Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1190

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1188 and Pf1N1B4_1189 overlap by 1 nucleotidesPf1N1B4_1189 and Pf1N1B4_1190 overlap by 4 nucleotidesPf1N1B4_1190 and Pf1N1B4_1191 are separated by 129 nucleotidesPf1N1B4_1191 and Pf1N1B4_1192 are separated by 398 nucleotides Pf1N1B4_1188 - Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF, at 1,339,178 to 1,340,632 _1188 Pf1N1B4_1189 - Predicted L-lactate dehydrogenase, hypothetical protein subunit YkgG, at 1,340,632 to 1,341,303 _1189 Pf1N1B4_1190 - Predicted D-lactate dehydrogenase, Fe-S protein, FAD/FMN-containing, at 1,341,300 to 1,344,110 _1190 Pf1N1B4_1191 - hypothetical protein, at 1,344,240 to 1,344,521 _1191 Pf1N1B4_1192 - hypothetical protein, at 1,344,920 to 1,345,105 _1192 Position (kb) 1341 1342 1343 1344 1345Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1340.417 kb on + strand, within Pf1N1B4_1188at 1340.417 kb on + strand, within Pf1N1B4_1188at 1340.417 kb on + strand, within Pf1N1B4_1188at 1340.418 kb on - strand, within Pf1N1B4_1188at 1340.418 kb on - strand, within Pf1N1B4_1188at 1340.438 kb on - strand, within Pf1N1B4_1188at 1340.649 kb on + strandat 1340.649 kb on + strandat 1340.650 kb on - strandat 1340.650 kb on - strandat 1340.650 kb on - strandat 1340.731 kb on + strand, within Pf1N1B4_1189at 1340.737 kb on + strand, within Pf1N1B4_1189at 1340.737 kb on + strand, within Pf1N1B4_1189at 1340.737 kb on + strand, within Pf1N1B4_1189at 1340.737 kb on + strand, within Pf1N1B4_1189at 1340.737 kb on + strand, within Pf1N1B4_1189at 1340.738 kb on - strand, within Pf1N1B4_1189at 1340.738 kb on - strand, within Pf1N1B4_1189at 1340.738 kb on - strand, within Pf1N1B4_1189at 1340.738 kb on - strand, within Pf1N1B4_1189at 1340.738 kb on - strand, within Pf1N1B4_1189at 1340.738 kb on - strand, within Pf1N1B4_1189at 1340.738 kb on - strand, within Pf1N1B4_1189at 1340.953 kb on + strand, within Pf1N1B4_1189at 1340.953 kb on + strandat 1340.953 kb on + strand, within Pf1N1B4_1189at 1340.954 kb on - strand, within Pf1N1B4_1189at 1341.046 kb on - strand, within Pf1N1B4_1189at 1341.242 kb on + strandat 1341.242 kb on + strandat 1341.242 kb on + strandat 1341.242 kb on + strandat 1341.774 kb on + strand, within Pf1N1B4_1190at 1341.774 kb on + strand, within Pf1N1B4_1190at 1341.774 kb on + strand, within Pf1N1B4_1190at 1341.774 kb on + strand, within Pf1N1B4_1190at 1341.774 kb on + strand, within Pf1N1B4_1190at 1341.775 kb on - strand, within Pf1N1B4_1190at 1341.775 kb on - strand, within Pf1N1B4_1190at 1341.775 kb on - strand, within Pf1N1B4_1190at 1341.951 kb on + strand, within Pf1N1B4_1190at 1341.952 kb on - strand, within