Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1781 and Pf1N1B4_1782 are separated by 322 nucleotides Pf1N1B4_1782 and Pf1N1B4_1783 are separated by 78 nucleotides
Pf1N1B4_1781 - hypothetical protein, at 2,034,706 to 2,035,005
_1781
Pf1N1B4_1782 - hypothetical protein, at 2,035,328 to 2,035,510
_1782
Pf1N1B4_1783 - hypothetical protein, at 2,035,589 to 2,036,581
_1783
Position (kb)
2035
2036 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2034.349 kb on - strand at 2034.349 kb on - strand at 2034.349 kb on - strand at 2034.368 kb on + strand at 2034.452 kb on + strand at 2034.452 kb on + strand at 2034.452 kb on + strand at 2034.453 kb on - strand at 2034.517 kb on + strand at 2034.517 kb on + strand at 2034.517 kb on + strand at 2034.517 kb on + strand at 2034.567 kb on + strand at 2034.783 kb on - strand, within Pf1N1B4_1781 at 2034.967 kb on + strand, within Pf1N1B4_1781 at 2034.967 kb on + strand, within Pf1N1B4_1781 at 2034.968 kb on - strand, within Pf1N1B4_1781 at 2034.999 kb on + strand at 2034.999 kb on + strand at 2034.999 kb on + strand at 2034.999 kb on + strand at 2035.000 kb on - strand at 2035.000 kb on - strand at 2035.000 kb on - strand at 2035.008 kb on + strand at 2035.008 kb on + strand at 2035.008 kb on + strand at 2035.008 kb on + strand at 2035.008 kb on + strand at 2035.008 kb on + strand at 2035.008 kb on + strand at 2035.009 kb on - strand at 2035.009 kb on - strand at 2035.012 kb on + strand at 2035.012 kb on + strand at 2035.013 kb on - strand at 2035.013 kb on - strand at 2035.013 kb on - strand at 2035.096 kb on + strand at 2035.097 kb on - strand at 2035.097 kb on - strand at 2035.097 kb on - strand at 2035.097 kb on - strand at 2035.097 kb on - strand at 2035.097 kb on - strand at 2035.100 kb on + strand at 2035.100 kb on + strand at 2035.100 kb on + strand at 2035.101 kb on - strand at 2035.101 kb on - strand at 2035.101 kb on - strand at 2035.101 kb on - strand at 2035.101 kb on - strand at 2035.101 kb on - strand at 2035.101 kb on - strand at 2035.101 kb on - strand at 2035.117 kb on - strand at 2035.141 kb on + strand at 2035.141 kb on + strand at 2035.141 kb on + strand at 2035.142 kb on - strand at 2035.142 kb on - strand at 2035.142 kb on - strand at 2035.142 kb on - strand at 2035.143 kb on + strand at 2035.143 kb on + strand at 2035.143 kb on + strand at 2035.144 kb on - strand at 2035.144 kb on - strand at 2035.144 kb on - strand at 2035.144 kb on - strand at 2035.144 kb on - strand at 2035.173 kb on + strand at 2035.173 kb on + strand at 2035.173 kb on + strand at 2035.173 kb on + strand at 2035.174 kb on - strand at 2035.174 kb on - strand at 2035.174 kb on - strand at 2035.174 kb on - strand at 2035.174 kb on - strand at 2035.174 kb on - strand