Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3819 and Pf1N1B4_3820 are separated by 184 nucleotides Pf1N1B4_3820 and Pf1N1B4_3821 are separated by 205 nucleotides
Pf1N1B4_3819 - Methylisocitrate lyase (EC 4.1.3.30), at 4,230,262 to 4,231,152
_3819
Pf1N1B4_3820 - 2-methylcitrate synthase (EC 2.3.3.5), at 4,231,337 to 4,232,464
_3820
Pf1N1B4_3821 - 2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79), at 4,232,670 to 4,235,264
_3821
Position (kb)
4231
4232
4233 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4230.394 kb on + strand, within Pf1N1B4_3819 at 4230.394 kb on + strand, within Pf1N1B4_3819 at 4230.395 kb on - strand, within Pf1N1B4_3819 at 4230.648 kb on + strand, within Pf1N1B4_3819 at 4230.651 kb on + strand at 4230.652 kb on - strand, within Pf1N1B4_3819 at 4230.759 kb on - strand, within Pf1N1B4_3819 at 4230.853 kb on + strand at 4230.853 kb on + strand, within Pf1N1B4_3819 at 4230.853 kb on + strand, within Pf1N1B4_3819 at 4230.853 kb on + strand at 4230.853 kb on + strand, within Pf1N1B4_3819 at 4230.854 kb on - strand, within Pf1N1B4_3819 at 4230.854 kb on - strand, within Pf1N1B4_3819 at 4230.854 kb on - strand, within Pf1N1B4_3819 at 4230.854 kb on - strand, within Pf1N1B4_3819 at 4230.854 kb on - strand, within Pf1N1B4_3819 at 4230.925 kb on + strand, within Pf1N1B4_3819 at 4230.925 kb on + strand, within Pf1N1B4_3819 at 4230.926 kb on - strand, within Pf1N1B4_3819 at 4230.926 kb on - strand, within Pf1N1B4_3819 at 4230.926 kb on - strand at 4231.019 kb on - strand, within Pf1N1B4_3819 at 4231.019 kb on - strand, within Pf1N1B4_3819 at 4231.151 kb on - strand at 4231.201 kb on - strand at 4231.201 kb on - strand at 4231.229 kb on + strand at 4231.229 kb on + strand at 4231.229 kb on + strand at 4231.353 kb on + strand at 4231.353 kb on + strand at 4231.436 kb on + strand at 4231.436 kb on + strand at 4231.437 kb on - strand at 4231.541 kb on + strand, within Pf1N1B4_3820 at 4231.542 kb on - strand, within Pf1N1B4_3820 at 4231.743 kb on - strand, within Pf1N1B4_3820 at 4231.955 kb on + strand, within Pf1N1B4_3820 at 4231.956 kb on - strand, within Pf1N1B4_3820 at 4231.956 kb on - strand, within Pf1N1B4_3820 at 4231.956 kb on - strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.127 kb on - strand at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.128 kb on + strand, within Pf1N1B4_3820 at 4232.129 kb on - strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.277 kb on - strand, within Pf1N1B4_3820 at 4232.277 kb on - strand, within Pf1N1B4_3820 at 4232.277 kb on - strand, within Pf1N1B4_3820 at 4232.292 kb on - strand, within Pf1N1B4_3820 at 4232.417 kb on + strand at 4232.417 kb on + strand at 4232.417 kb on + strand at 4232.418 kb on - strand at 4232.418 kb on - strand at 4232.418 kb on - strand at 4232.619 kb on + strand at 4232.620 kb on - strand at 4232.620 kb on - strand at 4232.646 kb on - strand at 4232.902 kb on + strand at 4232.903 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 4,230,394 + Pf1N1B4_3819 0.15 +0.6 4,230,394 + Pf1N1B4_3819 0.15 +0.6 4,230,395 - Pf1N1B4_3819 0.15 -0.8 4,230,648 + Pf1N1B4_3819 0.43 -0.4 4,230,651 + +0.8 4,230,652 - Pf1N1B4_3819 0.44 -0.2 4,230,759 - Pf1N1B4_3819 0.56 +2.1 4,230,853 + +1.4 4,230,853 + Pf1N1B4_3819 0.66 -0.7 4,230,853 + Pf1N1B4_3819 0.66 +0.4 4,230,853 + -1.6 4,230,853 + Pf1N1B4_3819 0.66 -1.0 4,230,854 - Pf1N1B4_3819 0.66 -1.4 4,230,854 - Pf1N1B4_3819 0.66 +0.4 4,230,854 - Pf1N1B4_3819 0.66 +0.4 4,230,854 - Pf1N1B4_3819 0.66 -0.1 4,230,854 - Pf1N1B4_3819 0.66 -0.1 4,230,925 + Pf1N1B4_3819 0.74 +0.4 4,230,925 + Pf1N1B4_3819 0.74 -1.1 4,230,926 - Pf1N1B4_3819 0.75 -0.9 4,230,926 - Pf1N1B4_3819 0.75 -0.1 4,230,926 - -0.4 4,231,019 - Pf1N1B4_3819 0.85 -1.0 4,231,019 - Pf1N1B4_3819 0.85 -1.2 4,231,151 - -0.6 4,231,201 - -1.4 4,231,201 - +1.0 4,231,229 + -1.2 4,231,229 + -1.5 4,231,229 + +0.0 4,231,353 + -2.5 4,231,353 + -0.2 4,231,436 + -1.0 4,231,436 + +1.4 4,231,437 - +1.4 4,231,541 + Pf1N1B4_3820 0.18 -1.2 4,231,542 - Pf1N1B4_3820 0.18 -3.1 4,231,743 - Pf1N1B4_3820 0.36 -2.4 4,231,955 + Pf1N1B4_3820 0.55 -0.8 4,231,956 - Pf1N1B4_3820 0.55 -0.7 4,231,956 - Pf1N1B4_3820 0.55 -0.4 4,231,956 - Pf1N1B4_3820 0.55 +1.0 4,232,126 + Pf1N1B4_3820 0.70 -0.9 4,232,126 + Pf1N1B4_3820 0.70 -2.1 4,232,126 + Pf1N1B4_3820 0.70 -1.1 4,232,126 + Pf1N1B4_3820 0.70 -2.0 4,232,126 + Pf1N1B4_3820 0.70 -0.6 4,232,126 + Pf1N1B4_3820 0.70 -0.4 4,232,126 + +1.0 4,232,126 + Pf1N1B4_3820 0.70 -1.2 4,232,126 + Pf1N1B4_3820 0.70 -1.9 4,232,127 - Pf1N1B4_3820 0.70 -2.0 4,232,127 - Pf1N1B4_3820 0.70 -1.8 4,232,127 - Pf1N1B4_3820 0.70 -0.1 4,232,127 - Pf1N1B4_3820 0.70 -0.9 4,232,127 - -0.4 4,232,127 - Pf1N1B4_3820 0.70 +0.2 4,232,128 + Pf1N1B4_3820 0.70 -2.5 4,232,129 - Pf1N1B4_3820 0.70 -1.2 4,232,276 + Pf1N1B4_3820 0.83 -3.0 4,232,276 + Pf1N1B4_3820 0.83 -1.2 4,232,276 + Pf1N1B4_3820 0.83 +1.0 4,232,276 + Pf1N1B4_3820 0.83 +0.7 4,232,276 + Pf1N1B4_3820 0.83 +0.7 4,232,276 + Pf1N1B4_3820 0.83 -1.5 4,232,276 + Pf1N1B4_3820 0.83 -1.4 4,232,276 + Pf1N1B4_3820 0.83 -1.0 4,232,277 - Pf1N1B4_3820 0.83 -1.0 4,232,277 - Pf1N1B4_3820 0.83 -1.0 4,232,277 - Pf1N1B4_3820 0.83 -0.0 4,232,292 - Pf1N1B4_3820 0.85 -1.4 4,232,417 + -1.0 4,232,417 + -0.9 4,232,417 + -1.0 4,232,418 - -1.3 4,232,418 - -1.0 4,232,418 - +0.1 4,232,619 + +0.1 4,232,620 - -1.0 4,232,620 - -0.2 4,232,646 - +0.4 4,232,902 + -1.0 4,232,903 - -2.1
Or see this region's nucleotide sequence