Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2790 and Pf1N1B4_2791 are separated by 160 nucleotides Pf1N1B4_2791 and Pf1N1B4_2792 are separated by 201 nucleotides Pf1N1B4_2792 and Pf1N1B4_2793 are separated by 130 nucleotides Pf1N1B4_2793 and Pf1N1B4_2794 are separated by 143 nucleotides
Pf1N1B4_2790 - FIG00960839: hypothetical protein, at 3,135,125 to 3,136,954
_2790
Pf1N1B4_2791 - Putative inner membrane protein (Fragment), at 3,137,115 to 3,137,603
_2791
Pf1N1B4_2792 - FIG00953454: hypothetical protein, at 3,137,805 to 3,138,101
_2792
Pf1N1B4_2793 - FKBP-type peptidyl-prolyl cis-trans isomerase FklB (EC 5.2.1.8), at 3,138,232 to 3,138,849
_2793
Pf1N1B4_2794 - FIG00953595: hypothetical protein, at 3,138,993 to 3,139,250
_2794
Position (kb)
3137
3138
3139 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3137.062 kb on + strand at 3137.062 kb on + strand at 3137.063 kb on - strand at 3137.077 kb on - strand at 3137.078 kb on + strand at 3137.078 kb on + strand at 3137.079 kb on - strand at 3137.142 kb on + strand at 3137.142 kb on + strand at 3137.142 kb on + strand at 3137.142 kb on + strand at 3137.142 kb on + strand at 3137.143 kb on - strand at 3137.143 kb on - strand at 3137.172 kb on + strand, within Pf1N1B4_2791 at 3137.173 kb on - strand, within Pf1N1B4_2791 at 3137.243 kb on + strand, within Pf1N1B4_2791 at 3137.529 kb on + strand, within Pf1N1B4_2791 at 3137.529 kb on + strand, within Pf1N1B4_2791 at 3137.529 kb on + strand, within Pf1N1B4_2791 at 3137.742 kb on + strand at 3137.742 kb on + strand at 3137.742 kb on + strand at 3137.742 kb on + strand at 3137.742 kb on + strand at 3137.743 kb on - strand at 3137.743 kb on - strand at 3137.743 kb on - strand at 3137.769 kb on + strand at 3137.769 kb on + strand at 3137.769 kb on + strand at 3137.769 kb on + strand at 3137.769 kb on + strand at 3137.769 kb on + strand at 3137.770 kb on - strand at 3137.770 kb on - strand at 3137.770 kb on - strand at 3137.770 kb on - strand at 3137.770 kb on - strand at 3137.829 kb on + strand at 3137.829 kb on + strand at 3137.829 kb on + strand at 3137.829 kb on + strand at 3137.830 kb on - strand at 3137.830 kb on - strand at 3137.830 kb on - strand at 3137.887 kb on - strand, within Pf1N1B4_2792 at 3137.947 kb on + strand, within Pf1N1B4_2792 at 3137.948 kb on - strand, within Pf1N1B4_2792 at 3137.948 kb on - strand, within Pf1N1B4_2792 at 3137.948 kb on - strand, within Pf1N1B4_2792 at 3137.948 kb on - strand, within Pf1N1B4_2792 at 3138.023 kb on + strand, within Pf1N1B4_2792 at 3138.023 kb on + strand, within Pf1N1B4_2792 at 3138.023 kb on + strand, within Pf1N1B4_2792 at 3138.023 kb on + strand, within Pf1N1B4_2792 at 3138.023 kb on + strand, within Pf1N1B4_2792 at 3138.023 kb on + strand, within Pf1N1B4_2792 at 3138.076 kb on + strand at 3138.142 kb on - strand at 3138.242 kb on + strand at 3138.243 kb on - strand at 3138.243 kb on - strand at 3138.611 kb on - strand, within Pf1N1B4_2793 at 3138.652 kb on + strand, within Pf1N1B4_2793 at 3138.653 kb on - strand, within Pf1N1B4_2793 at 3138.751 kb on + strand, within Pf1N1B4_2793 at 3138.751 kb on + strand, within Pf1N1B4_2793 at 3138.751 kb on + strand, within Pf1N1B4_2793 at 3138.751 kb on + strand, within Pf1N1B4_2793 at 3138.751 kb on + strand, within Pf1N1B4_2793 at 3138.752 kb on - strand at 3138.752 kb on - strand, within Pf1N1B4_2793 at 3138.752 kb on - strand, within Pf1N1B4_2793 at 3138.752 kb on - strand, within Pf1N1B4_2793 at 3138.752 kb on - strand, within Pf1N1B4_2793 at 3138.812 kb on + strand at 3138.813 kb on - strand at 3138.813 kb on - strand at 3138.818 kb on + strand at 3138.847 kb on + strand at 3138.848 kb on - strand at 3138.848 kb on - strand at 3138.997 kb on - strand at 3138.997 kb on - strand at 3138.997 kb on - strand at 3139.045 kb on + strand, within Pf1N1B4_2794 at 3139.046 kb on - strand, within Pf1N1B4_2794
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 3,137,062 + -0.0 3,137,062 + -1.9 3,137,063 - -0.2 3,137,077 - -0.9 3,137,078 + -1.8 3,137,078 + -0.5 3,137,079 - -0.1 3,137,142 + +0.7 3,137,142 + -0.2 3,137,142 + +1.4 3,137,142 + +3.0 3,137,142 + -1.3 3,137,143 - +0.3 3,137,143 - +0.7 3,137,172 + Pf1N1B4_2791 0.12 +0.4 3,137,173 - Pf1N1B4_2791 0.12 -0.3 3,137,243 + Pf1N1B4_2791 0.26 +1.1 3,137,529 + Pf1N1B4_2791 0.85 +0.2 3,137,529 + Pf1N1B4_2791 0.85 -1.9 3,137,529 + Pf1N1B4_2791 0.85 +0.4 3,137,742 + -0.2 3,137,742 + +1.5 3,137,742 + +0.4 3,137,742 + -2.6 3,137,742 + +0.1 3,137,743 - +0.1 3,137,743 - +0.6 3,137,743 - +1.4 3,137,769 + -1.1 3,137,769 + -0.3 3,137,769 + +1.1 3,137,769 + -0.3 3,137,769 + -0.4 3,137,769 + -0.5 3,137,770 - +1.4 3,137,770 - +1.0 3,137,770 - -0.9 3,137,770 - -2.0 3,137,770 - +0.0 3,137,829 + -0.8 3,137,829 + -0.5 3,137,829 + +1.4 3,137,829 + -1.0 3,137,830 - +0.1 3,137,830 - +1.3 3,137,830 - -0.6 3,137,887 - Pf1N1B4_2792 0.28 -1.6 3,137,947 + Pf1N1B4_2792 0.48 -0.6 3,137,948 - Pf1N1B4_2792 0.48 -2.6 3,137,948 - Pf1N1B4_2792 0.48 -0.4 3,137,948 - Pf1N1B4_2792 0.48 -0.5 3,137,948 - Pf1N1B4_2792 0.48 -0.4 3,138,023 + Pf1N1B4_2792 0.73 +0.4 3,138,023 + Pf1N1B4_2792 0.73 -0.8 3,138,023 + Pf1N1B4_2792 0.73 -0.7 3,138,023 + Pf1N1B4_2792 0.73 -1.8 3,138,023 + Pf1N1B4_2792 0.73 -1.8 3,138,023 + Pf1N1B4_2792 0.73 -1.9 3,138,076 + -0.0 3,138,142 - -0.7 3,138,242 + +2.7 3,138,243 - -2.4 3,138,243 - +0.7 3,138,611 - Pf1N1B4_2793 0.61 +1.1 3,138,652 + Pf1N1B4_2793 0.68 +0.8 3,138,653 - Pf1N1B4_2793 0.68 -0.9 3,138,751 + Pf1N1B4_2793 0.84 +0.1 3,138,751 + Pf1N1B4_2793 0.84 +0.1 3,138,751 + Pf1N1B4_2793 0.84 -1.3 3,138,751 + Pf1N1B4_2793 0.84 +0.7 3,138,751 + Pf1N1B4_2793 0.84 -0.2 3,138,752 - +0.8 3,138,752 - Pf1N1B4_2793 0.84 -0.3 3,138,752 - Pf1N1B4_2793 0.84 +1.4 3,138,752 - Pf1N1B4_2793 0.84 +1.6 3,138,752 - Pf1N1B4_2793 0.84 -2.6 3,138,812 + -1.2 3,138,813 - -1.2 3,138,813 - -1.0 3,138,818 + -0.6 3,138,847 + +1.8 3,138,848 - -1.2 3,138,848 - -0.6 3,138,997 - -0.2 3,138,997 - +0.6 3,138,997 - +0.2 3,139,045 + Pf1N1B4_2794 0.20 +0.4 3,139,046 - Pf1N1B4_2794 0.21 +1.8
Or see this region's nucleotide sequence