Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3618 and Pf1N1B4_3619 overlap by 4 nucleotides Pf1N1B4_3619 and Pf1N1B4_3620 are separated by 3 nucleotides Pf1N1B4_3620 and Pf1N1B4_3621 are separated by 3 nucleotides
Pf1N1B4_3618 - Flagellar biosynthesis protein FliP, at 4,026,663 to 4,027,421
_3618
Pf1N1B4_3619 - Flagellar biosynthesis protein FliQ, at 4,027,418 to 4,027,687
_3619
Pf1N1B4_3620 - Flagellar biosynthesis protein FliR, at 4,027,691 to 4,028,473
_3620
Pf1N1B4_3621 - Flagellar biosynthesis protein FlhB, at 4,028,477 to 4,029,616
_3621
Position (kb) 4027
4028
4029 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4027.437 kb on + strand at 4027.643 kb on + strand, within Pf1N1B4_3619 at 4027.643 kb on + strand, within Pf1N1B4_3619 at 4027.643 kb on + strand, within Pf1N1B4_3619 at 4027.643 kb on + strand, within Pf1N1B4_3619 at 4027.643 kb on + strand, within Pf1N1B4_3619 at 4027.643 kb on + strand, within Pf1N1B4_3619 at 4027.643 kb on + strand, within Pf1N1B4_3619 at 4027.643 kb on + strand, within Pf1N1B4_3619 at 4027.643 kb on + strand, within Pf1N1B4_3619 at 4027.644 kb on - strand, within Pf1N1B4_3619 at 4027.644 kb on - strand at 4027.644 kb on - strand, within Pf1N1B4_3619 at 4027.646 kb on - strand, within Pf1N1B4_3619 at 4027.646 kb on - strand, within Pf1N1B4_3619 at 4027.658 kb on + strand, within Pf1N1B4_3619 at 4027.666 kb on + strand at 4027.666 kb on + strand at 4027.666 kb on + strand at 4027.829 kb on + strand, within Pf1N1B4_3620 at 4027.830 kb on - strand, within Pf1N1B4_3620 at 4028.233 kb on - strand at 4028.448 kb on + strand at 4028.449 kb on - strand at 4028.581 kb on + strand at 4028.581 kb on + strand at 4028.581 kb on + strand at 4028.582 kb on - strand at 4028.582 kb on - strand at 4028.582 kb on - strand at 4029.058 kb on + strand, within Pf1N1B4_3621 at 4029.058 kb on + strand, within Pf1N1B4_3621 at 4029.059 kb on - strand, within Pf1N1B4_3621 at 4029.059 kb on - strand, within Pf1N1B4_3621 at 4029.059 kb on - strand, within Pf1N1B4_3621 at 4029.187 kb on + strand, within Pf1N1B4_3621 at 4029.187 kb on + strand, within Pf1N1B4_3621 at 4029.187 kb on + strand, within Pf1N1B4_3621 at 4029.297 kb on - strand, within Pf1N1B4_3621 at 4029.314 kb on + strand, within Pf1N1B4_3621 at 4029.315 kb on - strand, within Pf1N1B4_3621 at 4029.315 kb on - strand at 4029.404 kb on - strand at 4029.446 kb on + strand, within Pf1N1B4_3621 at 4029.446 kb on + strand, within Pf1N1B4_3621 at 4029.446 kb on + strand, within Pf1N1B4_3621 at 4029.446 kb on + strand, within Pf1N1B4_3621 at 4029.446 kb on + strand, within Pf1N1B4_3621 at 4029.449 kb on + strand, within Pf1N1B4_3621 at 4029.449 kb on + strand, within Pf1N1B4_3621
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 4,027,437 + +1.9 4,027,643 + Pf1N1B4_3619 0.83 -1.0 4,027,643 + Pf1N1B4_3619 0.83 +0.6 4,027,643 + Pf1N1B4_3619 0.83 -0.6 4,027,643 + Pf1N1B4_3619 0.83 -0.4 4,027,643 + Pf1N1B4_3619 0.83 +0.2 4,027,643 + Pf1N1B4_3619 0.83 +0.9 4,027,643 + Pf1N1B4_3619 0.83 -0.4 4,027,643 + Pf1N1B4_3619 0.83 -0.8 4,027,643 + Pf1N1B4_3619 0.83 -1.8 4,027,644 - Pf1N1B4_3619 0.84 -3.3 4,027,644 - -1.5 4,027,644 - Pf1N1B4_3619 0.84 +0.1 4,027,646 - Pf1N1B4_3619 0.84 +0.4 4,027,646 - Pf1N1B4_3619 0.84 -0.3 4,027,658 + Pf1N1B4_3619 0.89 -0.3 4,027,666 + -0.2 4,027,666 + +0.8 4,027,666 + -1.2 4,027,829 + Pf1N1B4_3620 0.18 -0.8 4,027,830 - Pf1N1B4_3620 0.18 -0.8 4,028,233 - -0.2 4,028,448 + -1.5 4,028,449 - -0.5 4,028,581 + -1.2 4,028,581 + +0.6 4,028,581 + +1.1 4,028,582 - +0.2 4,028,582 - +0.6 4,028,582 - -0.4 4,029,058 + Pf1N1B4_3621 0.51 +0.5 4,029,058 + Pf1N1B4_3621 0.51 +0.8 4,029,059 - Pf1N1B4_3621 0.51 -1.5 4,029,059 - Pf1N1B4_3621 0.51 -0.5 4,029,059 - Pf1N1B4_3621 0.51 -2.1 4,029,187 + Pf1N1B4_3621 0.62 +1.0 4,029,187 + Pf1N1B4_3621 0.62 +1.4 4,029,187 + Pf1N1B4_3621 0.62 +0.8 4,029,297 - Pf1N1B4_3621 0.72 +0.6 4,029,314 + Pf1N1B4_3621 0.73 +0.1 4,029,315 - Pf1N1B4_3621 0.74 -1.0 4,029,315 - +1.4 4,029,404 - +1.4 4,029,446 + Pf1N1B4_3621 0.85 +0.2 4,029,446 + Pf1N1B4_3621 0.85 +1.2 4,029,446 + Pf1N1B4_3621 0.85 -1.0 4,029,446 + Pf1N1B4_3621 0.85 -0.2 4,029,446 + Pf1N1B4_3621 0.85 +1.0 4,029,449 + Pf1N1B4_3621 0.85 +0.4 4,029,449 + Pf1N1B4_3621 0.85 -0.1
Or see this region's nucleotide sequence