Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4212

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
4,665,472	+				-1.3
4,665,555	+				+0.2
4,665,636	+				-0.3
4,665,636	+				+0.9
4,665,636	+				+0.2
4,665,636	+				+0.3
4,665,637	-				+0.9
4,665,637	-				-1.0
4,665,637	-				-0.7
4,665,893	+				-0.7
4,666,349	+				+0.3
4,666,437	+				-2.0
4,666,462	-				+1.3
4,666,492	+				-1.5
4,666,492	+				-0.7
4,666,492	+				-0.8
4,666,493	-				-1.3
4,666,503	+				-0.6
4,666,525	+				+1.1
4,666,730	+		Pf1N1B4_4212	0.61	-0.5
4,666,730	+		Pf1N1B4_4212	0.61	+1.5
4,666,730	+		Pf1N1B4_4212	0.61	-2.7
4,666,730	+		Pf1N1B4_4212	0.61	-1.0
4,666,733	+		Pf1N1B4_4212	0.61	-1.1
4,666,893	+				-0.4
4,667,049	-		Pf1N1B4_4213	0.12	+0.2
4,667,049	-		Pf1N1B4_4213	0.12	-0.5
4,667,094	+		Pf1N1B4_4213	0.17	-2.9
4,667,094	+		Pf1N1B4_4213	0.17	+2.1
4,667,188	-		Pf1N1B4_4213	0.28	-1.2
4,667,188	-		Pf1N1B4_4213	0.28	+0.0
4,667,194	-		Pf1N1B4_4213	0.29	+0.5
4,667,197	+		Pf1N1B4_4213	0.29	+0.3
4,667,198	-		Pf1N1B4_4213	0.29	+0.2
4,667,198	-		Pf1N1B4_4213	0.29	+0.1
4,667,249	+		Pf1N1B4_4213	0.35	-1.7
4,667,249	+		Pf1N1B4_4213	0.35	-0.7
4,667,251	+		Pf1N1B4_4213	0.35	-0.8
4,667,252	-		Pf1N1B4_4213	0.36	-0.7
4,667,252	-		Pf1N1B4_4213	0.36	-1.3
4,667,252	-		Pf1N1B4_4213	0.36	-0.9
4,667,252	-		Pf1N1B4_4213	0.36	+0.0
4,667,252	-		Pf1N1B4_4213	0.36	+0.7
4,667,261	+		Pf1N1B4_4213	0.37	-0.1
4,667,433	+		Pf1N1B4_4213	0.56	-1.6
4,667,434	-		Pf1N1B4_4213	0.56	-0.8
4,667,436	-		Pf1N1B4_4213	0.57	-1.0
4,667,475	+		Pf1N1B4_4213	0.61	-0.2
4,667,499	-				-3.1
4,667,653	-		Pf1N1B4_4213	0.81	+0.2
4,667,653	-		Pf1N1B4_4213	0.81	-0.4
4,667,696	+		Pf1N1B4_4213	0.86	-1.0
4,667,696	+		Pf1N1B4_4213	0.86	-0.2
4,667,757	+				-2.8
4,667,757	+				+2.3
4,667,758	-				+0.6