Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3859

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
4,286,458	+		Pf1N1B4_3858	0.63	-0.9
4,286,459	-		Pf1N1B4_3858	0.63	-1.4
4,286,459	-		Pf1N1B4_3858	0.63	-1.2
4,286,482	+		Pf1N1B4_3858	0.65	-0.2
4,286,482	+		Pf1N1B4_3858	0.65	-2.6
4,286,518	+		Pf1N1B4_3858	0.68	-1.8
4,286,518	+		Pf1N1B4_3858	0.68	-1.9
4,286,518	+		Pf1N1B4_3858	0.68	-2.1
4,286,519	-		Pf1N1B4_3858	0.68	-0.9
4,286,887	+				+0.4
4,286,887	+				-1.0
4,286,888	-				-0.9
4,286,911	+				-0.3
4,286,911	+				-0.5
4,286,972	-				+0.8
4,287,314	-				-1.8
4,287,383	+				+0.7
4,287,449	+				-0.0
4,287,449	+				+0.1
4,287,450	-				+0.4
4,287,450	-				-0.7
4,287,450	-				+0.5
4,287,453	-				-0.4
4,287,453	-				-0.0
4,287,453	-				+1.8
4,287,453	-				-0.2
4,287,453	-				-0.4
4,287,519	+		Pf1N1B4_3859	0.23	-0.6
4,287,520	-		Pf1N1B4_3859	0.24	+0.4
4,287,520	-		Pf1N1B4_3859	0.24	+0.4
4,287,536	-		Pf1N1B4_3859	0.29	+1.3
4,287,536	-		Pf1N1B4_3859	0.29	+0.4
4,287,637	+		Pf1N1B4_3859	0.60	-0.3
4,287,637	+				-0.4
4,287,637	+				-0.2
4,287,638	-		Pf1N1B4_3859	0.60	-1.4
4,287,638	-		Pf1N1B4_3859	0.60	+0.6
4,287,638	-		Pf1N1B4_3859	0.60	-0.2
4,287,638	-		Pf1N1B4_3859	0.60	+0.4
4,287,638	-		Pf1N1B4_3859	0.60	-0.6
4,287,638	-		Pf1N1B4_3859	0.60	+0.6
4,287,638	-		Pf1N1B4_3859	0.60	+0.9
4,287,638	-		Pf1N1B4_3859	0.60	-1.9
4,287,708	-		Pf1N1B4_3859	0.82	-0.7
4,287,708	-		Pf1N1B4_3859	0.82	-0.2
4,287,813	+				-1.8
4,287,992	-				+0.6
4,288,012	+				+0.1
4,288,012	+				+2.0
4,288,012	+				-1.1
4,288,013	-				-0.4
4,288,013	-				-0.5
4,288,086	+				+0.2
4,288,086	+				+1.2
4,288,086	+				+0.4
4,288,174	+				+0.4
4,288,234	-				+1.0
4,288,237	+				-0.5
4,288,237	+				-1.7
4,288,237	+				-1.3
4,288,237	+				+0.2
4,288,237	+				+2.5
4,288,238	-				+1.4
4,288,238	-				-1.6
4,288,238	-				-0.4
4,288,375	-		Pf1N1B4_3860	0.12	+0.5
4,288,375	-		Pf1N1B4_3860	0.12	-1.1
4,288,395	+		Pf1N1B4_3860	0.14	-2.2
4,288,420	+		Pf1N1B4_3860	0.15	-1.3
4,288,595	+		Pf1N1B4_3860	0.25	-0.2
4,288,711	-		Pf1N1B4_3860	0.32	+0.4
4,288,711	-		Pf1N1B4_3860	0.32	-2.1