Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_435 and Pf1N1B4_436 are separated by 524 nucleotides Pf1N1B4_436 and Pf1N1B4_437 are separated by 264 nucleotides
Pf1N1B4_435 - hypothetical protein APECO1_2271, at 513,765 to 514,730
_435
Pf1N1B4_436 - Cell division protein FtsH (EC 3.4.24.-), at 515,255 to 516,193
_436
Pf1N1B4_437 - hypothetical protein, at 516,458 to 516,589
_437
Position (kb) 515
516
517 Strain fitness (log2 ratio) -2
-1
0
1
2 at 514.271 kb on + strand, within Pf1N1B4_435 at 514.306 kb on + strand, within Pf1N1B4_435 at 514.441 kb on + strand, within Pf1N1B4_435 at 514.454 kb on + strand, within Pf1N1B4_435 at 514.455 kb on - strand, within Pf1N1B4_435 at 514.455 kb on - strand, within Pf1N1B4_435 at 514.455 kb on - strand, within Pf1N1B4_435 at 514.458 kb on + strand at 514.458 kb on + strand, within Pf1N1B4_435 at 514.458 kb on + strand, within Pf1N1B4_435 at 514.458 kb on + strand, within Pf1N1B4_435 at 514.458 kb on + strand, within Pf1N1B4_435 at 514.458 kb on + strand, within Pf1N1B4_435 at 514.459 kb on - strand, within Pf1N1B4_435 at 514.459 kb on - strand, within Pf1N1B4_435 at 514.459 kb on - strand, within Pf1N1B4_435 at 514.459 kb on - strand, within Pf1N1B4_435 at 514.502 kb on + strand, within Pf1N1B4_435 at 514.502 kb on + strand, within Pf1N1B4_435 at 514.503 kb on - strand, within Pf1N1B4_435 at 514.503 kb on - strand, within Pf1N1B4_435 at 514.506 kb on + strand, within Pf1N1B4_435 at 514.506 kb on + strand, within Pf1N1B4_435 at 514.506 kb on + strand, within Pf1N1B4_435 at 514.506 kb on + strand, within Pf1N1B4_435 at 514.506 kb on + strand at 514.506 kb on + strand, within Pf1N1B4_435 at 514.506 kb on + strand, within Pf1N1B4_435 at 514.506 kb on + strand, within Pf1N1B4_435 at 514.506 kb on + strand, within Pf1N1B4_435 at 514.506 kb on + strand, within Pf1N1B4_435 at 514.507 kb on - strand, within Pf1N1B4_435 at 514.507 kb on - strand, within Pf1N1B4_435 at 514.507 kb on - strand, within Pf1N1B4_435 at 514.507 kb on - strand, within Pf1N1B4_435 at 514.507 kb on - strand, within Pf1N1B4_435 at 514.507 kb on - strand, within Pf1N1B4_435 at 514.507 kb on - strand, within Pf1N1B4_435 at 514.507 kb on - strand, within Pf1N1B4_435 at 514.528 kb on + strand, within Pf1N1B4_435 at 514.528 kb on + strand, within Pf1N1B4_435 at 514.528 kb on + strand, within Pf1N1B4_435 at 514.528 kb on + strand, within Pf1N1B4_435 at 514.528 kb on + strand, within Pf1N1B4_435 at 514.529 kb on - strand, within Pf1N1B4_435 at 514.529 kb on - strand, within Pf1N1B4_435 at 514.579 kb on + strand, within Pf1N1B4_435 at 514.579 kb on + strand, within Pf1N1B4_435 at 514.579 kb on + strand, within Pf1N1B4_435 at 514.579 kb on + strand, within Pf1N1B4_435 at 514.580 kb on - strand at 514.580 kb on - strand, within Pf1N1B4_435 at 514.580 kb on - strand at 514.662 kb on + strand at 514.662 kb on + strand at 514.663 kb on - strand at 514.663 kb on - strand at 514.681 kb on + strand at 514.681 kb on + strand at 514.681 kb on + strand at 514.681 kb on + strand at 514.681 kb on + strand at 514.681 kb on + strand at 514.681 kb on + strand at 