Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3319 and Pf1N1B4_3320 are separated by 147 nucleotides Pf1N1B4_3320 and Pf1N1B4_3321 are separated by 1 nucleotides Pf1N1B4_3321 and Pf1N1B4_3322 overlap by 32 nucleotides Pf1N1B4_3322 and Pf1N1B4_3323 are separated by 557 nucleotides
Pf1N1B4_3319 - Folate-dependent protein for Fe/S cluster synthesis/repair in oxidative stress, at 3,707,593 to 3,708,534
_3319
Pf1N1B4_3320 - YgfY COG2938, at 3,708,682 to 3,708,936
_3320
Pf1N1B4_3321 - FIG00955375: hypothetical protein, at 3,708,938 to 3,709,372
_3321
Pf1N1B4_3322 - L-aspartate oxidase (EC 1.4.3.16), at 3,709,341 to 3,710,957
_3322
Pf1N1B4_3323 - RNA polymerase sigma-H factor AlgT, at 3,711,515 to 3,712,096
_3323
Position (kb)
3709
3710
3711 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3708.570 kb on + strand at 3708.570 kb on + strand at 3708.570 kb on + strand at 3708.571 kb on - strand at 3708.593 kb on + strand at 3708.652 kb on + strand at 3708.652 kb on + strand at 3708.652 kb on + strand at 3708.652 kb on + strand at 3708.652 kb on + strand at 3708.652 kb on + strand at 3708.653 kb on - strand at 3708.653 kb on - strand at 3708.653 kb on - strand at 3708.654 kb on + strand at 3708.654 kb on + strand at 3708.654 kb on + strand at 3708.654 kb on + strand at 3708.654 kb on + strand at 3708.654 kb on + strand at 3708.654 kb on + strand at 3708.654 kb on + strand at 3708.655 kb on - strand at 3708.655 kb on - strand at 3708.655 kb on - strand at 3708.655 kb on - strand at 3708.655 kb on - strand at 3708.655 kb on - strand at 3708.655 kb on - strand at 3708.655 kb on - strand at 3708.655 kb on - strand at 3708.655 kb on - strand at 3708.655 kb on - strand at 3708.655 kb on - strand at 3708.665 kb on + strand at 3708.666 kb on - strand at 3708.811 kb on + strand, within Pf1N1B4_3320 at 3708.934 kb on + strand at 3708.934 kb on + strand at 3708.934 kb on + strand at 3708.934 kb on + strand at 3708.983 kb on + strand, within Pf1N1B4_3321 at 3709.236 kb on + strand at 3709.237 kb on - strand, within Pf1N1B4_3321 at 3709.370 kb on + strand at 3709.370 kb on + strand at 3709.370 kb on + strand at 3709.371 kb on - strand at 3709.371 kb on - strand at 3709.371 kb on - strand at 3709.371 kb on - strand at 3709.371 kb on - strand at 3709.516 kb on + strand, within Pf1N1B4_3322 at 3709.516 kb on + strand, within Pf1N1B4_3322 at 3709.516 kb on + strand, within Pf1N1B4_3322 at 3709.516 kb on + strand, within Pf1N1B4_3322 at 3709.516 kb on + strand, within Pf1N1B4_3322 at 3709.517 kb on - strand, within Pf1N1B4_3322 at 3709.517 kb on - strand, within Pf1N1B4_3322 at 3709.517 kb on - strand, within Pf1N1B4_3322 at 3709.521 kb on - strand, within Pf1N1B4_3322 at 3709.521 kb on - strand, within Pf1N1B4_3322 at 3709.521 kb on - strand, within Pf1N1B4_3322 at 3709.525 kb on + strand, within Pf1N1B4_3322 at 3709.525 kb on + strand, within Pf1N1B4_3322 at 3709.525 kb on + strand, within Pf1N1B4_3322 at 3709.526 kb on - strand, within Pf1N1B4_3322 at 3709.609 kb on + strand, within Pf1N1B4_3322 at 3709.610 kb on - strand at 3709.846 kb on + strand, within Pf1N1B4_3322 at 3709.903 kb on + strand, within Pf1N1B4_3322 at 3709.903 kb on + strand at 3709.904 kb on - strand, within Pf1N1B4_3322 at 3709.904 kb on - strand, within Pf1N1B4_3322 at 3709.904 kb on - strand, within Pf1N1B4_3322 at 3709.904 kb on - strand, within Pf1N1B4_3322 at 3709.945 kb on + strand, within Pf1N1B4_3322 at 3709.972 kb on + strand, within Pf1N1B4_3322 at 3709.972 kb on + strand, within Pf1N1B4_3322 at 3709.973 kb on - strand, within Pf1N1B4_3322 at 3710.029 kb on + strand, within Pf1N1B4_3322 at 3710.318 kb on + strand, within Pf1N1B4_3322 at 3710.318 kb on + strand, within Pf1N1B4_3322 at 3710.320 kb on + strand, within Pf1N1B4_3322 at 3710.320 kb on + strand, within Pf1N1B4_3322 at 3710.320 kb on + strand, within Pf1N1B4_3322 at 3710.320 kb on + strand, within Pf1N1B4_3322 at 3710.321 kb on - strand, within Pf1N1B4_3322 at 3710.321 kb on - strand, within Pf1N1B4_3322 at 3710.321 kb on - strand, within Pf1N1B4_3322 at 3710.321 kb on - strand, within Pf1N1B4_3322 at 3710.326 kb on + strand, within Pf1N1B4_3322 at 3710.327 kb on - strand, within Pf1N1B4_3322 at 3710.327 kb on - strand, within Pf1N1B4_3322 at 3710.379 kb on - strand, within Pf1N1B4_3322 at 3710.410 kb on + strand, within Pf1N1B4_3322 at 3710.410 kb on + strand, within Pf1N1B4_3322 at 3710.410 kb on + strand, within Pf1N1B4_3322 at 3710.410 kb on + strand, within Pf1N1B4_3322 at 3710.410 kb on + strand, within Pf1N1B4_3322 at 3710.410 kb on + strand, within Pf1N1B4_3322 at 3710.411 kb on - strand, within Pf1N1B4_3322 at 3711.041 kb on + strand at 3711.041 kb on + strand at 3711.044 kb on + strand at 3711.044 kb on + strand at 3711.044 kb on + strand at 3711.044 kb on + strand at 3711.044 kb on + strand at 3711.044 kb on + strand at 3711.044 kb on + strand at 3711.044 kb on + strand at 3711.044 kb on + strand at 3711.044 kb on + strand at 3711.044 kb on + strand at 3711.044 kb on + strand at 3711.044 kb on + strand at 3711.044 kb on + strand at 3711.044 kb on + strand at 3711.046 kb on + strand at 3711.046 kb on + strand at 3711.046 kb on + strand at 3711.046 kb on + strand at 3711.046 kb on + strand at 3711.046 kb on + strand at 3711.046 kb on + strand at 3711.048 kb on + strand at 3711.048 kb on + strand at 3711.048 kb on + strand at 3711.048 kb on + strand at 3711.048 kb on + strand at 3711.097 kb on + strand at 3711.097 kb on + strand at 3711.165 kb on + strand at 3711.165 kb on + strand at 3711.165 kb on + strand at 3711.176 kb on + strand at 3711.176 kb on + strand at 3711.224 kb on + strand at 3711.224 kb on + strand at 3711.226 kb on + strand at 3711.226 kb on + strand at 3711.226 kb on + strand at 3711.230 kb on + strand at 3711.347 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 3,708,570 + -0.3 3,708,570 + -0.5 3,708,570 + -2.0 3,708,571 - +0.0 3,708,593 + -0.5 3,708,652 + +0.3 3,708,652 + -0.0 3,708,652 + -0.7 3,708,652 + +0.7 3,708,652 + -1.9 3,708,652 + -0.2 3,708,653 - -0.3 3,708,653 - -1.1 3,708,653 - -0.7 3,708,654 + +0.8 3,708,654 + -0.5 3,708,654 + -0.4 3,708,654 + -0.4 3,708,654 + -2.0 3,708,654 + -1.5 