Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_318 and Pf1N1B4_319 are separated by 15 nucleotides Pf1N1B4_319 and Pf1N1B4_320 are separated by 47 nucleotides Pf1N1B4_320 and Pf1N1B4_321 are separated by 32 nucleotides
Pf1N1B4_318 - NOHBY730; no homolog in Saccharomyces cerevisiae, at 374,762 to 375,613
_318
Pf1N1B4_319 - Transcriptional regulator, GntR family, at 375,629 to 376,306
_319
Pf1N1B4_320 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.179), at 376,354 to 377,628
_320
Pf1N1B4_321 - Probable short-chain dehydrogenase, at 377,661 to 378,455
_321
Position (kb) 376
377
378 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 375.543 kb on + strand at 375.609 kb on + strand at 375.609 kb on + strand at 375.609 kb on + strand at 375.609 kb on + strand at 375.609 kb on + strand at 375.609 kb on + strand at 375.609 kb on + strand at 375.609 kb on + strand at 375.609 kb on + strand at 375.610 kb on - strand at 375.610 kb on - strand at 375.687 kb on + strand at 375.687 kb on + strand at 375.688 kb on - strand at 375.688 kb on - strand at 375.688 kb on - strand at 375.743 kb on + strand, within Pf1N1B4_319 at 375.743 kb on + strand, within Pf1N1B4_319 at 375.743 kb on + strand, within Pf1N1B4_319 at 375.743 kb on + strand, within Pf1N1B4_319 at 375.743 kb on + strand, within Pf1N1B4_319 at 375.743 kb on + strand, within Pf1N1B4_319 at 375.744 kb on - strand, within Pf1N1B4_319 at 375.744 kb on - strand, within Pf1N1B4_319 at 375.744 kb on - strand, within Pf1N1B4_319 at 375.744 kb on - strand, within Pf1N1B4_319 at 375.744 kb on - strand, within Pf1N1B4_319 at 375.744 kb on - strand at 375.744 kb on - strand, within Pf1N1B4_319 at 376.084 kb on + strand, within Pf1N1B4_319 at 376.102 kb on - strand, within Pf1N1B4_319 at 376.102 kb on - strand, within Pf1N1B4_319 at 376.102 kb on - strand, within Pf1N1B4_319 at 376.168 kb on - strand, within Pf1N1B4_319 at 376.168 kb on - strand, within Pf1N1B4_319 at 376.168 kb on - strand, within Pf1N1B4_319 at 376.283 kb on - strand at 376.325 kb on + strand at 376.326 kb on - strand at 376.332 kb on + strand at 376.333 kb on - strand at 376.333 kb on - strand at 376.346 kb on - strand at 376.346 kb on - strand at 376.346 kb on - strand at 376.346 kb on - strand at 376.356 kb on - strand at 376.356 kb on - strand at 376.356 kb on - strand at 376.356 kb on - strand at 376.368 kb on - strand at 376.368 kb on - strand at 376.424 kb on + strand at 376.491 kb on + strand, within Pf1N1B4_320 at 376.500 kb on + strand at 376.501 kb on - strand, within Pf1N1B4_320 at 376.797 kb on - strand, within Pf1N1B4_320 at 376.797 kb on - strand, within Pf1N1B4_320 at 376.797 kb on - strand at 376.965 kb on - strand at 377.049 kb on + strand, within Pf1N1B4_320 at 377.049 kb on + strand, within Pf1N1B4_320 at 377.050 kb on - strand, within Pf1N1B4_320 at 377.050 kb on - strand, within Pf1N1B4_320 at 377.379 kb on + strand, within Pf1N1B4_320 at 377.380 kb on - strand, within Pf1N1B4_320 at 377.380 kb on - strand, within Pf1N1B4_320 at 377.380 kb on - strand, within Pf1N1B4_320 at 377.380 kb on - strand, within Pf1N1B4_320 at 377.638 kb on - strand at 377.638 kb on - strand at 377.638 kb on - strand at 377.669 kb on - strand at 377.691 kb on - strand at 377.691 kb on - strand at 377.691 kb on - strand at 377.726 kb on + strand at 377.726 kb on + strand at 377.727 kb on - strand at 377.885 