Pf1N1B4_1190at 1341.952 kb on - strand, within Pf1N1B4_1190at 1341.952 kb on - strand, within Pf1N1B4_1190at 1341.952 kb on - strand, within Pf1N1B4_1190at 1341.952 kb on - strand, within Pf1N1B4_1190at 1341.966 kb on - strand, within Pf1N1B4_1190at 1342.017 kb on - strand, within Pf1N1B4_1190at 1342.062 kb on - strand, within Pf1N1B4_1190at 1342.077 kb on + strand, within Pf1N1B4_1190at 1342.077 kb on + strand, within Pf1N1B4_1190at 1342.078 kb on - strand, within Pf1N1B4_1190at 1342.078 kb on - strand, within Pf1N1B4_1190at 1342.078 kb on - strand, within Pf1N1B4_1190at 1342.078 kb on - strand, within Pf1N1B4_1190at 1342.135 kb on + strandat 1342.135 kb on + strand, within Pf1N1B4_1190at 1342.136 kb on - strand, within Pf1N1B4_1190at 1342.136 kb on - strand, within Pf1N1B4_1190at 1342.136 kb on - strand, within Pf1N1B4_1190at 1342.136 kb on - strand, within Pf1N1B4_1190at 1342.252 kb on + strand, within Pf1N1B4_1190at 1342.253 kb on - strand, within Pf1N1B4_1190at 1342.779 kb on + strand, within Pf1N1B4_1190at 1342.817 kb on + strand, within Pf1N1B4_1190at 1342.914 kb on - strand, within Pf1N1B4_1190at 1342.914 kb on - strand, within Pf1N1B4_1190at 1343.004 kb on - strand, within Pf1N1B4_1190at 1343.055 kb on + strand, within Pf1N1B4_1190at 1343.075 kb on + strand, within Pf1N1B4_1190at 1343.076 kb on - strand, within Pf1N1B4_1190at 1343.116 kb on + strand, within Pf1N1B4_1190at 1343.231 kb on + strand, within Pf1N1B4_1190at 1343.232 kb on - strand, within Pf1N1B4_1190at 1343.412 kb on + strand, within Pf1N1B4_1190at 1343.412 kb on + strand, within Pf1N1B4_1190at 1343.412 kb on + strand, within Pf1N1B4_1190at 1343.412 kb on + strand, within Pf1N1B4_1190at 1343.413 kb on - strand, within Pf1N1B4_1190at 1343.413 kb on - strand, within Pf1N1B4_1190at 1343.459 kb on + strand, within Pf1N1B4_1190at 1343.460 kb on - strand, within Pf1N1B4_1190at 1343.661 kb on + strand, within Pf1N1B4_1190at 1343.661 kb on + strand, within Pf1N1B4_1190at 1343.661 kb on + strand, within Pf1N1B4_1190at 1343.661 kb on + strand, within Pf1N1B4_1190at 1343.661 kb on + strand, within Pf1N1B4_1190at 1343.662 kb on - strand, within Pf1N1B4_1190at 1343.662 kb on - strand, within Pf1N1B4_1190at 1343.662 kb on - strand, within Pf1N1B4_1190at 1343.727 kb on + strand, within Pf1N1B4_1190at 1343.727 kb on + strand, within Pf1N1B4_1190at 1343.727 kb on + strand, within Pf1N1B4_1190at 1343.727 kb on + strand, within Pf1N1B4_1190at 1343.727 kb on + strand, within Pf1N1B4_1190at 1343.728 kb on - strand, within Pf1N1B4_1190at 1343.728 kb on - strand, within Pf1N1B4_1190at 1343.728 kb on - strand, within Pf1N1B4_1190at 1343.728 kb on - strand, within Pf1N1B4_1190at 1343.873 