at 2035.174 kb on - strand at 2035.174 kb on - strand at 2035.174 kb on - strand at 2035.188 kb on + strand at 2035.189 kb on - strand at 2035.189 kb on - strand at 2035.193 kb on - strand at 2035.193 kb on - strand at 2035.193 kb on - strand at 2035.193 kb on - strand at 2035.248 kb on + strand at 2035.249 kb on - strand at 2035.249 kb on - strand at 2035.249 kb on - strand at 2035.249 kb on - strand at 2035.249 kb on - strand at 2035.249 kb on - strand at 2035.249 kb on - strand at 2035.249 kb on - strand at 2035.249 kb on - strand at 2035.249 kb on - strand at 2035.273 kb on - strand at 2035.297 kb on - strand at 2035.297 kb on - strand at 2035.400 kb on - strand, within Pf1N1B4_1782 at 2035.456 kb on - strand, within Pf1N1B4_1782 at 2035.456 kb on - strand, within Pf1N1B4_1782 at 2035.456 kb on - strand, within Pf1N1B4_1782 at 2035.456 kb on - strand, within Pf1N1B4_1782 at 2035.524 kb on + strand at 2035.524 kb on + strand at 2035.524 kb on + strand at 2035.524 kb on + strand at 2035.525 kb on - strand at 2035.525 kb on - strand at 2035.525 kb on - strand at 2035.525 kb on - strand at 2035.597 kb on + strand at 2035.597 kb on + strand at 2035.597 kb on + strand at 2035.597 kb on + strand at 2035.597 kb on + strand at 2035.597 kb on + strand at 2035.597 kb on + strand at 2035.597 kb on + strand at 2035.598 kb on - strand at 2035.598 kb on - strand at 2035.598 kb on - strand at 2035.598 kb on - strand at 2035.598 kb on - strand at 2035.598 kb on - strand at 2035.598 kb on - strand at 2035.598 kb on - strand at 2035.598 kb on - strand at 2035.598 kb on - strand at 2035.598 kb on - strand at 2035.598 kb on - strand at 2035.598 kb on - strand at 2035.598 kb on - strand at 2035.605 kb on + strand at 2035.651 kb on + strand at 2035.651 kb on + strand at 2035.651 kb on + strand at 2035.651 kb on + strand at 2035.652 kb on - strand at 2035.652 kb on - strand at 2035.652 kb on - strand at 2035.652 kb on - strand at 2035.652 kb on - strand at 2035.653 kb on + strand at 2035.653 kb on + strand at 2035.653 kb on + strand at 2035.653 kb on + strand at 2035.653 kb on + strand at 2035.654 kb on - strand at 2035.654 kb on - strand at 2035.654 kb on - strand at 2035.654 kb on - strand at 2035.654 kb on - strand at 2035.654 kb on - strand at 2035.707 kb on + strand, within Pf1N1B4_1783 at 2035.707 kb on + strand, within Pf1N1B4_1783 at 2035.737 kb on + strand, within Pf1N1B4_1783 at 2035.737 kb on + strand, within Pf1N1B4_1783 at 2035.737 kb on + strand, within Pf1N1B4_1783 at 2035.738 kb on - strand, within Pf1N1B4_1783 at 2035.743 kb on + strand, within Pf1N1B4_1783 at 2035.743 kb on + strand, within Pf1N1B4_1783 at 2035.743 kb on + strand, within Pf1N1B4_1783 at 2035.743 kb on + strand, within Pf1N1B4_1783 at 