kb on + strand, within Pf1N1B4_321 at 377.886 kb on - strand, within Pf1N1B4_321 at 377.971 kb on + strand at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand at 377.989 kb on + strand, within Pf1N1B4_321 at 377.990 kb on - strand, within Pf1N1B4_321 at 377.990 kb on - strand, within Pf1N1B4_321 at 377.990 kb on - strand, within Pf1N1B4_321 at 378.039 kb on - strand, within Pf1N1B4_321 at 378.186 kb on - strand, within Pf1N1B4_321 at 378.539 kb on + strand at 378.540 kb on - strand at 378.540 kb on - strand at 378.553 kb on + strand at 378.554 kb on - strand at 378.554 kb on - strand at 378.558 kb on + strand at 378.565 kb on - strand at 378.613 kb on - strand at 378.613 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 375,543 + +1.1 375,609 + +0.9 375,609 + +0.2 375,609 + -0.6 375,609 + +0.4 375,609 + -0.5 375,609 + +0.4 375,609 + +1.0 375,609 + +0.4 375,609 + +0.0 375,610 - -0.2 375,610 - -0.9 375,687 + -1.6 375,687 + -1.8 375,688 - -0.3 375,688 - -0.2 375,688 - +0.1 375,743 + Pf1N1B4_319 0.17 -1.1 375,743 + Pf1N1B4_319 0.17 -1.8 375,743 + Pf1N1B4_319 0.17 -1.6 375,743 + Pf1N1B4_319 0.17 -1.6 375,743 + Pf1N1B4_319 0.17 -0.5 375,743 + Pf1N1B4_319 0.17 -0.3 375,744 - Pf1N1B4_319 0.17 +0.6 375,744 - Pf1N1B4_319 0.17 -0.6 375,744 - Pf1N1B4_319 0.17 +0.9 375,744 - Pf1N1B4_319 0.17 -0.8 375,744 - Pf1N1B4_319 0.17 +1.5 375,744 - -0.2 375,744 - Pf1N1B4_319 0.17 +0.7 376,084 + Pf1N1B4_319 0.67 -1.2 376,102 - Pf1N1B4_319 0.70 -0.4 376,102 - Pf1N1B4_319 0.70 +0.1 376,102 - Pf1N1B4_319 0.70 -0.9 376,168 - Pf1N1B4_319 0.79 +0.0 376,168 - Pf1N1B4_319 0.79 -0.2 376,168 - Pf1N1B4_319 0.79 +1.1 376,283 - +0.4 376,325 + +0.2 376,326 - -0.2 376,332 + +0.6 376,333 - -1.6 376,333 - -0.3 376,346 - +0.9 376,346 - -0.6 376,346 - -2.0 376,346 - -0.0 376,356 - -2.0 376,356 - +1.1 376,356 - +0.6 376,356 - -1.2 376,368 - -1.5 376,368 - -0.2 376,424 + +1.4 376,491 + Pf1N1B4_320 0.11 +0.1 376,500 + +0.1 376,501 - Pf1N1B4_320 0.12 -1.1 376,797 - Pf1N1B4_320 0.35 -3.1 376,797 - Pf1N1B4_320 0.35 +0.2 376,797 - +1.1 376,965 - +0.4 377,049 + Pf1N1B4_320 0.55 +0.2 377,049 + Pf1N1B4_320 0.55 -1.4 377,050 - Pf1N1B4_320 0.55 +0.1 377,050 - Pf1N1B4_320 0.55 -1.5 377,379 + Pf1N1B4_320 0.80 -2.0 377,380 - Pf1N1B4_320 0.80 -2.6 377,380 - Pf1N1B4_320 0.80 -0.2 377,380 - Pf1N1B4_320 0.80 -2.2 377,380 - Pf1N1B4_320 0.80 -0.5 377,638 - +0.4 377,638 - +0.5 377,638 - -1.0 377,669 - -0.2 377,691 - -0.2 377,691 - -0.8 377,691 - +0.1 377,726 + -2.2 377,726 + -1.5 377,727 - +1.2 377,885 + Pf1N1B4_321 0.28 -0.9 377,886 - Pf1N1B4_321 0.28 -0.1 377,971 + -1.6 377,972 - Pf1N1B4_321 0.39 -0.0 377,972 - Pf1N1B4_321 0.39 +0.1 377,972 - Pf1N1B4_321 0.39 -0.5 377,972 - Pf1N1B4_321 0.39 -0.4 377,972 - Pf1N1B4_321 0.39 -0.9 377,972 - Pf1N1B4_321 0.39 -1.6 377,972 - Pf1N1B4_321 0.39 +0.7 377,972 - Pf1N1B4_321 0.39 -0.5 377,972 - Pf1N1B4_321 0.39 +0.1 377,972 - Pf1N1B4_321 0.39 +0.5 377,972 - Pf1N1B4_321 0.39 +0.9 377,972 - Pf1N1B4_321 0.39 -1.0 377,972 - Pf1N1B4_321 0.39 -0.1 377,972 - -0.5 377,989 + Pf1N1B4_321 0.41 -1.0 377,990 - Pf1N1B4_321 0.41 +0.4 377,990 - Pf1N1B4_321 0.41 -0.3 377,990 - Pf1N1B4_321 0.41 -0.4 378,039 - Pf1N1B4_321 0.48 -0.6 378,186 - Pf1N1B4_321 0.66 -0.3 378,539 + -1.2 378,540 - -0.7 378,540 - -0.3 378,553 + +0.6 378,554 - +0.1 378,554 - +1.4 378,558 + +0.0 378,565 - -0.4 378,613 - -0.9 378,613 - -0.9
Or see this region's nucleotide sequence