514.682 kb on - strand at 514.739 kb on + strand at 514.739 kb on + strand at 514.739 kb on + strand at 514.739 kb on + strand at 514.739 kb on + strand at 514.739 kb on + strand at 514.739 kb on + strand at 514.739 kb on + strand at 514.740 kb on - strand at 514.740 kb on - strand at 514.740 kb on - strand at 514.740 kb on - strand at 514.740 kb on - strand at 514.740 kb on - strand at 514.740 kb on - strand at 514.749 kb on + strand at 514.749 kb on + strand at 514.750 kb on - strand at 514.750 kb on - strand at 514.755 kb on + strand at 514.755 kb on + strand at 514.755 kb on + strand at 514.756 kb on - strand at 514.756 kb on - strand at 514.756 kb on - strand at 514.757 kb on + strand at 514.757 kb on + strand at 514.757 kb on + strand at 514.758 kb on - strand at 514.758 kb on - strand at 514.781 kb on + strand at 514.782 kb on - strand at 514.782 kb on - strand at 514.802 kb on + strand at 514.802 kb on + strand at 514.802 kb on + strand at 514.802 kb on + strand at 514.803 kb on - strand at 514.894 kb on + strand at 514.980 kb on + strand at 514.980 kb on + strand at 515.049 kb on - strand at 515.067 kb on + strand at 515.073 kb on + strand at 515.073 kb on + strand at 515.073 kb on + strand at 515.082 kb on + strand at 515.083 kb on - strand at 515.083 kb on - strand at 515.083 kb on - strand at 515.083 kb on - strand at 515.084 kb on + strand at 515.084 kb on + strand at 515.084 kb on + strand at 515.085 kb on - strand at 515.156 kb on + strand at 515.205 kb on + strand at 515.205 kb on + strand at 515.205 kb on + strand at 515.205 kb on + strand at 515.206 kb on - strand at 515.242 kb on + strand at 515.254 kb on - strand at 515.254 kb on - strand at 515.254 kb on - strand at 515.260 kb on + strand at 515.260 kb on + strand at 515.261 kb on - strand at 515.264 kb on - strand at 515.264 kb on - strand at 515.269 kb on + strand at 515.269 kb on + strand at 515.270 kb on - strand at 515.270 kb on - strand at 515.270 kb on - strand at 515.270 kb on - strand at 515.270 kb on - strand at 515.304 kb on - strand at 515.343 kb on + strand at 515.343 kb on + strand at 515.343 kb on + strand at 515.343 kb on + strand at 515.344 kb on - strand at 515.344 kb on - strand at 515.344 kb on - strand at 515.344 kb on - strand at 515.389 kb on - strand, within Pf1N1B4_436 at 515.437 kb on + strand, within Pf1N1B4_436 at 515.464 kb on - strand, within Pf1N1B4_436 at 515.464 kb on - strand, within Pf1N1B4_436 at 515.466 kb on + strand, within Pf1N1B4_436 at 515.467 kb on - strand at 515.635 kb on + strand, within Pf1N1B4_436 at 515.654 kb on + strand, within Pf1N1B4_436 at 515.655 kb on - strand, within Pf1N1B4_436 at 515.892 kb on + strand, within Pf1N1B4_436 at 515.892 kb on + strand, within Pf1N1B4_436 at 515.893 kb on - strand, within Pf1N1B4_436 at 515.917 kb on + strand, within Pf1N1B4_436 at 515.917 kb on + strand, within Pf1N1B4_436 at 515.917 kb on + strand, within Pf1N1B4_436 at 515.917 kb on + strand, within Pf1N1B4_436 at 515.918 kb on - strand, within Pf1N1B4_436 at 515.918 kb on - strand, within Pf1N1B4_436 at 515.918 kb on - strand, within