Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1221 and Pf1N1B4_1222 are separated by 48 nucleotides Pf1N1B4_1222 and Pf1N1B4_1223 are separated by 69 nucleotides
Pf1N1B4_1221 - coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase, at 1,385,159 to 1,386,304
_1221
Pf1N1B4_1222 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 1,386,353 to 1,387,276
_1222
Pf1N1B4_1223 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 1,387,346 to 1,388,428
_1223
Position (kb)
1386
1387
1388 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1385.354 kb on + strand, within Pf1N1B4_1221 at 1385.354 kb on + strand, within Pf1N1B4_1221 at 1385.355 kb on - strand, within Pf1N1B4_1221 at 1385.355 kb on - strand, within Pf1N1B4_1221 at 1385.355 kb on - strand, within Pf1N1B4_1221 at 1385.355 kb on - strand, within Pf1N1B4_1221 at 1385.355 kb on - strand, within Pf1N1B4_1221 at 1385.441 kb on + strand, within Pf1N1B4_1221 at 1385.501 kb on - strand, within Pf1N1B4_1221 at 1385.533 kb on + strand, within Pf1N1B4_1221 at 1385.732 kb on + strand, within Pf1N1B4_1221 at 1385.732 kb on + strand, within Pf1N1B4_1221 at 1385.732 kb on + strand, within Pf1N1B4_1221 at 1385.733 kb on - strand, within Pf1N1B4_1221 at 1385.762 kb on - strand, within Pf1N1B4_1221 at 1385.872 kb on + strand, within Pf1N1B4_1221 at 1385.872 kb on + strand, within Pf1N1B4_1221 at 1385.873 kb on - strand, within Pf1N1B4_1221 at 1385.921 kb on + strand, within Pf1N1B4_1221 at 1385.921 kb on + strand, within Pf1N1B4_1221 at 1385.921 kb on + strand, within Pf1N1B4_1221 at 1385.921 kb on + strand, within Pf1N1B4_1221 at 1385.922 kb on - strand at 1386.138 kb on + strand, within Pf1N1B4_1221 at 1386.139 kb on - strand, within Pf1N1B4_1221 at 1386.139 kb on - strand, within Pf1N1B4_1221 at 1386.139 kb on - strand, within Pf1N1B4_1221 at 1386.426 kb on + strand at 1386.426 kb on + strand at 1386.427 kb on - strand at 1386.427 kb on - strand at 1386.501 kb on + strand, within Pf1N1B4_1222 at 1386.501 kb on + strand, within Pf1N1B4_1222 at 1386.501 kb on + strand, within Pf1N1B4_1222 at 1386.502 kb on - strand at 1386.502 kb on - strand, within Pf1N1B4_1222 at 1386.502 kb on - strand, within Pf1N1B4_1222 at 1386.657 kb on + strand, within Pf1N1B4_1222 at 1386.657 kb on + strand, within Pf1N1B4_1222 at 1386.741 kb on + strand, within Pf1N1B4_1222 at 1386.742 kb on - strand, within Pf1N1B4_1222 at 1386.742 kb on - strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.861 kb on + strand, within Pf1N1B4_1222 at 1386.862 kb on - strand, within Pf1N1B4_1222 at 1386.862 kb on - strand, within Pf1N1B4_1222 at 1386.862 kb on - strand, within Pf1N1B4_1222 at 1386.862 kb on - strand, within Pf1N1B4_1222 at 1386.864 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1387.162 kb on - strand, within Pf1N1B4_1222 at 1387.164 kb on + strand, within Pf1N1B4_1222 at 1387.164 kb on + strand, within Pf1N1B4_1222 at 1387.164 kb on + strand, within Pf1N1B4_1222 at 1387.164 kb on + strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.253 kb on + strand at 1387.266 kb on + strand at 1387.266 kb on + strand at 1387.266 kb on + strand at 1387.441 kb on - strand at 1387.530 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.722 kb on + strand, within Pf1N1B4_1223 at 1387.722 kb on + strand, within Pf1N1B4_1223 at 1387.722 kb on + strand, within Pf1N1B4_1223 at 1387.722 kb on + strand, within Pf1N1B4_1223 at 1387.722 kb on + strand, within Pf1N1B4_1223 at 1387.723 kb on - strand, within Pf1N1B4_1223 at 1387.723 kb on - strand, within Pf1N1B4_1223 at 1387.723 kb on - strand, within Pf1N1B4_1223 at 1387.723 kb on - strand, within Pf1N1B4_1223 at 1387.783 kb on - strand, within Pf1N1B4_1223 at 1387.783 kb on - strand, within Pf1N1B4_1223 at 1387.861 kb on - strand, within Pf1N1B4_1223 at 1387.881 kb on - strand, within Pf1N1B4_1223 at 1387.971 kb on + strand, within Pf1N1B4_1223 at 1387.972 kb on - strand, within Pf1N1B4_1223 at 1387.972 kb on - strand, within Pf1N1B4_1223 at 1388.019 kb on + strand, within Pf1N1B4_1223 at 1388.019 kb on + strand, within Pf1N1B4_1223 at 1388.115 kb on + strand, within Pf1N1B4_1223 at 1388.116 kb on - strand, within Pf1N1B4_1223 at 1388.116 kb on - strand, within Pf1N1B4_1223 at 1388.116 kb on - strand, within Pf1N1B4_1223 at 1388.128 kb on + strand, within Pf1N1B4_1223
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 1,385,354 + Pf1N1B4_1221 0.17 -0.1 1,385,354 + Pf1N1B4_1221 0.17 -0.5 1,385,355 - Pf1N1B4_1221 0.17 +1.4 1,385,355 - Pf1N1B4_1221 0.17 -2.1 1,385,355 - Pf1N1B4_1221 0.17 -0.4 1,385,355 - Pf1N1B4_1221 0.17 +0.9 1,385,355 - Pf1N1B4_1221 0.17 -1.7 1,385,441 + Pf1N1B4_1221 0.25 +1.0 1,385,501 - Pf1N1B4_1221 0.30 +0.4 1,385,533 + Pf1N1B4_1221 0.33 -0.0 1,385,732 + Pf1N1B4_1221 0.50 +0.4 1,385,732 + Pf1N1B4_1221 0.50 -0.8 1,385,732 + Pf1N1B4_1221 0.50 -0.8 1,385,733 - Pf1N1B4_1221 0.50 -1.8 1,385,762 - Pf1N1B4_1221 0.53 +0.4 1,385,872 + Pf1N1B4_1221 0.62 -0.8 1,385,872 + Pf1N1B4_1221 0.62 +0.0 1,385,873 - Pf1N1B4_1221 0.62 -0.9 1,385,921 + Pf1N1B4_1221 0.66 +0.6 1,385,921 + Pf1N1B4_1221 0.66 +0.3 1,385,921 + Pf1N1B4_1221 0.66 +0.2 1,385,921 + Pf1N1B4_1221 0.66 +2.1 1,385,922 - +2.4 1,386,138 + Pf1N1B4_1221 0.85 -1.0 1,386,139 - Pf1N1B4_1221 0.86 +0.8 1,386,139 - Pf1N1B4_1221 0.86 -2.4 1,386,139 - Pf1N1B4_1221 0.86 -0.2 1,386,426 + -2.2 1,386,426 + -0.9 1,386,427 - -1.6 1,386,427 - +1.4 1,386,501 + Pf1N1B4_1222 0.16 -0.1 1,386,501 + Pf1N1B4_1222 0.16 -1.0 1,386,501 + Pf1N1B4_1222 0.16 +0.4 1,386,502 - +0.1 1,386,502 - Pf1N1B4_1222 0.16 +1.0 1,386,502 - Pf1N1B4_1222 0.16 -0.8 1,386,657 + Pf1N1B4_1222 0.33 +0.4 1,386,657 + Pf1N1B4_1222 0.33 +0.7 1,386,741 + Pf1N1B4_1222 0.42 -0.4 1,386,742 - Pf1N1B4_1222 0.42 +0.6 1,386,742 - Pf1N1B4_1222 0.42 +0.8 1,386,747 + Pf1N1B4_1222 0.43 -0.2 1,386,747 + Pf1N1B4_1222 0.43 +0.4 1,386,747 + Pf1N1B4_1222 0.43 -0.6 1,386,747 + Pf1N1B4_1222 0.43 +0.4 1,386,747 + Pf1N1B4_1222 0.43 +0.4 1,386,747 + Pf1N1B4_1222 0.43 -1.4 1,386,747 + Pf1N1B4_1222 0.43 -1.3 1,386,748 - Pf1N1B4_1222 0.43 -0.9 1,386,748 - Pf1N1B4_1222 0.43 -1.6 1,386,748 - Pf1N1B4_1222 0.43 -0.9 1,386,748 - Pf1N1B4_1222 0.43 -0.3 1,386,748 - Pf1N1B4_1222 0.43 +0.5 1,386,748 - Pf1N1B4_1222 0.43 -1.4 1,386,748 - Pf1N1B4_1222 0.43 +2.0 1,386,861 + Pf1N1B4_1222 0.55 +0.5 1,386,862 - Pf1N1B4_1222 0.55 +0.2 1,386,862 - Pf1N1B4_1222 0.55 +1.8 1,386,862 - Pf1N1B4_1222 0.55 +0.2 1,386,862 - Pf1N1B4_1222 0.55 +0.4 1,386,864 + Pf1N1B4_1222 0.55 -0.4 1,386,962 + Pf1N1B4_1222 0.66 +2.2 1,386,962 + Pf1N1B4_1222 0.66 -1.8 1,386,962 + Pf1N1B4_1222 0.66 -0.1 1,386,962 + Pf1N1B4_1222 0.66 -1.1 1,386,962 + Pf1N1B4_1222 0.66 +0.1 1,386,962 + -0.2 1,386,962 + Pf1N1B4_1222 0.66 -0.5 1,386,962 + Pf1N1B4_1222 0.66 -1.1 1,386,962 + Pf1N1B4_1222 0.66 -1.6 1,386,962 + Pf1N1B4_1222 0.66 -1.4 1,386,962 + Pf1N1B4_1222 0.66 -1.8 1,386,962 + Pf1N1B4_1222 0.66 +0.4 1,386,962 + Pf1N1B4_1222 0.66 -2.3 1,386,962 + Pf1N1B4_1222 0.66 +0.4 1,386,963 - Pf1N1B4_1222 0.66 -1.3 1,386,963 - Pf1N1B4_1222 0.66 +1.0 1,386,963 - Pf1N1B4_1222 0.66 -0.2 1,386,963 - Pf1N1B4_1222 0.66 +0.6 1,386,963 - Pf1N1B4_1222 0.66 +0.6 1,386,963 - Pf1N1B4_1222 0.66 +1.9 1,386,963 - Pf1N1B4_1222 0.66 -0.6 1,386,963 - -1.9 1,386,963 - Pf1N1B4_1222 0.66 -0.7 1,386,963 - Pf1N1B4_1222 0.66 -1.3 1,386,963 - Pf1N1B4_1222 0.66 +1.4 1,386,963 - Pf1N1B4_1222 0.66 +1.4 1,386,963 - Pf1N1B4_1222 0.66 +0.5 1,386,963 - Pf1N1B4_1222 0.66 +0.1 1,386,963 - Pf1N1B4_1222 0.66 -0.9 1,386,963 - Pf1N1B4_1222 0.66 +0.6 1,386,963 - Pf1N1B4_1222 0.66 +2.0 1,386,963 - Pf1N1B4_1222 0.66 -0.9 1,386,963 - Pf1N1B4_1222 0.66 +1.8 1,387,162 - Pf1N1B4_1222 0.88 +1.7 1,387,164 + Pf1N1B4_1222 0.88 -0.0 1,387,164 + Pf1N1B4_1222 0.88 -2.2 1,387,164 + Pf1N1B4_1222 0.88 +0.5 1,387,164 + Pf1N1B4_1222 0.88 -0.4 1,387,165 - Pf1N1B4_1222 0.88 -0.5 1,387,165 - Pf1N1B4_1222 0.88 -0.7 1,387,165 - Pf1N1B4_1222 0.88 +0.1 1,387,165 - Pf1N1B4_1222 0.88 -1.4 1,387,165 - Pf1N1B4_1222 0.88 -0.2 1,387,165 - Pf1N1B4_1222 0.88 +0.1 1,387,165 - Pf1N1B4_1222 0.88 -0.2 1,387,253 + -2.5 1,387,266 + +1.4 1,387,266 + -1.9 1,387,266 + -0.2 1,387,441 - +1.8 1,387,530 + Pf1N1B4_1223 0.17 -0.4 1,387,686 + Pf1N1B4_1223 0.31 -0.6 1,387,686 + Pf1N1B4_1223 0.31 -0.3 1,387,686 + Pf1N1B4_1223 0.31 +0.9 1,387,686 + Pf1N1B4_1223 0.31 -0.5 1,387,686 + Pf1N1B4_1223 0.31 -0.5 1,387,686 + Pf1N1B4_1223 0.31 +3.4 1,387,686 + Pf1N1B4_1223 0.31 +1.0 1,387,686 + Pf1N1B4_1223 0.31 +1.6 1,387,687 - Pf1N1B4_1223 0.31 +0.5 1,387,687 - Pf1N1B4_1223 0.31 +0.4 1,387,687 - Pf1N1B4_1223 0.31 -1.7 1,387,687 - Pf1N1B4_1223 0.31 -0.8 1,387,687 - Pf1N1B4_1223 0.31 +2.0 1,387,687 - Pf1N1B4_1223 0.31 -0.2 1,387,687 - Pf1N1B4_1223 0.31 -0.9 1,387,722 + Pf1N1B4_1223 0.35 +1.4 1,387,722 + Pf1N1B4_1223 0.35 -0.2 1,387,722 + Pf1N1B4_1223 0.35 -1.4 1,387,722 + Pf1N1B4_1223 0.35 -0.6 1,387,722 + Pf1N1B4_1223 0.35 -0.7 1,387,723 - Pf1N1B4_1223 0.35 -0.5 1,387,723 - Pf1N1B4_1223 0.35 +0.4 1,387,723 - Pf1N1B4_1223 0.35 -0.8 1,387,723 - Pf1N1B4_1223 0.35 +0.1 1,387,783 - Pf1N1B4_1223 0.40 -0.0 1,387,783 - Pf1N1B4_1223 0.40 +2.4 1,387,861 - Pf1N1B4_1223 0.48 -1.3 1,387,881 - Pf1N1B4_1223 0.49 +0.8 1,387,971 + Pf1N1B4_1223 0.58 +1.4 1,387,972 - Pf1N1B4_1223 0.58 -0.2 1,387,972 - Pf1N1B4_1223 0.58 -2.5 1,388,019 + Pf1N1B4_1223 0.62 -0.9 1,388,019 + Pf1N1B4_1223 0.62 -0.4 1,388,115 + Pf1N1B4_1223 0.71 +1.0 1,388,116 - Pf1N1B4_1223 0.71 -0.4 1,388,116 - Pf1N1B4_1223 0.71 -2.3 1,388,116 - Pf1N1B4_1223 0.71 -0.6 1,388,128 + Pf1N1B4_1223 0.72 -1.1
Or see this region's nucleotide sequence