Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3715

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
4,122,433	+		Pf1N1B4_3713	0.65	-0.2
4,122,544	+		Pf1N1B4_3713	0.75	+0.1
4,122,545	-				-0.5
4,123,126	+		Pf1N1B4_3714	0.56	+0.3
4,123,126	+		Pf1N1B4_3714	0.56	-1.3
4,123,211	-		Pf1N1B4_3714	0.71	-0.2
4,123,736	-				+1.0
4,123,736	-				+1.4
4,123,848	+		Pf1N1B4_3715	0.64	-0.6
4,123,848	+		Pf1N1B4_3715	0.64	-0.2
4,123,848	+		Pf1N1B4_3715	0.64	-1.8
4,123,848	+		Pf1N1B4_3715	0.64	+0.2
4,123,848	+		Pf1N1B4_3715	0.64	-1.4
4,123,848	+				-0.2
4,123,849	-		Pf1N1B4_3715	0.64	-2.0
4,123,849	-		Pf1N1B4_3715	0.64	+2.0
4,123,849	-		Pf1N1B4_3715	0.64	-1.5
4,123,849	-		Pf1N1B4_3715	0.64	+1.8
4,123,967	-		Pf1N1B4_3715	0.80	-1.8
4,124,049	-				-1.0
4,124,214	+				+0.7
4,124,214	+				+0.9
4,124,214	+				-0.6
4,124,214	+				-0.3
4,124,214	+				-0.8
4,124,214	+				-1.1
4,124,214	+				-1.0
4,124,214	+				+0.0
4,124,215	-				-2.2
4,124,215	-				-1.8
4,124,215	-				-1.0
4,124,215	-				-0.1
4,124,215	-				-0.2
4,124,215	-				+1.6
4,124,215	-				-1.4
4,124,215	-				-1.6
4,124,215	-				-1.4
4,124,215	-				-1.0
4,124,215	-				+0.1
4,124,219	-				+0.3
4,124,219	-				-0.8
4,124,219	-				-1.1
4,124,251	-				+0.1
4,124,251	-				+0.0
4,124,252	+				+0.8
4,124,254	+				+0.0
4,124,254	+				-0.1
4,124,254	+				+1.4
4,124,254	+				-0.2
4,124,255	-				-1.0
4,124,255	-				-0.8
4,124,255	-				-0.7
4,124,263	+				-1.6
4,124,290	+				-0.6
4,124,328	-		Pf1N1B4_3716	0.12	+0.4
4,124,697	+				-0.6
4,124,812	+				-0.8
4,124,813	-				-0.2
4,124,973	+		Pf1N1B4_3717	0.21	-1.6