kb on + strandat 1343.874 kb on - strandat 1344.058 kb on - strandat 1344.072 kb on + strandat 1344.072 kb on + strandat 1344.072 kb on + strandat 1344.073 kb on - strandat 1344.073 kb on - strandat 1344.073 kb on - strandat 1344.118 kb on + strandat 1344.119 kb on - strandat 1344.254 kb on - strandat 1344.259 kb on + strandat 1344.259 kb on + strandat 1344.260 kb on - strandat 1344.420 kb on + strand, within Pf1N1B4_1191at 1344.420 kb on + strand, within Pf1N1B4_1191at 1344.421 kb on - strand, within Pf1N1B4_1191at 1344.421 kb on - strand, within Pf1N1B4_1191at 1344.490 kb on + strandat 1344.490 kb on + strand, within Pf1N1B4_1191at 1344.491 kb on - strand, within Pf1N1B4_1191at 1344.513 kb on - strandat 1344.523 kb on - strandat 1344.565 kb on + strandat 1344.565 kb on + strandat 1344.566 kb on - strandat 1344.566 kb on - strandat 1344.566 kb on - strandat 1344.572 kb on + strandat 1344.573 kb on - strandat 1344.645 kb on + strandat 1344.646 kb on - strandat 1344.664 kb on + strandat 1344.665 kb on - strandat 1344.665 kb on - strandat 1344.826 kb on - strandat 1344.890 kb on + strandat 1344.891 kb on - strandat 1344.919 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
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1,340,417 + Pf1N1B4_1188 0.85 -0.7
1,340,417 + Pf1N1B4_1188 0.85 +1.4
1,340,417 + Pf1N1B4_1188 0.85 +0.6
1,340,418 - Pf1N1B4_1188 0.85 +0.5
1,340,418 - Pf1N1B4_1188 0.85 -0.1
1,340,438 - Pf1N1B4_1188 0.87 -0.3
1,340,649 + +0.5
1,340,649 + -1.4
1,340,650 - +2.4
1,340,650 - +1.7
1,340,650 - -0.9
1,340,731 + Pf1N1B4_1189 0.15 +0.4
1,340,737 + Pf1N1B4_1189 0.16 +0.7
1,340,737 + Pf1N1B4_1189 0.16 -0.9
1,340,737 + Pf1N1B4_1189 0.16 -0.2
1,340,737 + Pf1N1B4_1189 0.16 -0.0
1,340,737 + Pf1N1B4_1189 0.16 +2.7
1,340,738 - Pf1N1B4_1189 0.16 +1.4
1,340,738 - Pf1N1B4_1189 0.16 +1.8
1,340,738 - Pf1N1B4_1189 0.16 +0.4
1,340,738 - Pf1N1B4_1189 0.16 -0.2
1,340,738 - Pf1N1B4_1189 0.16 +0.7
1,340,738 - Pf1N1B4_1189 0.16 -0.4
1,340,738 - Pf1N1B4_1189 0.16 +2.2
1,340,953 + Pf1N1B4_1189 0.48 -1.6
1,340,953 + +3.0
1,340,953 + Pf1N1B4_1189 0.48 +0.1
1,340,954 - Pf1N1B4_1189 0.48 +0.3
1,341,046 - Pf1N1B4_1189 0.62 +0.0
1,341,242 + -1.6
1,341,242 + -0.6
1,341,242 + +1.9
1,341,242 + -0.7
1,341,774 + Pf1N1B4_1190 0.17 +2.0
1,341,774 + Pf1N1B4_1190 0.17 +0.4
1,341,774 + Pf1N1B4_1190 0.17 +0.4
1,341,774 + Pf1N1B4_1190 0.17 +1.0
1,341,774 + Pf1N1B4_1190 0.17 +0.7
1,341,775 - Pf1N1B4_1190 0.17 +0.4
1,341,775 - Pf1N1B4_1190 0.17 +2.2
1,341,775 - Pf1N1B4_1190 0.17 +0.5
1,341,951 + Pf1N1B4_1190 0.23 +0.5
1,341,952 - Pf1N1B4_1190 0.23 +0.7
1,341,952 - Pf1N1B4_1190 0.23 -0.5
1,341,952 - Pf1N1B4_1190 0.23 +0.9
1,341,952 - Pf1N1B4_1190 0.23 +1.1
