Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4433

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
4,893,761	-				-0.0
4,893,823	-				-0.0
4,893,896	+				+0.0
4,893,896	+				+1.6
4,893,897	-				-1.0
4,893,897	-				+0.3
4,893,925	-				+0.1
4,893,952	+				+1.3
4,893,952	+				-0.5
4,893,953	-				-0.1
4,893,953	-				+0.7
4,893,987	+				+0.1
4,893,987	+				+1.3
4,893,988	-				+0.1
4,894,233	+		Pf1N1B4_4432	0.44	-0.5
4,894,467	+		Pf1N1B4_4432	0.78	-1.1
4,894,467	+		Pf1N1B4_4432	0.78	+0.0
4,894,468	-		Pf1N1B4_4432	0.78	-0.9
4,894,468	-		Pf1N1B4_4432	0.78	-0.1
4,894,634	-				+0.9
4,894,634	-				-0.7
4,894,634	-				-0.7
4,894,637	+				+0.6
4,894,637	+				+0.6
4,894,637	+				+1.3
4,894,637	+				-0.1
4,894,637	+				+0.1
4,894,638	-				-0.5
4,894,638	-				+0.6
4,894,767	-		Pf1N1B4_4433	0.19	-1.3
4,894,767	-		Pf1N1B4_4433	0.19	-0.4
4,894,801	-		Pf1N1B4_4433	0.26	-0.8
4,895,178	+				-0.4
4,895,178	+				-0.3
4,895,179	-				-0.9
4,895,179	-				+0.7
4,895,429	-				+2.3
4,895,429	-		Pf1N1B4_4435	0.17	-1.6
4,895,429	-		Pf1N1B4_4435	0.17	+0.4
4,895,429	-		Pf1N1B4_4435	0.17	-0.8
4,895,429	-		Pf1N1B4_4435	0.17	-0.0
4,895,653	+		Pf1N1B4_4435	0.53	-2.6
4,895,659	+		Pf1N1B4_4435	0.53	+0.3
4,895,660	-		Pf1N1B4_4435	0.54	-0.3
4,895,660	-				-0.9
4,895,660	-		Pf1N1B4_4435	0.54	-1.5
4,895,660	-		Pf1N1B4_4435	0.54	+0.1
4,896,087	+				-1.5
4,896,087	+				-0.0