Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4015

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
4,464,929	+		Pf1N1B4_4013	0.72	-0.4
4,464,930	-		Pf1N1B4_4013	0.72	+0.6
4,464,930	-				+0.8
4,465,064	+		Pf1N1B4_4013	0.80	-1.1
4,465,251	+				+1.4
4,465,251	+				+1.0
4,465,252	-				+0.6
4,465,252	-				+0.2
4,465,351	+				+0.4
4,465,370	+				+2.0
4,465,370	+				+0.7
4,465,370	+				-0.1
4,465,409	+				-0.0
4,465,409	+				-0.2
4,465,410	-				-0.4
4,465,504	+				-0.1
4,465,505	-				+0.5
4,465,505	-				+0.1
4,465,521	+				-1.9
4,465,521	+				+1.1
4,465,522	-				-0.2
4,465,534	+				+0.9
4,465,535	-				-0.8
4,465,673	-		Pf1N1B4_4014	0.25	+0.7
4,465,673	-		Pf1N1B4_4014	0.25	-0.1
4,465,673	-		Pf1N1B4_4014	0.25	-0.5
4,465,673	-		Pf1N1B4_4014	0.25	-0.3
4,465,673	-		Pf1N1B4_4014	0.25	-0.2
4,465,728	+		Pf1N1B4_4014	0.42	+0.7
4,465,729	-		Pf1N1B4_4014	0.43	-0.2
4,465,729	-		Pf1N1B4_4014	0.43	+0.4
4,465,729	-		Pf1N1B4_4014	0.43	-0.5
4,465,729	-		Pf1N1B4_4014	0.43	-0.4
4,465,935	-		Pf1N1B4_4015	0.10	-0.6
4,465,935	-				-2.1
4,465,937	-		Pf1N1B4_4015	0.11	+0.1
4,465,938	+		Pf1N1B4_4015	0.11	-1.6
4,465,938	+		Pf1N1B4_4015	0.11	-0.5
4,465,938	+		Pf1N1B4_4015	0.11	-1.6
4,465,938	+		Pf1N1B4_4015	0.11	-1.7
4,465,938	+				-0.9
4,465,939	-		Pf1N1B4_4015	0.11	-1.1
4,465,939	-		Pf1N1B4_4015	0.11	+0.6
4,465,999	+		Pf1N1B4_4015	0.28	-0.7
4,466,001	+		Pf1N1B4_4015	0.29	-0.8
4,466,001	+		Pf1N1B4_4015	0.29	-1.2
4,466,002	-		Pf1N1B4_4015	0.29	-1.8
4,466,045	-		Pf1N1B4_4015	0.42	-0.5
4,466,120	+		Pf1N1B4_4015	0.63	-2.2
4,466,121	-		Pf1N1B4_4015	0.63	+0.4
4,466,197	+		Pf1N1B4_4015	0.85	-0.5
4,466,293	+				-0.5
4,466,294	-				+1.4
4,466,338	+				-0.8
4,466,339	-				+2.4
4,466,339	-				+2.1
4,466,363	+				-0.8
4,466,363	+				-0.4
4,466,364	-				-0.5
4,466,364	-				-1.5
4,466,487	+				-0.7
4,466,488	-				+0.2
4,466,979	-				-0.2
4,467,052	-				-0.9