Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4845 and Pf1N1B4_4846 are separated by 126 nucleotides Pf1N1B4_4846 and Pf1N1B4_4847 are separated by 14 nucleotides
Pf1N1B4_4845 - Xylulose kinase (EC 2.7.1.17), at 5,352,753 to 5,354,249
_4845
Pf1N1B4_4846 - Multiple polyol-specific dehydrogenase (EC 1.1.1.-), at 5,354,376 to 5,355,851
_4846
Pf1N1B4_4847 - Various polyols ABC transporter, ATP-binding component, at 5,355,866 to 5,356,969
_4847
Position (kb) 5354
5355
5356 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5353.426 kb on + strand, within Pf1N1B4_4845 at 5353.427 kb on - strand at 5353.473 kb on + strand, within Pf1N1B4_4845 at 5353.473 kb on + strand at 5353.474 kb on - strand, within Pf1N1B4_4845 at 5353.475 kb on + strand, within Pf1N1B4_4845 at 5353.714 kb on + strand, within Pf1N1B4_4845 at 5353.714 kb on + strand, within Pf1N1B4_4845 at 5353.744 kb on + strand, within Pf1N1B4_4845 at 5353.744 kb on + strand, within Pf1N1B4_4845 at 5353.745 kb on - strand at 5353.745 kb on - strand, within Pf1N1B4_4845 at 5353.753 kb on + strand, within Pf1N1B4_4845 at 5354.037 kb on + strand, within Pf1N1B4_4845 at 5354.038 kb on - strand, within Pf1N1B4_4845 at 5354.232 kb on + strand at 5354.232 kb on + strand at 5354.232 kb on + strand at 5354.233 kb on - strand at 5354.233 kb on - strand at 5354.261 kb on - strand at 5354.581 kb on + strand, within Pf1N1B4_4846 at 5354.582 kb on - strand, within Pf1N1B4_4846 at 5354.582 kb on - strand, within Pf1N1B4_4846 at 5354.582 kb on - strand, within Pf1N1B4_4846 at 5354.792 kb on - strand, within Pf1N1B4_4846 at 5354.851 kb on + strand, within Pf1N1B4_4846 at 5354.863 kb on + strand, within Pf1N1B4_4846 at 5354.864 kb on - strand, within Pf1N1B4_4846 at 5354.929 kb on + strand at 5354.930 kb on - strand, within Pf1N1B4_4846 at 5355.610 kb on + strand, within Pf1N1B4_4846 at 5355.610 kb on + strand, within Pf1N1B4_4846 at 5355.718 kb on + strand at 5355.719 kb on - strand at 5355.794 kb on - strand at 5356.404 kb on + strand, within Pf1N1B4_4847 at 5356.404 kb on + strand, within Pf1N1B4_4847 at 5356.404 kb on + strand, within Pf1N1B4_4847 at 5356.404 kb on + strand, within Pf1N1B4_4847 at 5356.405 kb on - strand, within Pf1N1B4_4847 at 5356.720 kb on - strand, within Pf1N1B4_4847 at 5356.720 kb on - strand, within Pf1N1B4_4847
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 5,353,426 + Pf1N1B4_4845 0.45 +0.0 5,353,427 - +0.4 5,353,473 + Pf1N1B4_4845 0.48 +0.5 5,353,473 + +0.4 5,353,474 - Pf1N1B4_4845 0.48 -0.4 5,353,475 + Pf1N1B4_4845 0.48 -0.8 5,353,714 + Pf1N1B4_4845 0.64 +0.4 5,353,714 + Pf1N1B4_4845 0.64 -0.6 5,353,744 + Pf1N1B4_4845 0.66 +0.0 5,353,744 + Pf1N1B4_4845 0.66 +0.4 5,353,745 - +0.0 5,353,745 - Pf1N1B4_4845 0.66 -0.2 5,353,753 + Pf1N1B4_4845 0.67 +1.0 5,354,037 + Pf1N1B4_4845 0.86 -1.2 5,354,038 - Pf1N1B4_4845 0.86 -1.4 5,354,232 + +0.5 5,354,232 + +1.4 5,354,232 + -0.6 5,354,233 - +1.6 5,354,233 - +1.2 5,354,261 - -0.2 5,354,581 + Pf1N1B4_4846 0.14 +0.2 5,354,582 - Pf1N1B4_4846 0.14 -0.4 5,354,582 - Pf1N1B4_4846 0.14 -1.2 5,354,582 - Pf1N1B4_4846 0.14 -2.1 5,354,792 - Pf1N1B4_4846 0.28 -0.4 5,354,851 + Pf1N1B4_4846 0.32 -1.8 5,354,863 + Pf1N1B4_4846 0.33 -1.0 5,354,864 - Pf1N1B4_4846 0.33 -3.4 5,354,929 + -1.1 5,354,930 - Pf1N1B4_4846 0.38 -0.2 5,355,610 + Pf1N1B4_4846 0.84 -0.7 5,355,610 + Pf1N1B4_4846 0.84 +0.1 5,355,718 + +0.4 5,355,719 - -0.2 5,355,794 - -0.2 5,356,404 + Pf1N1B4_4847 0.49 +1.8 5,356,404 + Pf1N1B4_4847 0.49 +1.0 5,356,404 + Pf1N1B4_4847 0.49 +0.4 5,356,404 + Pf1N1B4_4847 0.49 -1.0 5,356,405 - Pf1N1B4_4847 0.49 +0.0 5,356,720 - Pf1N1B4_4847 0.77 -0.3 5,356,720 - Pf1N1B4_4847 0.77 -0.2
Or see this region's nucleotide sequence