Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_377 and Pf1N1B4_378 are separated by 5 nucleotides Pf1N1B4_378 and Pf1N1B4_379 are separated by 153 nucleotides Pf1N1B4_379 and Pf1N1B4_380 are separated by 64 nucleotides Pf1N1B4_380 and Pf1N1B4_381 are separated by 293 nucleotides Pf1N1B4_381 and Pf1N1B4_382 are separated by 60 nucleotides
Pf1N1B4_377 - FIG00953242: hypothetical protein, at 448,805 to 449,032
_377
Pf1N1B4_378 - Carbon storage regulator, at 449,038 to 449,223
_378
Pf1N1B4_379 - Protein of unknown function UPF0060, at 449,377 to 449,709
_379
Pf1N1B4_380 - Oxidoreductase, short chain dehydrogenase/reductase family, at 449,774 to 450,589
_380
Pf1N1B4_381 - hypothetical protein, at 450,883 to 451,035
_381
Pf1N1B4_382 - Phosphoglycerol transferase and related proteins, alkaline phosphatase superfamily, at 451,096 to 453,189
_382
Position (kb) 449
450
451 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 448.804 kb on + strand at 448.804 kb on + strand at 449.082 kb on - strand, within Pf1N1B4_378 at 449.082 kb on - strand, within Pf1N1B4_378 at 449.082 kb on - strand, within Pf1N1B4_378 at 449.082 kb on - strand, within Pf1N1B4_378 at 449.098 kb on + strand, within Pf1N1B4_378 at 449.098 kb on + strand, within Pf1N1B4_378 at 449.098 kb on + strand, within Pf1N1B4_378 at 449.099 kb on - strand, within Pf1N1B4_378 at 449.099 kb on - strand, within Pf1N1B4_378 at 449.099 kb on - strand, within Pf1N1B4_378 at 449.099 kb on - strand, within Pf1N1B4_378 at 449.099 kb on - strand, within Pf1N1B4_378 at 449.216 kb on - strand at 449.312 kb on - strand at 449.312 kb on - strand at 449.358 kb on + strand at 449.359 kb on - strand at 449.510 kb on + strand, within Pf1N1B4_379 at 449.510 kb on + strand, within Pf1N1B4_379 at 449.510 kb on + strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.522 kb on + strand, within Pf1N1B4_379 at 449.523 kb on - strand, within Pf1N1B4_379 at 449.625 kb on + strand at 449.625 kb on + strand, within Pf1N1B4_379 at 449.626 kb on - strand, within Pf1N1B4_379 at 449.699 kb on + strand at 449.699 kb on + strand at 449.699 kb on + strand at 449.699 kb on + strand at 449.699 kb on + strand at 449.700 kb on - strand at 449.700 kb on - strand at 449.700 kb on - strand at 449.700 kb on - strand at 449.700 kb on - strand at 449.700 kb on - strand at 449.700 kb on - strand at 449.700 kb on - strand at 449.835 kb on + strand at 449.836 kb on - strand at 449.836 kb on - strand at 449.928 kb on + strand, within Pf1N1B4_380 at 449.934 kb on + strand, within Pf1N1B4_380 at 449.934 kb on + strand, within Pf1N1B4_380 at 449.934 kb on + strand, within Pf1N1B4_380 at 449.935 kb on - strand, within Pf1N1B4_380 at 449.935 kb on - strand, within Pf1N1B4_380 at 449.935 kb on - strand at 450.141 kb on + strand, within Pf1N1B4_380 at 450.141 kb on + strand, within Pf1N1B4_380 at 450.141 kb on + strand, within Pf1N1B4_380 at 450.141 kb on + strand, within Pf1N1B4_380 at 450.142 kb on - strand, within Pf1N1B4_380 at 450.142 kb on - strand, within Pf1N1B4_380 at 450.142 kb on - strand at 450.142 kb on - strand, within Pf1N1B4_380 at 450.142 kb on - strand, within Pf1N1B4_380 at 450.142 kb on - strand, within Pf1N1B4_380 at 450.641 kb on - strand at 450.727 kb on - strand at 450.735 kb on + strand at 450.752 kb on + strand at 450.753 kb on - strand at 450.757 kb on + strand at 450.803 kb on - strand at 450.810 kb on + strand at 450.810 kb on + strand at 450.816 kb on + strand at 450.817 kb on - strand at 450.819 kb on + strand at 450.819 kb on + strand at 450.819 kb on + strand at 450.819 