1,341,952 - Pf1N1B4_1190 0.23 -0.1
1,341,966 - Pf1N1B4_1190 0.24 +0.9
1,342,017 - Pf1N1B4_1190 0.26 +1.0
1,342,062 - Pf1N1B4_1190 0.27 -1.2
1,342,077 + Pf1N1B4_1190 0.28 +0.9
1,342,077 + Pf1N1B4_1190 0.28 +0.6
1,342,078 - Pf1N1B4_1190 0.28 +0.8
1,342,078 - Pf1N1B4_1190 0.28 -0.1
1,342,078 - Pf1N1B4_1190 0.28 -0.0
1,342,078 - Pf1N1B4_1190 0.28 +1.8
1,342,135 + -1.4
1,342,135 + Pf1N1B4_1190 0.30 -0.2
1,342,136 - Pf1N1B4_1190 0.30 +1.0
1,342,136 - Pf1N1B4_1190 0.30 -0.2
1,342,136 - Pf1N1B4_1190 0.30 -0.7
1,342,136 - Pf1N1B4_1190 0.30 -1.9
1,342,252 + Pf1N1B4_1190 0.34 -1.4
1,342,253 - Pf1N1B4_1190 0.34 -2.2
1,342,779 + Pf1N1B4_1190 0.53 +0.2
1,342,817 + Pf1N1B4_1190 0.54 +0.8
1,342,914 - Pf1N1B4_1190 0.57 +1.1
1,342,914 - Pf1N1B4_1190 0.57 +1.4
1,343,004 - Pf1N1B4_1190 0.61 +0.9
1,343,055 + Pf1N1B4_1190 0.62 +1.4
1,343,075 + Pf1N1B4_1190 0.63 -0.6
1,343,076 - Pf1N1B4_1190 0.63 +0.4
1,343,116 + Pf1N1B4_1190 0.65 -0.9
1,343,231 + Pf1N1B4_1190 0.69 +0.0
1,343,232 - Pf1N1B4_1190 0.69 -0.3
1,343,412 + Pf1N1B4_1190 0.75 -0.7
1,343,412 + Pf1N1B4_1190 0.75 -1.6
1,343,412 + Pf1N1B4_1190 0.75 +1.9
1,343,412 + Pf1N1B4_1190 0.75 +1.7
1,343,413 - Pf1N1B4_1190 0.75 -0.8
1,343,413 - Pf1N1B4_1190 0.75 -0.9
1,343,459 + Pf1N1B4_1190 0.77 +2.0
1,343,460 - Pf1N1B4_1190 0.77 +0.5
1,343,661 + Pf1N1B4_1190 0.84 -0.7
1,343,661 + Pf1N1B4_1190 0.84 -0.5
1,343,661 + Pf1N1B4_1190 0.84 +2.0
1,343,661 + Pf1N1B4_1190 0.84 +0.2
1,343,661 + Pf1N1B4_1190 0.84 +1.6
1,343,662 - Pf1N1B4_1190 0.84 +0.4
1,343,662 - Pf1N1B4_1190 0.84 -2.0
1,343,662 - Pf1N1B4_1190 0.84 +1.1
1,343,727 + Pf1N1B4_1190 0.86 +0.4
1,343,727 + Pf1N1B4_1190 0.86 +1.0
1,343,727 + Pf1N1B4_1190 0.86 +0.9
1,343,727 + Pf1N1B4_1190 0.86 +1.1
1,343,727 + Pf1N1B4_1190 0.86 +2.0
1,343,728 - Pf1N1B4_1190 0.86 +0.9
1,343,728 - Pf1N1B4_1190 0.86 -0.4
1,343,728 - Pf1N1B4_1190 0.86 +1.2
1,343,728 - Pf1N1B4_1190 0.86 +0.6
1,343,873 + +0.8
1,343,874 - -0.6
1,344,058 - +0.4
1,344,072 + -1.9
1,344,072 + +0.8
1,344,072 + +0.3
1,344,073 - -0.1
1,344,073 - +0.7
1,344,073 - +1.0
1,344,118 + -0.8
1,344,119 - -0.9
1,344,254 - +0.1
1,344,259 + -0.6
1,344,259 + -0.4
1,344,260 - -0.2
1,344,420 + Pf1N1B4_1191 0.64 +0.4
1,344,420 + Pf1N1B4_1191 0.64 -2.4
1,344,421 - Pf1N1B4_1191 0.64 +0.6
1,344,421 - Pf1N1B4_1191 0.64 -0.2
1,344,490 + +0.2
1,344,490 + Pf1N1B4_1191 0.89 -0.2
1,344,491 - Pf1N1B4_1191 0.89 -0.1
1,344,513 - -1.2
1,344,523 - -0.1
1,344,565 + -1.1
1,344,565 + +0.1
1,344,566 - +0.1
1,344,566 - -0.0
1,344,566 - -0.9
1,344,572 + -0.2
1,344,573 - +0.1
1,344,645 + +0.1
1,344,646 - -0.2
1,344,664 + +0.4
1,344,665 - +0.3
1,344,665 - -0.4
1,344,826 - -0.0
1,344,890 + +0.6
1,344,891 - +1.4
1,344,919 - +1.1

Or see this region's nucleotide sequence