Pf1N1B4_436 at 515.918 kb on - strand, within Pf1N1B4_436 at 515.951 kb on + strand, within Pf1N1B4_436 at 515.975 kb on - strand, within Pf1N1B4_436 at 516.029 kb on - strand, within Pf1N1B4_436 at 516.029 kb on - strand, within Pf1N1B4_436 at 516.029 kb on - strand, within Pf1N1B4_436 at 516.090 kb on + strand, within Pf1N1B4_436 at 516.090 kb on + strand, within Pf1N1B4_436 at 516.129 kb on + strand at 516.242 kb on - strand at 516.315 kb on + strand at 516.316 kb on - strand at 516.410 kb on + strand at 516.411 kb on - strand at 516.415 kb on - strand at 516.514 kb on + strand, within Pf1N1B4_437 at 516.514 kb on + strand, within Pf1N1B4_437 at 516.542 kb on + strand, within Pf1N1B4_437 at 516.542 kb on + strand, within Pf1N1B4_437 at 516.543 kb on - strand, within Pf1N1B4_437 at 516.543 kb on - strand, within Pf1N1B4_437 at 516.543 kb on - strand, within Pf1N1B4_437 at 516.563 kb on + strand, within Pf1N1B4_437 at 516.564 kb on - strand, within Pf1N1B4_437 at 516.564 kb on - strand, within Pf1N1B4_437 at 516.564 kb on - strand, within Pf1N1B4_437 at 516.564 kb on - strand, within Pf1N1B4_437 at 516.602 kb on + strand at 516.602 kb on + strand at 516.603 kb on - strand at 516.603 kb on - strand at 516.603 kb on - strand at 516.603 kb on - strand at 516.604 kb on + strand at 516.794 kb on - strand at 516.897 kb on + strand at 516.959 kb on + strand at 517.077 kb on + strand at 517.079 kb on + strand at 517.079 kb on + strand at 517.183 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 514,271 + Pf1N1B4_435 0.52 +0.8 514,306 + Pf1N1B4_435 0.56 +0.6 514,441 + Pf1N1B4_435 0.70 +0.8 514,454 + Pf1N1B4_435 0.71 +0.0 514,455 - Pf1N1B4_435 0.71 -1.6 514,455 - Pf1N1B4_435 0.71 +2.6 514,455 - Pf1N1B4_435 0.71 +0.3 514,458 + +0.9 514,458 + Pf1N1B4_435 0.72 +1.0 514,458 + Pf1N1B4_435 0.72 -1.3 514,458 + Pf1N1B4_435 0.72 +1.6 514,458 + Pf1N1B4_435 0.72 -2.7 514,458 + Pf1N1B4_435 0.72 -0.2 514,459 - Pf1N1B4_435 0.72 -0.8 514,459 - Pf1N1B4_435 0.72 +0.0 514,459 - Pf1N1B4_435 0.72 -0.2 514,459 - Pf1N1B4_435 0.72 -0.1 514,502 + Pf1N1B4_435 0.76 +0.2 514,502 + Pf1N1B4_435 0.76 +0.6 514,503 - Pf1N1B4_435 0.76 -1.2 514,503 - Pf1N1B4_435 0.76 -0.0 514,506 + Pf1N1B4_435 0.77 -0.5 514,506 + Pf1N1B4_435 0.77 +2.4 514,506 + Pf1N1B4_435 0.77 +0.5 514,506 + Pf1N1B4_435 0.77 -1.3 514,506 + -1.0 514,506 + Pf1N1B4_435 0.77 -0.2 514,506 + Pf1N1B4_435 0.77 -0.0 514,506 + Pf1N1B4_435 0.77 -0.2 514,506 + Pf1N1B4_435 0.77 +0.2 514,506 + Pf1N1B4_435 0.77 +2.4 514,507 - Pf1N1B4_435 0.77 +0.9 514,507 - Pf1N1B4_435 0.77 +0.7 514,507 - Pf1N1B4_435 0.77 +0.0 514,507 - Pf1N1B4_435 0.77 +2.2 514,507 - Pf1N1B4_435 0.77 +0.8 514,507 - Pf1N1B4_435 0.77 +1.0 514,507 - Pf1N1B4_435 0.77 +0.0 514,507 - Pf1N1B4_435 0.77 -0.4 514,528 + Pf1N1B4_435 0.79 +0.6 514,528 + Pf1N1B4_435 0.79 -1.3 514,528 + Pf1N1B4_435 0.79 +0.2 514,528 + Pf1N1B4_435 0.79 +0.7 514,528 + Pf1N1B4_435 0.79 +0.2 514,529 - Pf1N1B4_435 0.79 +1.7 514,529 - Pf1N1B4_435 0.79 +0.7 514,579 + Pf1N1B4_435 0.84 -0.1 514,579 + Pf1N1B4_435 0.84 +1.0 514,579 + Pf1N1B4_435 0.84 +0.7 514,579 + Pf1N1B4_435 0.84 -1.6 514,580 - +1.4 