Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1805 and Pf1N1B4_1806 overlap by 4 nucleotides Pf1N1B4_1806 and Pf1N1B4_1807 are separated by 10 nucleotides
Pf1N1B4_1805 - PROBABLE REMNANT OF A TRANSPOSASE GENE PROTEIN, at 2,072,919 to 2,073,695
_1805
Pf1N1B4_1806 - FIG00953743: hypothetical protein, at 2,073,692 to 2,074,624
_1806
Pf1N1B4_1807 - Non-ribosomal peptide synthetase modules and related proteins, at 2,074,635 to 2,078,069
_1807
Position (kb)
2073
2074
2075 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2072.761 kb on - strand at 2073.189 kb on + strand, within Pf1N1B4_1805 at 2073.190 kb on - strand, within Pf1N1B4_1805 at 2073.210 kb on + strand, within Pf1N1B4_1805 at 2073.210 kb on + strand, within Pf1N1B4_1805 at 2073.210 kb on + strand, within Pf1N1B4_1805 at 2073.210 kb on + strand, within Pf1N1B4_1805 at 2073.210 kb on + strand, within Pf1N1B4_1805 at 2073.211 kb on - strand, within Pf1N1B4_1805 at 2073.493 kb on - strand, within Pf1N1B4_1805 at 2073.495 kb on + strand, within Pf1N1B4_1805 at 2073.495 kb on + strand, within Pf1N1B4_1805 at 2073.495 kb on + strand, within Pf1N1B4_1805 at 2073.495 kb on + strand, within Pf1N1B4_1805 at 2073.496 kb on - strand, within Pf1N1B4_1805 at 2073.496 kb on - strand, within Pf1N1B4_1805 at 2073.509 kb on + strand, within Pf1N1B4_1805 at 2073.510 kb on - strand, within Pf1N1B4_1805 at 2073.885 kb on - strand, within Pf1N1B4_1806 at 2074.015 kb on - strand, within Pf1N1B4_1806 at 2074.015 kb on - strand, within Pf1N1B4_1806 at 2074.015 kb on - strand, within Pf1N1B4_1806 at 2074.025 kb on + strand, within Pf1N1B4_1806 at 2074.401 kb on - strand, within Pf1N1B4_1806 at 2074.401 kb on - strand, within Pf1N1B4_1806 at 2074.509 kb on - strand, within Pf1N1B4_1806 at 2074.509 kb on - strand, within Pf1N1B4_1806 at 2074.610 kb on + strand at 2074.823 kb on - strand at 2074.823 kb on - strand at 2074.823 kb on - strand at 2075.244 kb on + strand, within Pf1N1B4_1807 at 2075.367 kb on + strand, within Pf1N1B4_1807 at 2075.367 kb on + strand, within Pf1N1B4_1807 at 2075.368 kb on - strand, within Pf1N1B4_1807 at 2075.406 kb on + strand, within Pf1N1B4_1807 at 2075.406 kb on + strand, within Pf1N1B4_1807 at 2075.406 kb on + strand, within Pf1N1B4_1807 at 2075.406 kb on + strand, within Pf1N1B4_1807 at 2075.619 kb on + strand, within Pf1N1B4_1807 at 2075.619 kb on + strand, within Pf1N1B4_1807 at 2075.619 kb on + strand, within Pf1N1B4_1807 at 2075.619 kb on + strand, within Pf1N1B4_1807 at 2075.619 kb on + strand, within Pf1N1B4_1807 at 2075.619 kb on + strand, within Pf1N1B4_1807 at 2075.620 kb on - strand, within Pf1N1B4_1807
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 2,072,761 - +0.5 2,073,189 + Pf1N1B4_1805 0.35 -0.6 2,073,190 - Pf1N1B4_1805 0.35 +0.1 2,073,210 + Pf1N1B4_1805 0.37 +0.1 2,073,210 + Pf1N1B4_1805 0.37 +0.8 2,073,210 + Pf1N1B4_1805 0.37 +1.7 2,073,210 + Pf1N1B4_1805 0.37 -0.2 2,073,210 + Pf1N1B4_1805 0.37 +0.1 2,073,211 - Pf1N1B4_1805 0.38 -0.6 2,073,493 - Pf1N1B4_1805 0.74 -0.3 2,073,495 + Pf1N1B4_1805 0.74 -0.3 2,073,495 + Pf1N1B4_1805 0.74 +1.0 2,073,495 + Pf1N1B4_1805 0.74 -0.4 2,073,495 + Pf1N1B4_1805 0.74 -0.9 2,073,496 - Pf1N1B4_1805 0.74 +1.0 2,073,496 - Pf1N1B4_1805 0.74 -0.9 2,073,509 + Pf1N1B4_1805 0.76 -1.4 2,073,510 - Pf1N1B4_1805 0.76 -0.0 2,073,885 - Pf1N1B4_1806 0.21 -0.2 2,074,015 - Pf1N1B4_1806 0.35 -1.9 2,074,015 - Pf1N1B4_1806 0.35 -1.4 2,074,015 - Pf1N1B4_1806 0.35 -0.5 2,074,025 + Pf1N1B4_1806 0.36 +1.4 2,074,401 - Pf1N1B4_1806 0.76 -1.5 2,074,401 - Pf1N1B4_1806 0.76 -3.3 2,074,509 - Pf1N1B4_1806 0.88 -0.3 2,074,509 - Pf1N1B4_1806 0.88 -0.9 2,074,610 + +0.8 2,074,823 - +1.6 2,074,823 - +0.7 2,074,823 - -0.3 2,075,244 + Pf1N1B4_1807 0.18 +1.7 2,075,367 + Pf1N1B4_1807 0.21 +0.0 2,075,367 + Pf1N1B4_1807 0.21 +1.4 2,075,368 - Pf1N1B4_1807 0.21 -3.2 2,075,406 + Pf1N1B4_1807 0.22 -1.7 2,075,406 + Pf1N1B4_1807 0.22 +0.4 2,075,406 + Pf1N1B4_1807 0.22 -0.6 2,075,406 + Pf1N1B4_1807 0.22 +0.2 2,075,619 + Pf1N1B4_1807 0.29 -0.5 2,075,619 + Pf1N1B4_1807 0.29 -1.5 2,075,619 + Pf1N1B4_1807 0.29 -0.7 2,075,619 + Pf1N1B4_1807 0.29 +0.5 2,075,619 + Pf1N1B4_1807 0.29 +1.4 2,075,619 + Pf1N1B4_1807 0.29 -1.0 2,075,620 - Pf1N1B4_1807 0.29 -0.2
Or see this region's nucleotide sequence