kb on + strand at 450.819 kb on + strand at 450.819 kb on + strand at 450.820 kb on - strand at 450.820 kb on - strand at 450.820 kb on - strand at 450.846 kb on + strand at 450.897 kb on + strand at 450.910 kb on - strand at 450.923 kb on + strand, within Pf1N1B4_381 at 450.923 kb on + strand, within Pf1N1B4_381 at 450.923 kb on + strand, within Pf1N1B4_381 at 450.923 kb on + strand, within Pf1N1B4_381 at 450.923 kb on + strand, within Pf1N1B4_381 at 450.924 kb on - strand, within Pf1N1B4_381 at 451.004 kb on + strand, within Pf1N1B4_381 at 451.020 kb on - strand at 451.031 kb on + strand at 451.032 kb on - strand at 451.032 kb on - strand at 451.033 kb on + strand at 451.033 kb on + strand at 451.033 kb on + strand at 451.083 kb on - strand at 451.250 kb on + strand at 451.250 kb on + strand at 451.251 kb on - strand at 451.251 kb on - strand at 451.251 kb on - strand at 451.461 kb on + strand, within Pf1N1B4_382 at 451.462 kb on - strand, within Pf1N1B4_382
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 448,804 + -1.1 448,804 + +1.0 449,082 - Pf1N1B4_378 0.24 -0.4 449,082 - Pf1N1B4_378 0.24 +0.8 449,082 - Pf1N1B4_378 0.24 +0.1 449,082 - Pf1N1B4_378 0.24 -1.6 449,098 + Pf1N1B4_378 0.32 -0.0 449,098 + Pf1N1B4_378 0.32 -0.3 449,098 + Pf1N1B4_378 0.32 -0.6 449,099 - Pf1N1B4_378 0.33 +0.9 449,099 - Pf1N1B4_378 0.33 -1.2 449,099 - Pf1N1B4_378 0.33 +0.7 449,099 - Pf1N1B4_378 0.33 -0.8 449,099 - Pf1N1B4_378 0.33 +0.6 449,216 - +0.3 449,312 - -0.5 449,312 - -0.1 449,358 + -1.9 449,359 - +1.4 449,510 + Pf1N1B4_379 0.40 +0.1 449,510 + Pf1N1B4_379 0.40 -0.2 449,510 + Pf1N1B4_379 0.40 -1.2 449,511 - Pf1N1B4_379 0.40 +1.0 449,511 - Pf1N1B4_379 0.40 -0.4 449,511 - Pf1N1B4_379 0.40 -1.6 449,511 - Pf1N1B4_379 0.40 -1.6 449,511 - Pf1N1B4_379 0.40 +0.4 449,511 - Pf1N1B4_379 0.40 -0.6 449,522 + Pf1N1B4_379 0.44 -0.6 449,523 - Pf1N1B4_379 0.44 +0.2 449,625 + +1.4 449,625 + Pf1N1B4_379 0.74 -1.6 449,626 - Pf1N1B4_379 0.75 +0.5 449,699 + +0.7 449,699 + -0.9 449,699 + +0.4 449,699 + -0.0 449,699 + -0.4 449,700 - -0.7 449,700 - -1.0 449,700 - -1.1 449,700 - +0.1 449,700 - -0.4 449,700 - +0.4 449,700 - -0.2 449,700 - -0.6 449,835 + -0.6 449,836 - -1.0 449,836 - -0.2 449,928 + Pf1N1B4_380 0.19 +0.4 449,934 + Pf1N1B4_380 0.20 -0.7 449,934 + Pf1N1B4_380 0.20 -1.6 449,934 + Pf1N1B4_380 0.20 +0.8 449,935 - Pf1N1B4_380 0.20 -0.8 449,935 - Pf1N1B4_380 0.20 -1.5 449,935 - +0.4 450,141 + Pf1N1B4_380 0.45 +0.6 450,141 + Pf1N1B4_380 0.45 -1.5 450,141 + Pf1N1B4_380 0.45 -1.0 450,141 + Pf1N1B4_380 0.45 -1.5 450,142 - Pf1N1B4_380 0.45 -0.6 450,142 - Pf1N1B4_380 0.45 +2.7 450,142 - -1.0 450,142 - Pf1N1B4_380 0.45 +0.1 450,142 - Pf1N1B4_380 0.45 -1.6 450,142 - Pf1N1B4_380 0.45 -1.4 450,641 - +0.2 450,727 - -0.6 450,735 + -1.0 450,752 + +0.1 450,753 - -0.6 450,757 + -1.0 450,803 - -1.6 450,810 + -0.6 450,810 + +0.5 450,816 + -1.0 450,817 - -0.8 450,819 + +1.4 450,819 + -0.6 450,819 + -2.0 450,819 + -1.1 450,819 + -2.1 450,819 + +1.9 450,820 - -0.4 450,820 - -3.0 450,820 - -1.6 450,846 + +0.0 450,897 + -1.1 450,910 - +0.4 450,923 + Pf1N1B4_381 0.26 +0.0 450,923 + Pf1N1B4_381 0.26 +0.4 450,923 + Pf1N1B4_381 0.26 +0.9 450,923 + Pf1N1B4_381 0.26 +0.4 450,923 + Pf1N1B4_381 0.26 +0.6 450,924 - Pf1N1B4_381 0.27 -1.5 451,004 + Pf1N1B4_381 0.79 +0.4 451,020 - -1.5 451,031 + -0.6 451,032 - +0.5 451,032 - +0.7 451,033 + -1.0 451,033 + -0.6 451,033 + -0.5 451,083 - -0.4 451,250 + -1.3 451,250 + +1.5 451,251 - -1.1 451,251 - -0.2 451,251 - -0.4 451,461 + Pf1N1B4_382 0.17 +1.6 451,462 - Pf1N1B4_382 0.17 -1.0
Or see this region's nucleotide sequence