Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5261

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
20,274	-		Pf1N1B4_5260	0.63	-1.2
20,277	+				+0.4
20,281	+		Pf1N1B4_5260	0.64	+1.1
20,285	-		Pf1N1B4_5260	0.64	-0.6
20,627	+				-1.9
20,627	+				-0.3
20,630	+				+0.7
20,657	+				-0.0
20,758	+				+1.4
20,977	+				+0.4
20,977	+				+0.0
21,099	+		Pf1N1B4_5261	0.62	-0.6
21,100	-		Pf1N1B4_5261	0.63	-1.2
21,100	-		Pf1N1B4_5261	0.63	+0.1
21,100	-		Pf1N1B4_5261	0.63	-2.6
21,200	+				+0.2
21,220	+				-1.4
21,359	+				+1.8
21,360	-				-0.7
21,589	+				-1.8
21,724	+		Pf1N1B4_5262	0.36	-0.7
21,725	-		Pf1N1B4_5262	0.37	-0.1
21,904	+		Pf1N1B4_5262	0.73	+0.6
21,904	+		Pf1N1B4_5262	0.73	-1.8
21,904	+		Pf1N1B4_5262	0.73	+0.8
21,905	-		Pf1N1B4_5262	0.73	+0.4
21,905	-		Pf1N1B4_5262	0.73	-0.3
21,905	-		Pf1N1B4_5262	0.73	-1.9
21,905	-		Pf1N1B4_5262	0.73	+0.8
21,941	+		Pf1N1B4_5262	0.81	-0.3
21,941	+		Pf1N1B4_5262	0.81	+0.1
21,943	+		Pf1N1B4_5262	0.81	-0.2
21,944	-		Pf1N1B4_5262	0.81	-0.6
21,944	-		Pf1N1B4_5262	0.81	+0.6
21,961	+		Pf1N1B4_5262	0.85	+0.8
22,078	-				-0.3
22,104	+				+0.2
22,105	-				-0.3
22,105	-				-2.5
22,159	+				-0.2
22,159	+				+0.7
22,160	-				-0.6
22,162	+				-1.4
22,162	+				-0.4
22,162	+				-0.6
22,162	+				+2.7
22,162	+				+0.4