Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1953

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
2,233,555	+		Pf1N1B4_1952	0.53	-0.4
2,233,556	-		Pf1N1B4_1952	0.53	-0.2
2,233,776	+		Pf1N1B4_1952	0.65	-0.2
2,233,776	+		Pf1N1B4_1952	0.65	-0.7
2,233,776	+		Pf1N1B4_1952	0.65	-0.9
2,233,776	+				-0.9
2,233,776	+		Pf1N1B4_1952	0.65	+0.5
2,233,776	+		Pf1N1B4_1952	0.65	+0.2
2,233,776	+		Pf1N1B4_1952	0.65	-0.4
2,233,776	+		Pf1N1B4_1952	0.65	-0.9
2,233,777	-		Pf1N1B4_1952	0.65	-0.2
2,233,777	-		Pf1N1B4_1952	0.65	+0.0
2,233,777	-		Pf1N1B4_1952	0.65	+0.7
2,233,777	-		Pf1N1B4_1952	0.65	-1.8
2,233,777	-		Pf1N1B4_1952	0.65	-0.4
2,233,834	-		Pf1N1B4_1952	0.68	-0.6
2,233,834	-		Pf1N1B4_1952	0.68	+2.0
2,233,877	+				+0.4
2,233,877	+		Pf1N1B4_1952	0.71	-0.9
2,233,877	+		Pf1N1B4_1952	0.71	-0.2
2,233,877	+		Pf1N1B4_1952	0.71	-0.2
2,233,877	+		Pf1N1B4_1952	0.71	+0.6
2,233,877	+		Pf1N1B4_1952	0.71	-1.4
2,233,877	+		Pf1N1B4_1952	0.71	-0.3
2,233,878	-		Pf1N1B4_1952	0.71	-0.5
2,233,878	-		Pf1N1B4_1952	0.71	-0.2
2,233,878	-		Pf1N1B4_1952	0.71	-1.0
2,233,878	-		Pf1N1B4_1952	0.71	+1.4
2,233,878	-		Pf1N1B4_1952	0.71	+0.9
2,233,878	-		Pf1N1B4_1952	0.71	+1.4
2,233,881	+		Pf1N1B4_1952	0.71	-0.6
2,233,881	+		Pf1N1B4_1952	0.71	-0.2
2,233,881	+		Pf1N1B4_1952	0.71	-1.2
2,233,881	+		Pf1N1B4_1952	0.71	-0.5
2,233,881	+		Pf1N1B4_1952	0.71	+1.9
2,233,881	+		Pf1N1B4_1952	0.71	-1.0
2,233,950	+		Pf1N1B4_1952	0.75	-0.6
2,233,950	+		Pf1N1B4_1952	0.75	-1.3
2,233,950	+		Pf1N1B4_1952	0.75	+0.4
2,233,951	-		Pf1N1B4_1952	0.75	+0.4
2,233,951	-		Pf1N1B4_1952	0.75	-0.5
2,233,951	-		Pf1N1B4_1952	0.75	+0.5
2,234,007	+		Pf1N1B4_1952	0.78	+1.4
2,234,007	+		Pf1N1B4_1952	0.78	+1.4
2,234,007	+		Pf1N1B4_1952	0.78	-0.6
2,234,007	+		Pf1N1B4_1952	0.78	-0.1
2,234,008	-		Pf1N1B4_1952	0.78	+1.1
2,234,008	-		Pf1N1B4_1952	0.78	-0.3
2,234,334	+				-0.5
2,234,371	+				-0.9
2,234,372	-				+1.1
2,234,372	-				+1.2
2,234,401	+				-0.4
2,234,417	+				-1.3
2,234,417	+				+1.4
2,234,418	-				+0.7
2,234,418	-				+0.9
2,234,418	-				-0.4
2,234,431	+				+0.4
2,234,431	+				-0.1
2,234,432	-				-0.5
2,234,432	-				-2.2
2,234,494	+		Pf1N1B4_1953	0.10	-2.1
2,234,495	-		Pf1N1B4_1953	0.10	-0.7
2,234,495	-		Pf1N1B4_1953	0.10	+0.4
2,234,495	-		Pf1N1B4_1953	0.10	-0.5
2,234,495	-		Pf1N1B4_1953	0.10	-1.2
2,234,495	-		Pf1N1B4_1953	0.10	-1.8
2,234,586	+		Pf1N1B4_1953	0.25	-1.5
2,234,587	-		Pf1N1B4_1953	0.25	-0.6
2,234,623	+		Pf1N1B4_1953	0.31	-0.9
2,234,623	+		Pf1N1B4_1953	0.31	+1.1
