Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_235 and Pf1N1B4_236 are separated by 22 nucleotides Pf1N1B4_236 and Pf1N1B4_237 are separated by 81 nucleotides Pf1N1B4_237 and Pf1N1B4_238 are separated by 76 nucleotides Pf1N1B4_238 and Pf1N1B4_239 are separated by 89 nucleotides
Pf1N1B4_235 - Molybdopterin biosynthesis protein MoeA, at 295,218 to 296,405
_235
Pf1N1B4_236 - Molybdenum cofactor biosynthesis protein MoaB, at 296,428 to 296,967
_236
Pf1N1B4_237 - Molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 297,049 to 297,651
_237
Pf1N1B4_238 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 297,728 to 297,949
_238
Pf1N1B4_239 - Similar to ribosomal large subunit pseudouridine synthase A, at 298,039 to 298,929
_239
Position (kb) 297
298 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 296.053 kb on - strand, within Pf1N1B4_235 at 296.053 kb on - strand, within Pf1N1B4_235 at 296.148 kb on - strand, within Pf1N1B4_235 at 296.148 kb on - strand, within Pf1N1B4_235 at 296.240 kb on - strand, within Pf1N1B4_235 at 296.638 kb on + strand, within Pf1N1B4_236 at 296.821 kb on + strand, within Pf1N1B4_236 at 296.821 kb on + strand, within Pf1N1B4_236 at 296.821 kb on + strand, within Pf1N1B4_236 at 296.838 kb on + strand, within Pf1N1B4_236 at 296.884 kb on - strand, within Pf1N1B4_236 at 296.947 kb on + strand at 296.948 kb on - strand at 296.948 kb on - strand at 296.967 kb on + strand at 296.967 kb on + strand at 296.967 kb on + strand at 296.968 kb on - strand at 296.968 kb on - strand at 296.983 kb on + strand at 296.983 kb on + strand at 296.983 kb on + strand at 296.984 kb on - strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.004 kb on - strand at 297.004 kb on - strand at 297.005 kb on + strand at 297.005 kb on + strand at 297.005 kb on + strand at 297.006 kb on - strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.046 kb on - strand at 297.046 kb on - strand at 297.060 kb on + strand at 297.060 kb on + strand at 297.061 kb on - strand at 297.061 kb on - strand at 297.061 kb on - strand at 297.238 kb on + strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.342 kb on - strand, within Pf1N1B4_237 at 297.543 kb on + strand, within Pf1N1B4_237 at 297.543 kb on + strand, within Pf1N1B4_237 at 297.609 kb on + strand at 297.623 kb on + strand at 297.677 kb on + strand at 297.677 kb on + strand at 297.678 kb on - strand at 297.678 kb on - strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.680 kb on - strand at 297.800 kb on - strand at 297.947 kb on + strand at 297.970 kb on + strand at 297.970 kb on + strand at 297.988 kb on + strand at 298.011 kb on - strand at 298.011 kb on - strand at 298.140 kb on - strand, within Pf1N1B4_239 at 298.140 kb on - strand, within Pf1N1B4_239 at 298.140 kb on - strand, within Pf1N1B4_239 at 298.151 kb on - strand, within Pf1N1B4_239 at 298.251 kb on - strand, within Pf1N1B4_239 at 298.360 kb on - strand, within Pf1N1B4_239 at 298.372 kb on - strand, within Pf1N1B4_239
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 296,053 - Pf1N1B4_235 0.70 -0.8 296,053 - Pf1N1B4_235 0.70 +0.4 296,148 - Pf1N1B4_235 0.78 -2.1 296,148 - Pf1N1B4_235 0.78 -0.1 296,240 - Pf1N1B4_235 0.86 +0.8 296,638 + Pf1N1B4_236 0.39 -0.1 296,821 + Pf1N1B4_236 0.73 +0.9 296,821 + Pf1N1B4_236 0.73 +0.0 296,821 + Pf1N1B4_236 0.73 -1.6 296,838 + Pf1N1B4_236 0.76 -0.4 296,884 - Pf1N1B4_236 0.84 -2.4 296,947 + -0.8 296,948 - +0.2 296,948 - -1.0 296,967 + -0.9 296,967 + -2.0 296,967 + -0.6 296,968 - -0.8 296,968 - -2.1 296,983 + -0.6 296,983 + -1.0 296,983 + +0.5 296,984 - -0.1 297,003 + +0.4 297,003 + -0.6 297,003 + -0.2 297,003 + +0.0 297,003 + +0.2 297,003 + +1.7 297,003 + -0.9 297,003 + -0.0 297,003 + -0.4 297,004 - -0.2 297,004 - +0.3 297,005 + +0.1 297,005 + -0.3 297,005 + -2.3 297,006 - -1.1 297,045 + +0.6 297,045 + +1.4 297,045 + -1.0 297,045 + -1.8 297,045 + -0.8 297,046 - -2.2 297,046 - +0.4 297,060 + -0.6 297,060 + -1.0 297,061 - -0.6 297,061 - -1.0 297,061 - -0.3 297,238 + Pf1N1B4_237 0.31 -1.0 297,239 - Pf1N1B4_237 0.32 -1.4 297,239 - Pf1N1B4_237 0.32 -0.9 297,239 - Pf1N1B4_237 0.32 -0.5 297,341 + Pf1N1B4_237 0.48 -1.2 297,341 + Pf1N1B4_237 0.48 -1.0 297,341 + Pf1N1B4_237 0.48 +0.1 297,342 - Pf1N1B4_237 0.49 -1.0 297,543 + Pf1N1B4_237 0.82 +0.4 297,543 + Pf1N1B4_237 0.82 -3.3 297,609 + -1.6 297,623 + +1.7 297,677 + +1.4 297,677 + +0.8 297,678 - -0.4 297,678 - -0.5 297,679 + +0.6 297,679 + +0.8 297,679 + -1.0 297,679 + -0.6 297,679 + -2.2 297,679 + +0.4 297,679 + +1.4 297,679 + -1.4 297,680 - -0.4 297,800 - -0.6 297,947 + -0.1 297,970 + -0.3 297,970 + +0.6 297,988 + -0.2 298,011 - +1.4 298,011 - -0.1 298,140 - Pf1N1B4_239 0.11 -1.0 298,140 - Pf1N1B4_239 0.11 +0.9 298,140 - Pf1N1B4_239 0.11 +0.8 298,151 - Pf1N1B4_239 0.13 -1.3 298,251 - Pf1N1B4_239 0.24 +0.8 298,360 - Pf1N1B4_239 0.36 -0.2 298,372 - Pf1N1B4_239 0.37 -2.5
Or see this region's nucleotide sequence