514,580 - Pf1N1B4_435 0.84 -1.0 514,580 - -1.2 514,662 + +0.4 514,662 + -1.4 514,663 - +0.9 514,663 - +0.5 514,681 + -0.3 514,681 + +0.5 514,681 + -0.0 514,681 + -1.0 514,681 + -0.2 514,681 + +1.0 514,681 + -0.8 514,682 - -0.7 514,739 + +1.4 514,739 + -1.9 514,739 + +0.4 514,739 + -0.6 514,739 + -0.2 514,739 + -0.6 514,739 + +1.7 514,739 + +0.4 514,740 - -0.4 514,740 - -1.4 514,740 - -1.4 514,740 - +0.6 514,740 - -0.6 514,740 - -0.9 514,740 - +0.5 514,749 + +0.4 514,749 + -0.8 514,750 - +2.4 514,750 - +0.5 514,755 + -1.0 514,755 + +0.0 514,755 + -1.2 514,756 - -0.7 514,756 - -0.5 514,756 - +2.0 514,757 + +0.2 514,757 + +0.6 514,757 + -1.5 514,758 - -0.6 514,758 - -1.2 514,781 + -1.4 514,782 - -1.2 514,782 - +0.8 514,802 + +0.0 514,802 + +0.4 514,802 + -0.2 514,802 + -1.8 514,803 - -0.6 514,894 + -1.6 514,980 + +1.0 514,980 + -0.0 515,049 - +0.8 515,067 + -1.7 515,073 + -0.2 515,073 + -0.3 515,073 + -1.2 515,082 + +0.9 515,083 - +1.1 515,083 - -1.0 515,083 - -0.1 515,083 - +0.4 515,084 + -1.3 515,084 + +0.9 515,084 + -0.1 515,085 - +0.0 515,156 + +0.2 515,205 + -0.6 515,205 + -2.0 515,205 + -0.4 515,205 + +0.5 515,206 - -0.6 515,242 + -0.5 515,254 - +0.8 515,254 - -0.0 515,254 - -0.8 515,260 + -1.2 515,260 + +1.4 515,261 - +1.6 515,264 - +0.9 515,264 - +0.8 515,269 + +0.9 515,269 + +1.4 515,270 - +0.8 515,270 - +2.5 515,270 - -0.6 515,270 - +2.0 515,270 - -0.1 515,304 - -0.7 515,343 + -0.7 515,343 + -0.4 515,343 + -0.6 515,343 + -0.4 515,344 - -0.2 515,344 - -0.1 515,344 - -1.0 515,344 - +1.4 515,389 - Pf1N1B4_436 0.14 +1.0 515,437 + Pf1N1B4_436 0.19 -0.3 515,464 - Pf1N1B4_436 0.22 +0.1 515,464 - Pf1N1B4_436 0.22 +0.7 515,466 + Pf1N1B4_436 0.22 -0.0 515,467 - -0.2 515,635 + Pf1N1B4_436 0.40 +0.0 515,654 + Pf1N1B4_436 0.42 -0.2 515,655 - Pf1N1B4_436 0.43 +0.1 515,892 + Pf1N1B4_436 0.68 -0.3 515,892 + Pf1N1B4_436 0.68 +1.0 515,893 - Pf1N1B4_436 0.68 -0.8 515,917 + Pf1N1B4_436 0.71 +1.0 515,917 + Pf1N1B4_436 0.71 +0.6 515,917 + Pf1N1B4_436 0.71 +0.8 515,917 + Pf1N1B4_436 0.71 -0.3 515,918 - Pf1N1B4_436 0.71 +0.4 515,918 - Pf1N1B4_436 0.71 +0.4 515,918 - Pf1N1B4_436 0.71 +0.7 515,918 - Pf1N1B4_436 0.71 -0.3 515,951 + Pf1N1B4_436 0.74 -0.4 515,975 - Pf1N1B4_436 0.77 -0.0 516,029 - Pf1N1B4_436 0.82 +0.8 516,029 - Pf1N1B4_436 0.82 +0.6 516,029 - Pf1N1B4_436 0.82 +0.7 516,090 + Pf1N1B4_436 0.89 +0.0 516,090 + Pf1N1B4_436 0.89 +0.6 516,129 + -0.3 516,242 - -0.8 516,315 + +0.6 516,316 - -0.4 516,410 + +0.6 516,411 - +0.8 516,415 - -0.2 516,514 + Pf1N1B4_437 0.42 +1.0 516,514 + Pf1N1B4_437 0.42 -1.6 516,542 + Pf1N1B4_437 0.64 -1.0 516,542 + Pf1N1B4_437 0.64 +0.1 516,543 - Pf1N1B4_437 0.64 -0.7 516,543 - Pf1N1B4_437 0.64 +0.4 516,543 - Pf1N1B4_437 0.64 -0.6 516,563 + Pf1N1B4_437 0.80 +0.5 516,564 - Pf1N1B4_437 0.80 +0.5 516,564 - Pf1N1B4_437 0.80 -0.5 516,564 - Pf1N1B4_437 0.80 +0.1 516,564 - Pf1N1B4_437 0.80 -0.1 516,602 + +0.5 516,602 + -0.1 516,603 - -0.6 516,603 - +0.6 516,603 - -0.6 516,603 - +1.1 516,604 + -2.0 516,794 - -0.2 516,897 + +1.4 516,959 + +0.5 517,077 + -1.3 517,079 + -0.2 517,079 + +0.4 517,183 - +0.4
Or see this region's nucleotide sequence