Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4953

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
5,489,560	-		Pf1N1B4_4952	0.64	-1.2
5,489,779	-		Pf1N1B4_4952	0.80	+0.7
5,489,779	-		Pf1N1B4_4952	0.80	-1.0
5,490,191	-				+0.6
5,490,192	+				-0.3
5,490,192	+				-0.8
5,490,193	-				+0.7
5,490,196	+				+0.4
5,490,393	+		Pf1N1B4_4953	0.17	-0.1
5,491,182	-		Pf1N1B4_4953	0.58	-0.8
5,491,517	+		Pf1N1B4_4953	0.76	-0.9
5,491,518	-		Pf1N1B4_4953	0.76	-1.2
5,491,617	-		Pf1N1B4_4953	0.81	-0.6
5,491,898	-				+0.3
5,492,113	-				-0.1
5,492,116	+				-1.8
5,492,131	+				-0.8
5,492,354	+				-1.2
5,492,355	-				-0.3
5,492,416	-				-0.1
5,492,417	-				-2.0
5,492,451	-				-1.3
5,492,472	+				+0.1
5,492,473	-				+1.1
5,492,565	-				-0.2
5,492,593	+				+0.1
5,492,594	-				-0.6
5,492,627	-				-0.1
5,492,729	-				-1.6
5,492,729	-				-1.2
5,492,813	-				-0.8
5,492,853	+				-1.5
5,492,854	-				+0.1
5,492,854	-				+1.1
5,492,854	-				-1.1
5,492,854	-				-0.2
5,492,854	-				+0.6
5,492,880	-				-0.5
5,492,904	+				-0.3
5,492,905	-				+0.2
5,492,905	-				+0.1
5,492,961	+		Pf1N1B4_4954	0.11	-0.8
5,492,965	+		Pf1N1B4_4954	0.12	-0.6