Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3785 and Pf1N1B4_3786 are separated by 153 nucleotides Pf1N1B4_3786 and Pf1N1B4_3787 are separated by 83 nucleotides Pf1N1B4_3787 and Pf1N1B4_3788 are separated by 306 nucleotides Pf1N1B4_3788 and Pf1N1B4_3789 are separated by 215 nucleotides
Pf1N1B4_3785 - FOG: Ankyrin repeat, at 4,194,975 to 4,195,493
_3785
Pf1N1B4_3786 - FIG00955145: hypothetical protein, at 4,195,647 to 4,196,177
_3786
Pf1N1B4_3787 - FIG00960829: hypothetical protein, at 4,196,261 to 4,196,452
_3787
Pf1N1B4_3788 - FIG00957412: hypothetical protein, at 4,196,759 to 4,196,992
_3788
Pf1N1B4_3789 - Superfamily II DNA/RNA helicases, SNF2 family, at 4,197,208 to 4,197,549
_3789
Position (kb)
4196
4197 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4195.590 kb on - strand at 4195.590 kb on - strand at 4195.590 kb on - strand at 4195.590 kb on - strand at 4195.590 kb on - strand at 4195.595 kb on + strand at 4195.595 kb on + strand at 4195.595 kb on + strand at 4195.595 kb on + strand at 4195.595 kb on + strand at 4195.595 kb on + strand at 4195.596 kb on - strand at 4195.596 kb on - strand at 4195.596 kb on - strand at 4195.596 kb on - strand at 4195.596 kb on - strand at 4195.596 kb on - strand at 4195.596 kb on - strand at 4195.596 kb on - strand at 4195.788 kb on + strand, within Pf1N1B4_3786 at 4195.788 kb on + strand, within Pf1N1B4_3786 at 4195.788 kb on + strand, within Pf1N1B4_3786 at 4195.789 kb on - strand, within Pf1N1B4_3786 at 4195.872 kb on + strand at 4195.872 kb on + strand, within Pf1N1B4_3786 at 4195.872 kb on + strand, within Pf1N1B4_3786 at 4195.872 kb on + strand, within Pf1N1B4_3786 at 4195.873 kb on - strand, within Pf1N1B4_3786 at 4195.873 kb on - strand, within Pf1N1B4_3786 at 4195.873 kb on - strand, within Pf1N1B4_3786 at 4195.873 kb on - strand, within Pf1N1B4_3786 at 4195.873 kb on - strand, within Pf1N1B4_3786 at 4195.873 kb on - strand, within Pf1N1B4_3786 at 4195.873 kb on - strand, within Pf1N1B4_3786 at 4196.022 kb on + strand, within Pf1N1B4_3786 at 4196.185 kb on + strand at 4196.193 kb on + strand at 4196.194 kb on - strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.229 kb on - strand at 4196.238 kb on + strand at 4196.335 kb on + strand, within Pf1N1B4_3787 at 4196.335 kb on + strand, within Pf1N1B4_3787 at 4196.336 kb on - strand, within Pf1N1B4_3787 at 4196.336 kb on - strand, within Pf1N1B4_3787 at 4196.336 kb on - strand, within Pf1N1B4_3787 at 4196.336 kb on - strand, within Pf1N1B4_3787 at 4196.377 kb on - strand, within Pf1N1B4_3787 at 4196.377 kb on - strand, within Pf1N1B4_3787 at 4196.571 kb on + strand at 4196.571 kb on + strand at 4196.572 kb on - strand at 4196.705 kb on + strand at 4196.718 kb on + strand at 4196.718 kb on + strand at 4196.718 kb on + strand at 4196.719 kb on - strand at 4196.719 kb on - strand at 4196.719 kb on - strand at 4196.719 kb on - strand at 4196.724 kb on + strand at 4196.724 kb on + strand at 4196.724 kb on + strand at 4196.725 kb on - strand at 4196.725 kb on - strand at 4196.725 kb on - strand at 4196.725 kb on - strand at 4196.725 kb on - strand at 4196.977 kb on + strand at 4196.977 kb on + strand at 4196.978 kb on - strand