Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4473

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
4,933,529	-				-0.2
4,933,537	+				-2.7
4,933,538	-				+0.3
4,933,610	+				+1.3
4,933,613	+				-0.5
4,933,613	+				-1.1
4,933,614	-				-0.6
4,933,614	-				-0.1
4,933,655	+				-1.0
4,933,655	+				-1.1
4,933,655	+				-0.1
4,933,656	-				-0.8
4,933,656	-				-1.3
4,933,878	+				+0.3
4,933,878	+		Pf1N1B4_4472	0.29	-0.1
4,933,878	+		Pf1N1B4_4472	0.29	-0.4
4,933,878	+		Pf1N1B4_4472	0.29	+0.1
4,933,879	-				-1.8
4,934,541	+		Pf1N1B4_4473	0.20	-1.6
4,934,541	+		Pf1N1B4_4473	0.20	-3.0
4,934,542	-				+1.3
4,934,542	-				+0.3
4,934,604	+				+0.7
4,934,605	-		Pf1N1B4_4473	0.31	-1.0
4,934,770	-		Pf1N1B4_4473	0.58	-0.3
4,934,802	+		Pf1N1B4_4473	0.63	-0.6
4,934,803	-		Pf1N1B4_4473	0.63	+0.3
4,935,002	+				-0.3
4,935,002	+				-1.5
4,935,002	+				+0.7
4,935,098	+				+0.9
4,935,102	+				+0.3
4,935,111	+				-1.0
4,935,155	-				+0.2
4,935,339	-		Pf1N1B4_4474	0.22	+0.2
4,935,339	-		Pf1N1B4_4474	0.22	-0.3
4,935,627	+		Pf1N1B4_4474	0.54	-0.4
4,935,628	-				+0.3
4,935,628	-		Pf1N1B4_4474	0.54	-1.4
4,935,935	+		Pf1N1B4_4474	0.87	-0.7