Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_4943 and Pf1N1B4_4944 are separated by 6 nucleotides Pf1N1B4_4944 and Pf1N1B4_4945 are separated by 114 nucleotides Pf1N1B4_4945 and Pf1N1B4_4946 are separated by 78 nucleotides Pf1N1B4_4946 and Pf1N1B4_4947 are separated by 306 nucleotides 
        Pf1N1B4_4943 - Oxidoreductase, short chain dehydrogenase/reductase family, at 5,479,228 to 5,480,019 
        _4943 
         
        
        Pf1N1B4_4944 - hypothetical protein, at 5,480,026 to 5,480,151 
        _4944 
         
        
        Pf1N1B4_4945 - hypothetical protein, at 5,480,266 to 5,480,439 
        _4945 
         
        
        Pf1N1B4_4946 - putative aminotransferase, at 5,480,518 to 5,481,672 
        _4946 
         
        
        Pf1N1B4_4947 - Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing, at 5,481,979 to 5,485,044 
        _4947 
         Position (kb) 5480 
5481 
5482 Strain fitness (log2 ratio) -2 
-1 
0 
1 at 5479.985 kb on + strand at 5480.206 kb on + strand at 5480.206 kb on + strand at 5480.206 kb on + strand at 5480.224 kb on + strand at 5480.225 kb on - strand at 5480.282 kb on + strand at 5480.283 kb on - strand at 5480.332 kb on + strand, within Pf1N1B4_4945 at 5480.332 kb on + strand, within Pf1N1B4_4945 at 5480.333 kb on - strand, within Pf1N1B4_4945 at 5480.333 kb on - strand at 5480.469 kb on + strand at 5480.541 kb on + strand at 5480.542 kb on - strand at 5480.542 kb on - strand at 5480.542 kb on - strand at 5480.542 kb on - strand at 5480.542 kb on - strand at 5480.723 kb on + strand, within Pf1N1B4_4946 at 5480.978 kb on + strand, within Pf1N1B4_4946 at 5480.979 kb on - strand, within Pf1N1B4_4946 at 5480.979 kb on - strand, within Pf1N1B4_4946 at 5481.052 kb on + strand, within Pf1N1B4_4946 at 5481.053 kb on - strand, within Pf1N1B4_4946 at 5481.053 kb on - strand, within Pf1N1B4_4946 at 5481.245 kb on + strand at 5481.246 kb on - strand, within Pf1N1B4_4946 at 5481.446 kb on + strand, within Pf1N1B4_4946 at 5481.901 kb on + strand at 5482.165 kb on + strand at 5482.166 kb on - strand at 5482.166 kb on - strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 5,479,985 +  +0.2 5,480,206 +  +0.1 5,480,206 +  +0.8 5,480,206 +  -0.4 5,480,224 +  +0.6 5,480,225 -  +0.3 5,480,282 +  +0.9 5,480,283 -  +0.1 5,480,332 +  Pf1N1B4_4945 0.38  +0.3 5,480,332 +  Pf1N1B4_4945 0.38  -1.2 5,480,333 -  Pf1N1B4_4945 0.39  +0.3 5,480,333 -  -2.0 5,480,469 +  +0.3 5,480,541 +  -0.7 5,480,542 -  +0.0 5,480,542 -  -0.2 5,480,542 -  -1.2 5,480,542 -  -0.4 5,480,542 -  +0.4 5,480,723 +  Pf1N1B4_4946 0.18  -0.2 5,480,978 +  Pf1N1B4_4946 0.40  -0.8 5,480,979 -  Pf1N1B4_4946 0.40  -1.7 5,480,979 -  Pf1N1B4_4946 0.40  -0.7 5,481,052 +  Pf1N1B4_4946 0.46  -1.0 5,481,053 -  Pf1N1B4_4946 0.46  -0.5 5,481,053 -  Pf1N1B4_4946 0.46  -1.0 5,481,245 +  -0.7 5,481,246 -  Pf1N1B4_4946 0.63  +0.6 5,481,446 +  Pf1N1B4_4946 0.80  -1.3 5,481,901 +  -1.7 5,482,165 +  +0.4 5,482,166 -  -0.1 5,482,166 -  -0.3 
Or see this region's nucleotide sequence