2035.743 kb on + strand, within Pf1N1B4_1783 at 2035.744 kb on - strand, within Pf1N1B4_1783 at 2035.744 kb on - strand, within Pf1N1B4_1783 at 2035.744 kb on - strand, within Pf1N1B4_1783 at 2035.744 kb on - strand, within Pf1N1B4_1783 at 2035.749 kb on + strand, within Pf1N1B4_1783 at 2035.749 kb on + strand, within Pf1N1B4_1783 at 2035.749 kb on + strand, within Pf1N1B4_1783 at 2035.749 kb on + strand, within Pf1N1B4_1783 at 2035.749 kb on + strand, within Pf1N1B4_1783 at 2035.749 kb on + strand, within Pf1N1B4_1783 at 2035.749 kb on + strand, within Pf1N1B4_1783 at 2035.750 kb on - strand, within Pf1N1B4_1783 at 2035.750 kb on - strand, within Pf1N1B4_1783 at 2035.750 kb on - strand, within Pf1N1B4_1783 at 2035.750 kb on - strand, within Pf1N1B4_1783 at 2035.750 kb on - strand, within Pf1N1B4_1783 at 2035.750 kb on - strand, within Pf1N1B4_1783 at 2035.750 kb on - strand, within Pf1N1B4_1783 at 2035.750 kb on - strand, within Pf1N1B4_1783 at 2035.750 kb on - strand, within Pf1N1B4_1783 at 2035.768 kb on - strand, within Pf1N1B4_1783 at 2035.768 kb on - strand, within Pf1N1B4_1783 at 2035.980 kb on + strand, within Pf1N1B4_1783 at 2035.980 kb on + strand, within Pf1N1B4_1783 at 2035.980 kb on + strand, within Pf1N1B4_1783 at 2035.980 kb on + strand, within Pf1N1B4_1783 at 2036.056 kb on - strand, within Pf1N1B4_1783 at 2036.063 kb on + strand, within Pf1N1B4_1783 at 2036.063 kb on + strand, within Pf1N1B4_1783 at 2036.064 kb on - strand, within Pf1N1B4_1783 at 2036.064 kb on - strand, within Pf1N1B4_1783 at 2036.068 kb on - strand, within Pf1N1B4_1783 at 2036.068 kb on - strand, within Pf1N1B4_1783 at 2036.068 kb on - strand, within Pf1N1B4_1783 at 2036.146 kb on + strand, within Pf1N1B4_1783 at 2036.146 kb on + strand, within Pf1N1B4_1783 at 2036.147 kb on - strand, within Pf1N1B4_1783 at 2036.165 kb on - strand, within Pf1N1B4_1783 at 2036.210 kb on - strand, within Pf1N1B4_1783 at 2036.241 kb on - strand, within Pf1N1B4_1783 at 2036.241 kb on - strand, within Pf1N1B4_1783 at 2036.278 kb on + strand, within Pf1N1B4_1783 at 2036.326 kb on - strand, within Pf1N1B4_1783 at 2036.329 kb on + strand, within Pf1N1B4_1783 at 2036.329 kb on + strand, within Pf1N1B4_1783 at 2036.330 kb on - strand, within Pf1N1B4_1783 at 2036.330 kb on - strand, within Pf1N1B4_1783 at 2036.330 kb on - strand, within Pf1N1B4_1783 at 2036.331 kb on + strand, within Pf1N1B4_1783 at 2036.332 kb on - strand, within Pf1N1B4_1783 at 2036.332 kb on - strand, within Pf1N1B4_1783 at 2036.332 kb on - strand, within Pf1N1B4_1783 at 2036.364 kb on - strand, within Pf1N1B4_1783 at 2036.403 kb on + strand, within Pf1N1B4_1783 at 2036.403 kb on + strand, within Pf1N1B4_1783 at 2036.404 kb on - strand, within Pf1N1B4_1783 at 2036.404 kb on - strand, within Pf1N1B4_1783 at 2036.404 kb on - strand, within Pf1N1B4_1783