3,708,654 + -0.1 3,708,654 + +1.3 3,708,655 - +0.3 3,708,655 - +1.3 3,708,655 - +0.9 3,708,655 - +1.3 3,708,655 - -0.7 3,708,655 - +2.3 3,708,655 - +1.3 3,708,655 - -0.3 3,708,655 - -2.0 3,708,655 - +0.7 3,708,655 - -1.0 3,708,655 - +2.3 3,708,665 + -1.0 3,708,666 - +0.3 3,708,811 + Pf1N1B4_3320 0.51 +1.3 3,708,934 + +0.7 3,708,934 + -1.1 3,708,934 + +0.1 3,708,934 + -0.3 3,708,983 + Pf1N1B4_3321 0.10 -1.9 3,709,236 + +2.2 3,709,237 - Pf1N1B4_3321 0.69 -0.4 3,709,370 + -0.1 3,709,370 + -1.3 3,709,370 + -0.2 3,709,371 - -2.5 3,709,371 - -0.4 3,709,371 - -1.5 3,709,371 - -0.4 3,709,371 - -1.8 3,709,516 + Pf1N1B4_3322 0.11 +0.4 3,709,516 + Pf1N1B4_3322 0.11 +0.1 3,709,516 + Pf1N1B4_3322 0.11 +1.3 3,709,516 + Pf1N1B4_3322 0.11 +0.1 3,709,516 + Pf1N1B4_3322 0.11 -2.1 3,709,517 - Pf1N1B4_3322 0.11 -1.5 3,709,517 - Pf1N1B4_3322 0.11 +0.6 3,709,517 - Pf1N1B4_3322 0.11 -0.0 3,709,521 - Pf1N1B4_3322 0.11 -1.1 3,709,521 - Pf1N1B4_3322 0.11 -1.3 3,709,521 - Pf1N1B4_3322 0.11 -0.3 3,709,525 + Pf1N1B4_3322 0.11 -0.5 3,709,525 + Pf1N1B4_3322 0.11 -1.5 3,709,525 + Pf1N1B4_3322 0.11 -1.2 3,709,526 - Pf1N1B4_3322 0.11 +1.3 3,709,609 + Pf1N1B4_3322 0.17 -1.1 3,709,610 - -1.3 3,709,846 + Pf1N1B4_3322 0.31 -1.3 3,709,903 + Pf1N1B4_3322 0.35 -1.0 3,709,903 + +0.3 3,709,904 - Pf1N1B4_3322 0.35 +0.4 3,709,904 - Pf1N1B4_3322 0.35 +0.3 3,709,904 - Pf1N1B4_3322 0.35 -0.0 3,709,904 - Pf1N1B4_3322 0.35 -1.3 3,709,945 + Pf1N1B4_3322 0.37 -0.3 3,709,972 + Pf1N1B4_3322 0.39 -0.6 3,709,972 + Pf1N1B4_3322 0.39 -0.2 3,709,973 - Pf1N1B4_3322 0.39 -1.3 3,710,029 + Pf1N1B4_3322 0.43 -1.5 3,710,318 + Pf1N1B4_3322 0.60 -0.6 3,710,318 + Pf1N1B4_3322 0.60 -0.3 3,710,320 + Pf1N1B4_3322 0.61 -0.7 3,710,320 + Pf1N1B4_3322 0.61 -0.7 3,710,320 + Pf1N1B4_3322 0.61 -2.6 3,710,320 + Pf1N1B4_3322 0.61 -1.0 3,710,321 - Pf1N1B4_3322 0.61 -1.0 3,710,321 - Pf1N1B4_3322 0.61 -1.4 3,710,321 - Pf1N1B4_3322 0.61 -1.1 3,710,321 - Pf1N1B4_3322 0.61 -0.6 3,710,326 + Pf1N1B4_3322 0.61 -1.5 3,710,327 - Pf1N1B4_3322 0.61 -0.4 3,710,327 - Pf1N1B4_3322 0.61 +0.6 3,710,379 - Pf1N1B4_3322 0.64 -0.3 3,710,410 + Pf1N1B4_3322 0.66 +0.3 3,710,410 + Pf1N1B4_3322 0.66 -0.0 3,710,410 + Pf1N1B4_3322 0.66 -1.6 3,710,410 + Pf1N1B4_3322 0.66 -1.3 3,710,410 + Pf1N1B4_3322 0.66 -0.0 3,710,410 + Pf1N1B4_3322 0.66 -2.1 3,710,411 - Pf1N1B4_3322 0.66 -1.3 3,711,041 + +1.3 3,711,041 + +0.6 3,711,044 + -0.0 3,711,044 + -0.4 3,711,044 + +1.3 3,711,044 + -0.0 3,711,044 + -0.7 3,711,044 + +2.9 3,711,044 + +2.1 3,711,044 + +0.3 3,711,044 + -0.3 3,711,044 + +0.9 3,711,044 + +0.3 3,711,044 + -0.7 3,711,044 + -0.3 3,711,044 + +1.1 3,711,044 + -0.5 3,711,046 + +1.3 3,711,046 + +1.7 3,711,046 + +1.1 3,711,046 + -0.1 3,711,046 + +1.3 3,711,046 + -0.0 3,711,046 + -1.0 3,711,048 + +1.9 3,711,048 + -1.7 3,711,048 + -0.6 3,711,048 + +1.9 3,711,048 + -1.0 3,711,097 + +0.1 3,711,097 + -0.7 3,711,165 + +2.1 3,711,165 + +0.9 3,711,165 + +0.3 3,711,176 + -0.1 3,711,176 + -2.9 3,711,224 + -0.9 3,711,224 + +0.3 3,711,226 + -0.7 3,711,226 + -0.4 3,711,226 + -0.1 3,711,230 + +0.7 3,711,347 + -0.4
Or see this region's nucleotide sequence