Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4878 and Pf1N1B4_4879 are separated by 102 nucleotides Pf1N1B4_4879 and Pf1N1B4_4880 overlap by 14 nucleotides Pf1N1B4_4880 and Pf1N1B4_4881 are separated by 25 nucleotides
Pf1N1B4_4878 - NADH:flavin oxidoreductase/NADH oxidase, at 5,396,588 to 5,397,040
_4878
Pf1N1B4_4879 - Chromosome segregation ATPases, at 5,397,143 to 5,397,517
_4879
Pf1N1B4_4880 - Xanthine and CO dehydrogenases maturation factor, XdhC/CoxF family, at 5,397,504 to 5,398,472
_4880
Pf1N1B4_4881 - Hypothetical transcriptional regulator YqhC, at 5,398,498 to 5,399,409
_4881
Position (kb)
5397
5398
5399 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5396.891 kb on - strand, within Pf1N1B4_4878 at 5396.923 kb on + strand at 5396.924 kb on - strand, within Pf1N1B4_4878 at 5397.288 kb on + strand, within Pf1N1B4_4879 at 5397.288 kb on + strand, within Pf1N1B4_4879 at 5397.288 kb on + strand, within Pf1N1B4_4879 at 5397.289 kb on - strand at 5397.289 kb on - strand, within Pf1N1B4_4879 at 5397.440 kb on - strand at 5397.443 kb on + strand, within Pf1N1B4_4879 at 5397.631 kb on + strand, within Pf1N1B4_4880 at 5397.856 kb on + strand at 5397.926 kb on + strand at 5397.927 kb on - strand at 5397.927 kb on - strand, within Pf1N1B4_4880 at 5397.981 kb on - strand at 5398.231 kb on + strand, within Pf1N1B4_4880 at 5398.266 kb on + strand, within Pf1N1B4_4880 at 5398.267 kb on - strand, within Pf1N1B4_4880 at 5398.370 kb on + strand, within Pf1N1B4_4880 at 5398.371 kb on - strand, within Pf1N1B4_4880 at 5398.371 kb on - strand, within Pf1N1B4_4880 at 5398.468 kb on + strand at 5398.468 kb on + strand at 5398.468 kb on + strand at 5398.470 kb on + strand at 5398.470 kb on + strand at 5398.471 kb on - strand at 5398.653 kb on + strand, within Pf1N1B4_4881 at 5398.653 kb on + strand, within Pf1N1B4_4881 at 5398.653 kb on + strand, within Pf1N1B4_4881 at 5398.653 kb on + strand, within Pf1N1B4_4881 at 5398.654 kb on - strand, within Pf1N1B4_4881 at 5398.657 kb on + strand, within Pf1N1B4_4881 at 5398.722 kb on + strand, within Pf1N1B4_4881 at 5398.824 kb on + strand, within Pf1N1B4_4881 at 5398.825 kb on - strand, within Pf1N1B4_4881 at 5398.847 kb on + strand at 5398.847 kb on + strand, within Pf1N1B4_4881 at 5398.982 kb on + strand, within Pf1N1B4_4881 at 5398.983 kb on - strand at 5398.983 kb on - strand, within Pf1N1B4_4881 at 5399.060 kb on + strand, within Pf1N1B4_4881 at 5399.060 kb on + strand, within Pf1N1B4_4881 at 5399.060 kb on + strand, within Pf1N1B4_4881 at 5399.060 kb on + strand, within Pf1N1B4_4881 at 5399.061 kb on - strand, within Pf1N1B4_4881 at 5399.097 kb on - strand, within Pf1N1B4_4881 at 5399.276 kb on + strand, within Pf1N1B4_4881 at 5399.276 kb on + strand, within Pf1N1B4_4881 at 5399.276 kb on + strand, within Pf1N1B4_4881 at 5399.277 kb on - strand, within Pf1N1B4_4881 at 5399.320 kb on + strand at 5399.352 kb on + strand at 5399.352 kb on + strand at 5399.353 kb on - strand at 5399.387 kb on + strand at 5399.387 kb on + strand at 5399.388 kb on - strand at 5399.388 kb on - strand at 5399.411 kb on + strand at 5399.411 kb on + strand at 5399.411 kb on + strand at 5399.416 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 5,396,891 - Pf1N1B4_4878 0.67 +0.6 5,396,923 + -0.6 5,396,924 - Pf1N1B4_4878 0.74 +0.2 5,397,288 + Pf1N1B4_4879 0.39 -0.8 5,397,288 + Pf1N1B4_4879 0.39 +0.7 5,397,288 + Pf1N1B4_4879 0.39 -2.4 5,397,289 - -1.4 5,397,289 - Pf1N1B4_4879 0.39 -0.2 5,397,440 - -1.2 5,397,443 + Pf1N1B4_4879 0.80 +0.8 5,397,631 + Pf1N1B4_4880 0.13 -1.5 5,397,856 + -1.6 5,397,926 + +0.4 5,397,927 - +0.4 5,397,927 - Pf1N1B4_4880 0.44 -0.1 5,397,981 - +0.9 5,398,231 + Pf1N1B4_4880 0.75 -1.0 5,398,266 + Pf1N1B4_4880 0.79 -1.1 5,398,267 - Pf1N1B4_4880 0.79 -0.3 5,398,370 + Pf1N1B4_4880 0.89 -0.7 5,398,371 - Pf1N1B4_4880 0.89 -1.6 5,398,371 - Pf1N1B4_4880 0.89 -0.3 5,398,468 + +0.2 5,398,468 + -1.8 5,398,468 + -0.1 5,398,470 + -1.3 5,398,470 + -0.2 5,398,471 - -0.7 5,398,653 + Pf1N1B4_4881 0.17 -1.6 5,398,653 + Pf1N1B4_4881 0.17 -0.4 5,398,653 + Pf1N1B4_4881 0.17 -0.4 5,398,653 + Pf1N1B4_4881 0.17 +0.6 5,398,654 - Pf1N1B4_4881 0.17 -0.5 5,398,657 + Pf1N1B4_4881 0.17 -0.8 5,398,722 + Pf1N1B4_4881 0.25 +0.3 5,398,824 + Pf1N1B4_4881 0.36 -0.5 5,398,825 - Pf1N1B4_4881 0.36 +1.4 5,398,847 + +0.4 5,398,847 + Pf1N1B4_4881 0.38 +0.0 5,398,982 + Pf1N1B4_4881 0.53 -0.3 5,398,983 - -3.2 5,398,983 - Pf1N1B4_4881 0.53 +0.2 5,399,060 + Pf1N1B4_4881 0.62 -0.1 5,399,060 + Pf1N1B4_4881 0.62 -0.5 5,399,060 + Pf1N1B4_4881 0.62 +0.2 5,399,060 + Pf1N1B4_4881 0.62 +1.1 5,399,061 - Pf1N1B4_4881 0.62 -1.3 5,399,097 - Pf1N1B4_4881 0.66 -0.2 5,399,276 + Pf1N1B4_4881 0.85 +0.9 5,399,276 + Pf1N1B4_4881 0.85 +0.9 5,399,276 + Pf1N1B4_4881 0.85 -0.5 5,399,277 - Pf1N1B4_4881 0.85 +0.1 5,399,320 + -2.0 5,399,352 + -0.1 5,399,352 + -1.0 5,399,353 - -0.0 5,399,387 + -0.3 5,399,387 + -0.0 5,399,388 - -1.6 5,399,388 - +0.5 5,399,411 + -2.0 5,399,411 + +0.7 5,399,411 + -0.4 5,399,416 + +0.9
Or see this region's nucleotide sequence