Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4176 and Pf1N1B4_4177 are separated by 33 nucleotides Pf1N1B4_4177 and Pf1N1B4_4178 are separated by 167 nucleotides
Pf1N1B4_4176 - Alcohol dehydrogenase (EC 1.1.1.1), at 4,624,657 to 4,625,640
_4176
Pf1N1B4_4177 - transcriptional regulator, Crp/Fnr family, at 4,625,674 to 4,626,420
_4177
Pf1N1B4_4178 - Cell division protein FtsH (EC 3.4.24.-), at 4,626,588 to 4,628,375
_4178
Position (kb)
4625
4626
4627 Strain fitness (log2 ratio)
-2
-1
0
1 at 4624.764 kb on + strand, within Pf1N1B4_4176 at 4624.764 kb on + strand, within Pf1N1B4_4176 at 4625.036 kb on + strand at 4625.036 kb on + strand, within Pf1N1B4_4176 at 4625.036 kb on + strand, within Pf1N1B4_4176 at 4625.076 kb on - strand, within Pf1N1B4_4176 at 4625.076 kb on - strand, within Pf1N1B4_4176 at 4625.276 kb on + strand, within Pf1N1B4_4176 at 4625.555 kb on - strand at 4625.643 kb on + strand at 4625.644 kb on - strand at 4625.644 kb on - strand at 4625.644 kb on - strand at 4625.644 kb on - strand at 4625.825 kb on + strand, within Pf1N1B4_4177 at 4625.826 kb on - strand, within Pf1N1B4_4177 at 4626.056 kb on + strand, within Pf1N1B4_4177 at 4626.057 kb on - strand, within Pf1N1B4_4177 at 4626.389 kb on + strand at 4626.425 kb on + strand at 4626.453 kb on + strand at 4626.453 kb on + strand at 4626.695 kb on - strand at 4626.754 kb on + strand at 4626.794 kb on - strand, within Pf1N1B4_4178 at 4626.922 kb on + strand, within Pf1N1B4_4178 at 4626.923 kb on - strand, within Pf1N1B4_4178 at 4627.025 kb on + strand, within Pf1N1B4_4178 at 4627.027 kb on + strand, within Pf1N1B4_4178 at 4627.068 kb on - strand, within Pf1N1B4_4178 at 4627.068 kb on - strand, within Pf1N1B4_4178 at 4627.126 kb on + strand, within Pf1N1B4_4178 at 4627.198 kb on - strand, within Pf1N1B4_4178 at 4627.361 kb on + strand, within Pf1N1B4_4178 at 4627.362 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 4,624,764 + Pf1N1B4_4176 0.11 +0.3 4,624,764 + Pf1N1B4_4176 0.11 -0.1 4,625,036 + +1.3 4,625,036 + Pf1N1B4_4176 0.39 +0.0 4,625,036 + Pf1N1B4_4176 0.39 +0.1 4,625,076 - Pf1N1B4_4176 0.43 -0.7 4,625,076 - Pf1N1B4_4176 0.43 -1.3 4,625,276 + Pf1N1B4_4176 0.63 +0.0 4,625,555 - -0.6 4,625,643 + +1.6 4,625,644 - -0.7 4,625,644 - -1.3 4,625,644 - +0.6 4,625,644 - +0.3 4,625,825 + Pf1N1B4_4177 0.20 -1.5 4,625,826 - Pf1N1B4_4177 0.20 -0.6 4,626,056 + Pf1N1B4_4177 0.51 -0.1 4,626,057 - Pf1N1B4_4177 0.51 -1.0 4,626,389 + +0.3 4,626,425 + +0.1 4,626,453 + -0.9 4,626,453 + -1.0 4,626,695 - +0.3 4,626,754 + -0.5 4,626,794 - Pf1N1B4_4178 0.12 -0.5 4,626,922 + Pf1N1B4_4178 0.19 -0.8 4,626,923 - Pf1N1B4_4178 0.19 -0.4 4,627,025 + Pf1N1B4_4178 0.24 -0.4 4,627,027 + Pf1N1B4_4178 0.25 -1.8 4,627,068 - Pf1N1B4_4178 0.27 +0.0 4,627,068 - Pf1N1B4_4178 0.27 +0.3 4,627,126 + Pf1N1B4_4178 0.30 -0.3 4,627,198 - Pf1N1B4_4178 0.34 -0.4 4,627,361 + Pf1N1B4_4178 0.43 +0.5 4,627,362 - -0.7
Or see this region's nucleotide sequence