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 2,034,349 - +0.1 2,034,349 - +0.1 2,034,349 - -0.6 2,034,368 + -2.1 2,034,452 + +0.7 2,034,452 + +0.7 2,034,452 + -1.4 2,034,453 - -1.2 2,034,517 + +0.1 2,034,517 + -1.0 2,034,517 + -1.9 2,034,517 + -0.6 2,034,567 + +0.2 2,034,783 - Pf1N1B4_1781 0.26 -1.2 2,034,967 + Pf1N1B4_1781 0.87 -0.3 2,034,967 + Pf1N1B4_1781 0.87 -1.2 2,034,968 - Pf1N1B4_1781 0.87 -0.3 2,034,999 + -1.1 2,034,999 + -2.1 2,034,999 + +0.4 2,034,999 + +2.1 2,035,000 - +1.4 2,035,000 - -0.8 2,035,000 - +2.4 2,035,008 + +1.4 2,035,008 + -0.3 2,035,008 + -1.7 2,035,008 + -0.2 2,035,008 + -0.6 2,035,008 + +0.8 2,035,008 + +3.0 2,035,009 - +0.4 2,035,009 - -1.2 2,035,012 + -0.3 2,035,012 + +0.8 2,035,013 - -1.2 2,035,013 - -1.2 2,035,013 - -0.2 2,035,096 + +0.4 2,035,097 - -0.2 2,035,097 - +0.8 2,035,097 - -0.4 2,035,097 - -1.8 2,035,097 - +0.4 2,035,097 - +0.9 2,035,100 + -1.1 2,035,100 + +0.3 2,035,100 + +0.4 2,035,101 - -0.5 2,035,101 - -0.2 2,035,101 - +0.2 2,035,101 - -1.0 2,035,101 - -0.6 2,035,101 - -1.4 2,035,101 - -0.1 2,035,101 - -1.2 2,035,117 - +1.6 2,035,141 + -0.2 2,035,141 + +3.4 2,035,141 + -3.0 2,035,142 - +0.1 2,035,142 - -0.5 2,035,142 - -0.4 2,035,142 - +0.4 2,035,143 + -0.5 2,035,143 + +0.4 2,035,143 + -0.4 2,035,144 - -1.4 2,035,144 - -0.3 2,035,144 - +1.0 2,035,144 - -0.2 2,035,144 - +0.4 2,035,173 + +1.0 2,035,173 + -0.2 2,035,173 + +1.1 2,035,173 + +0.2 2,035,174 - -0.8 2,035,174 - +0.1 2,035,174 - -0.1 2,035,174 - -0.8 2,035,174 - +2.1 2,035,174 - +1.4 2,035,174 - -2.6 2,035,174 - +0.2 2,035,174 - -0.4 2,035,188 + -1.4 2,035,189 - +0.3 2,035,189 - -0.4 2,035,193 - +1.7 2,035,193 - -1.6 2,035,193 - +0.6 2,035,193 - -1.4 2,035,248 + -0.6 2,035,249 - +0.6 2,035,249 - +0.6 2,035,249 - -0.6 2,035,249 - -0.6 2,035,249 - +1.4 2,035,249 - +1.0 2,035,249 - -1.4 2,035,249 - +0.1 2,035,249 - -0.9 2,035,249 - -0.8 2,035,273 - -1.3 2,035,297 - +0.0 2,035,297 - -0.6 2,035,400 - Pf1N1B4_1782 0.39 -0.3 2,035,456 - Pf1N1B4_1782 0.70 +0.2 2,035,456 - Pf1N1B4_1782 0.70 -2.8 2,035,456 - Pf1N1B4_1782 0.70 -0.6 2,035,456 - Pf1N1B4_1782 0.70 -0.6 2,035,524 + -0.3 2,035,524 + -0.3 2,035,524 + +0.4 2,035,524 + -1.2 2,035,525 - -0.5 2,035,525 - +0.1 2,035,525 - +0.2 2,035,525 - -0.5 2,035,597 + -1.9 2,035,597 + -1.8 2,035,597 + +1.4 2,035,597 + +0.1 2,035,597 + -1.3 2,035,597 + -0.2 2,035,597 + +0.4 2,035,597 + -0.6 2,035,598 - +1.4 2,035,598 - +0.4 2,035,598 - -0.7 2,035,598 - +0.3 2,035,598 - -0.0 2,035,598 - -0.9 2,035,598 - +2.2 2,035,598 - +1.4 2,035,598 - +0.8 2,035,598 - +0.6 2,035,598 - +0.5 2,035,598 - -0.2 2,035,598 - +0.2 2,035,598 - -0.6 2,035,605 + -1.9 2,035,651 + -0.4 2,035,651 + +0.3 2,035,651 + -0.2 2,035,651 + -0.3 2,035,652 - +0.1 2,035,652 - -1.2 2,035,652 - +0.1 2,035,652 - -0.7 2,035,652 - +1.8 2,035,653 + -0.1 2,035,653 + +0.4 2,035,653 + +0.1 2,035,653 + +0.6 2,035,653 + +1.4 2,035,654 - +1.7 2,035,654 - -0.4 2,035,654 - -0.2 2,035,654 - -1.8 2,035,654 - -0.6 2,035,654 - -1.8 2,035,707 + Pf1N1B4_1783 0.12 -2.3 2,035,707 + Pf1N1B4_1783 0.12 -1.2 2,035,737 + Pf1N1B4_1783 0.15 -1.1 2,035,737 + Pf1N1B4_1783 0.15 +1.7 2,035,737 + Pf1N1B4_1783 0.15 -0.9 2,035,738 - Pf1N1B4_1783 0.15 -0.2 2,035,743 + Pf1N1B4_1783 0.16 -0.0 2,035,743 + Pf1N1B4_1783 0.16 +0.9 2,035,743 + Pf1N1B4_1783 0.16 +1.0 2,035,743 + Pf1N1B4_1783 0.16 -0.3 2,035,743 + Pf1N1B4_1783 0.16 -1.2 2,035,744 - Pf1N1B4_1783 0.16 +0.3 2,035,744 - Pf1N1B4_1783 0.16 -0.3 2,035,744 - Pf1N1B4_1783 0.16 -0.2 2,035,744 - Pf1N1B4_1783 0.16 -2.6 2,035,749 + Pf1N1B4_1783 0.16 -0.8 2,035,749 + Pf1N1B4_1783 0.16 -0.4 2,035,749 + Pf1N1B4_1783 0.16 +0.7 2,035,749 + Pf1N1B4_1783 0.16 +0.7 2,035,749 + Pf1N1B4_1783 0.16 -0.4 2,035,749 + Pf1N1B4_1783 0.16 -0.0 2,035,749 + Pf1N1B4_1783 0.16 +1.4 2,035,750 - Pf1N1B4_1783 0.16 -0.7 2,035,750 - Pf1N1B4_1783 0.16 -0.6 2,035,750 - Pf1N1B4_1783 0.16 -0.4 2,035,750 - Pf1N1B4_1783 0.16 +0.5 2,035,750 - Pf1N1B4_1783 0.16 +0.5 2,035,750 - Pf1N1B4_1783 0.16 -2.9 2,035,750 - Pf1N1B4_1783 0.16 -1.1 2,035,750 - Pf1N1B4_1783 0.16 -0.8 2,035,750 - Pf1N1B4_1783 0.16 +0.2 2,035,768 - Pf1N1B4_1783 0.18 -2.2 2,035,768 - Pf1N1B4_1783 0.18 +1.1 2,035,980 + Pf1N1B4_1783 0.39 +0.0 2,035,980 + Pf1N1B4_1783 0.39 +1.1 2,035,980 + Pf1N1B4_1783 0.39 -1.4 2,035,980 + Pf1N1B4_1783 0.39 +0.1 2,036,056 - Pf1N1B4_1783 0.47 -0.0 2,036,063 + Pf1N1B4_1783 0.48 +1.4 2,036,063 + Pf1N1B4_1783 0.48 +0.6 2,036,064 - Pf1N1B4_1783 0.48 -0.4 2,036,064 - Pf1N1B4_1783 0.48 -1.8 2,036,068 - Pf1N1B4_1783 0.48 -2.4 2,036,068 - Pf1N1B4_1783 0.48 +1.8 2,036,068 - Pf1N1B4_1783 0.48 -1.9 2,036,146 + Pf1N1B4_1783 0.56 -0.1 2,036,146 + Pf1N1B4_1783 0.56 +1.4 2,036,147 - Pf1N1B4_1783 0.56 +0.8 2,036,165 - Pf1N1B4_1783 0.58 +0.4 2,036,210 - Pf1N1B4_1783 0.63 -0.9 2,036,241 - Pf1N1B4_1783 0.66 +2.0 2,036,241 - Pf1N1B4_1783 0.66 +0.9 2,036,278 + Pf1N1B4_1783 0.69 +0.1 2,036,326 - Pf1N1B4_1783 0.74 +1.1 2,036,329 + Pf1N1B4_1783 0.75 -0.5 2,036,329 + Pf1N1B4_1783 0.75 -0.4 2,036,330 - Pf1N1B4_1783 0.75 +0.1 2,036,330 - Pf1N1B4_1783 0.75 +0.4 2,036,330 - Pf1N1B4_1783 0.75 -0.4 2,036,331 + Pf1N1B4_1783 0.75 +1.1 2,036,332 - Pf1N1B4_1783 0.75 +0.6 2,036,332 - Pf1N1B4_1783 0.75 -0.7 2,036,332 - Pf1N1B4_1783 0.75 +1.4 2,036,364 - Pf1N1B4_1783 0.78 +2.1 2,036,403 + Pf1N1B4_1783 0.82 -1.8 2,036,403 + Pf1N1B4_1783 0.82 -0.5 2,036,404 - Pf1N1B4_1783 0.82 -1.1 2,036,404 - Pf1N1B4_1783 0.82 +1.7 2,036,404 - Pf1N1B4_1783 0.82 -0.4
Or see this region's nucleotide sequence