2
55,326 - -0.2
55,365 + Pf1N1B4_5302 0.21 -2.4
55,365 + Pf1N1B4_5302 0.21 -2.6
55,376 + Pf1N1B4_5302 0.26 -1.1
55,407 - Pf1N1B4_5302 0.40 -0.7
55,495 + Pf1N1B4_5302 0.80 -1.1
55,495 + Pf1N1B4_5302 0.80 +0.7
55,495 + Pf1N1B4_5302 0.80 +0.6
55,505 + Pf1N1B4_5302 0.85 -0.2
55,505 + Pf1N1B4_5302 0.85 -0.9
55,555 - +1.4
55,586 + -2.1
55,587 - -1.6
55,613 + -1.4
55,722 + -2.0
55,722 + +0.1
55,722 + +0.2
55,722 + -0.1
55,722 + +0.4
55,722 + -0.0
55,722 + +0.1
55,723 - -0.4
55,723 - -0.0
55,723 - +0.2
55,723 - +0.1
55,723 - +1.4
55,723 - -0.8
55,817 - +1.0
55,837 + +1.4
55,837 + -1.2
55,837 + -0.2
55,853 + +0.7
55,887 + -1.3
55,911 + +0.3
56,053 + +0.4
56,053 + +0.3
56,053 + +0.4
56,054 - -0.0
56,089 - +0.0
56,147 + -0.6
56,147 + -0.2
56,148 - -0.2
56,428 + Pf1N1B4_5304 0.32 -0.3
56,428 + Pf1N1B4_5304 0.32 -0.4
56,429 - Pf1N1B4_5304 0.32 +0.8
56,429 - Pf1N1B4_5304 0.32 -0.2
56,429 - Pf1N1B4_5304 0.32 -0.7

Or see this region's nucleotide sequence