at 4196.978 kb on - strand at 4196.994 kb on - strand at 4196.994 kb on - strand at 4197.036 kb on + strand at 4197.037 kb on - strand at 4197.037 kb on - strand at 4197.099 kb on + strand at 4197.099 kb on + strand at 4197.100 kb on - strand at 4197.100 kb on - strand at 4197.100 kb on - strand at 4197.100 kb on - strand at 4197.100 kb on - strand at 4197.100 kb on - strand at 4197.182 kb on - strand at 4197.182 kb on - strand at 4197.304 kb on + strand, within Pf1N1B4_3789 at 4197.304 kb on + strand, within Pf1N1B4_3789 at 4197.305 kb on - strand, within Pf1N1B4_3789 at 4197.430 kb on + strand, within Pf1N1B4_3789 at 4197.430 kb on + strand, within Pf1N1B4_3789 at 4197.431 kb on - strand, within Pf1N1B4_3789 at 4197.439 kb on + strand, within Pf1N1B4_3789 at 4197.439 kb on + strand, within Pf1N1B4_3789
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 4,195,590 - -1.8 4,195,590 - +0.1 4,195,590 - -1.8 4,195,590 - +1.7 4,195,590 - -0.3 4,195,595 + -0.8 4,195,595 + +0.3 4,195,595 + +0.3 4,195,595 + +0.4 4,195,595 + +0.8 4,195,595 + +0.1 4,195,596 - -0.3 4,195,596 - +0.2 4,195,596 - +0.5 4,195,596 - -0.9 4,195,596 - -2.8 4,195,596 - +0.3 4,195,596 - +2.4 4,195,596 - +0.5 4,195,788 + Pf1N1B4_3786 0.27 -1.2 4,195,788 + Pf1N1B4_3786 0.27 +1.4 4,195,788 + Pf1N1B4_3786 0.27 -0.8 4,195,789 - Pf1N1B4_3786 0.27 -0.2 4,195,872 + -1.2 4,195,872 + Pf1N1B4_3786 0.42 +1.6 4,195,872 + Pf1N1B4_3786 0.42 +0.2 4,195,872 + Pf1N1B4_3786 0.42 +0.6 4,195,873 - Pf1N1B4_3786 0.43 +0.8 4,195,873 - Pf1N1B4_3786 0.43 -0.0 4,195,873 - Pf1N1B4_3786 0.43 -0.4 4,195,873 - Pf1N1B4_3786 0.43 +0.1 4,195,873 - Pf1N1B4_3786 0.43 +1.2 4,195,873 - Pf1N1B4_3786 0.43 +0.2 4,195,873 - Pf1N1B4_3786 0.43 +0.3 4,196,022 + Pf1N1B4_3786 0.71 -0.5 4,196,185 + -1.9 4,196,193 + +0.2 4,196,194 - -1.2 4,196,228 + -1.1 4,196,228 + +1.1 4,196,228 + -0.1 4,196,228 + -0.7 4,196,228 + -0.9 4,196,228 + +3.0 4,196,228 + +0.1 4,196,229 - -2.0 4,196,238 + +1.1 4,196,335 + Pf1N1B4_3787 0.39 +0.6 4,196,335 + Pf1N1B4_3787 0.39 -1.5 4,196,336 - Pf1N1B4_3787 0.39 +1.0 4,196,336 - Pf1N1B4_3787 0.39 -0.3 4,196,336 - Pf1N1B4_3787 0.39 -1.3 4,196,336 - Pf1N1B4_3787 0.39 -0.7 4,196,377 - Pf1N1B4_3787 0.60 -0.4 4,196,377 - Pf1N1B4_3787 0.60 -2.3 4,196,571 + -0.3 4,196,571 + -0.2 4,196,572 - -0.5 4,196,705 + +0.2 4,196,718 + -1.1 4,196,718 + -0.1 4,196,718 + -0.8 4,196,719 - -1.1 4,196,719 - +0.4 4,196,719 - +1.0 4,196,719 - -0.9 4,196,724 + +2.0 4,196,724 + +0.2 4,196,724 + +0.5 4,196,725 - -0.1 4,196,725 - -1.1 4,196,725 - +1.1 4,196,725 - -1.7 4,196,725 - +0.4 4,196,977 + -0.4 4,196,977 + +0.4 4,196,978 - -1.4 4,196,978 - +0.6 4,196,994 - +1.4 4,196,994 - +0.1 4,197,036 + +0.1 4,197,037 - -1.9 4,197,037 - -0.2 4,197,099 + -0.9 4,197,099 + +1.4 4,197,100 - +1.4 4,197,100 - +0.2 4,197,100 - +0.4 4,197,100 - -0.4 4,197,100 - +0.5 4,197,100 - -0.2 4,197,182 - +1.4 4,197,182 - +1.4 4,197,304 + Pf1N1B4_3789 0.28 -1.3 4,197,304 + Pf1N1B4_3789 0.28 -0.6 4,197,305 - Pf1N1B4_3789 0.28 -1.4 4,197,430 + Pf1N1B4_3789 0.65 -0.0 4,197,430 + Pf1N1B4_3789 0.65 -0.4 4,197,431 - Pf1N1B4_3789 0.65 -1.1 4,197,439 + Pf1N1B4_3789 0.68 -1.2 4,197,439 + Pf1N1B4_3789 0.68 -0.5
Or see this region's nucleotide sequence