2,234,624	-		Pf1N1B4_1953	0.31	+1.6
2,234,665	+		Pf1N1B4_1953	0.38	+0.1
2,234,666	-		Pf1N1B4_1953	0.38	-1.2
2,234,666	-		Pf1N1B4_1953	0.38	-1.4
2,234,780	+		Pf1N1B4_1953	0.56	-0.4
2,234,785	+		Pf1N1B4_1953	0.57	+1.2
2,234,878	-		Pf1N1B4_1953	0.72	-1.1
2,234,886	+		Pf1N1B4_1953	0.73	-0.6
2,234,886	+		Pf1N1B4_1953	0.73	+1.0
2,235,032	+				-1.2
2,235,032	+				-0.4
2,235,032	+				-0.6
2,235,032	+				-0.2
2,235,032	+				+1.1
2,235,032	+				-1.4
2,235,033	-				+0.1
2,235,033	-				+0.8
2,235,033	-				+1.1
2,235,033	-				-1.9
2,235,033	-				-0.6
2,235,040	+				-0.6
2,235,040	+				+0.6
2,235,040	+				+0.2
2,235,040	+				+1.5
2,235,040	+				-2.3
2,235,041	-				-0.4
2,235,041	-				-0.0
2,235,041	-				-1.2
2,235,041	-				+0.2
2,235,041	-				+1.4
2,235,041	-				-1.1
2,235,041	-				-0.5
2,235,096	+				+1.4
2,235,097	-				-0.4
2,235,098	+				-1.5
2,235,098	+				-0.0
2,235,098	+				+1.1
2,235,098	+				-0.8
2,235,098	+				-0.7
2,235,098	+				+0.1
2,235,098	+				-1.2
2,235,098	+				-0.2
2,235,098	+				-0.4
2,235,098	+				-0.2
2,235,098	+				-0.4
2,235,098	+				+0.4
2,235,098	+				+0.4
2,235,098	+				-0.7
2,235,098	+				+1.4
2,235,098	+				-1.2
2,235,099	-				+0.2
2,235,099	-				-0.8
2,235,099	-				-1.8
2,235,099	-				-1.2
2,235,099	-				+0.2
2,235,099	-				+0.9
2,235,099	-				+0.7
2,235,099	-				+1.0
2,235,099	-				+1.0
2,235,099	-				-0.3
2,235,099	-				+0.5
2,235,099	-				-1.2
2,235,099	-				+0.3
2,235,099	-				-0.3
2,235,099	-				-2.4
2,235,099	-				-0.2
2,235,099	-				+0.2
2,235,099	-				+1.7
2,235,099	-				+0.2
2,235,099	-				+0.8
2,235,099	-				+1.4
2,235,099	-				+0.3
2,235,108	+				-2.4
2,235,108	+				-1.2
2,235,123	+				-1.2
2,235,124	-				+1.7
2,235,207	+				-1.0
2,235,208	-				+0.1
2,235,208	-				+1.1
2,235,365	+		Pf1N1B4_1954	0.17	-0.1
2,235,365	+		Pf1N1B4_1954	0.17	+0.4
2,235,365	+		Pf1N1B4_1954	0.17	+0.1
2,235,365	+		Pf1N1B4_1954	0.17	+1.0
2,235,365	+		Pf1N1B4_1954	0.17	+0.5
2,235,365	+		Pf1N1B4_1954	0.17	-0.4
2,235,365	+		Pf1N1B4_1954	0.17	-2.9
2,235,366	-		Pf1N1B4_1954	0.17	-0.0
2,235,366	-		Pf1N1B4_1954	0.17	+1.7
2,235,366	-		Pf1N1B4_1954	0.17	+3.0
2,235,366	-		Pf1N1B4_1954	0.17	-0.1
2,235,366	-		Pf1N1B4_1954	0.17	+0.2
2,235,366	-		Pf1N1B4_1954	0.17	-1.3
2,235,366	-		Pf1N1B4_1954	0.17	+1.0
2,235,552	+		Pf1N1B4_1954	0.38	-1.5
2,235,553	-		Pf1N1B4_1954	0.38	-0.9
2,235,553	-		Pf1N1B4_1954	0.38	-1.5
2,235,553	-		Pf1N1B4_1954	0.38	-0.2
2,235,937	-		Pf1N1B4_1954	0.80	-0.9
2,235,937	-		Pf1N1B4_1954	0.80	-1.4