Experiment: Varel_Bryant_medium_Glucose with Thioridazine 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1604 and BT1605 overlap by 17 nucleotides BT1605 and BT1606 are separated by 20 nucleotides
BT1604: BT1604 - conserved hypothetical protein (NCBI ptt file), at 1,975,123 to 1,976,001
BT1604
BT1605: BT1605 - cytochrome c biogenesis protein (NCBI ptt file), at 1,975,985 to 1,976,770
BT1605
BT1606: BT1606 - cytochrome C peroxidase (NCBI ptt file), at 1,976,791 to 1,978,173
BT1606
Position (kb)
1975
1976
1977 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1974.988 kb on + strand at 1975.021 kb on - strand at 1975.088 kb on + strand at 1975.103 kb on - strand at 1975.127 kb on - strand at 1975.127 kb on - strand at 1975.127 kb on - strand at 1975.127 kb on - strand at 1975.133 kb on + strand at 1975.133 kb on + strand at 1975.133 kb on + strand at 1975.135 kb on + strand at 1975.140 kb on - strand at 1975.154 kb on + strand at 1975.158 kb on - strand at 1975.267 kb on - strand, within BT1604 at 1975.278 kb on + strand, within BT1604 at 1975.380 kb on - strand, within BT1604 at 1975.380 kb on - strand, within BT1604 at 1975.455 kb on - strand, within BT1604 at 1975.465 kb on - strand, within BT1604 at 1975.465 kb on - strand, within BT1604 at 1975.473 kb on - strand, within BT1604 at 1975.473 kb on - strand, within BT1604 at 1975.475 kb on + strand, within BT1604 at 1975.557 kb on + strand, within BT1604 at 1975.596 kb on + strand, within BT1604 at 1975.622 kb on + strand, within BT1604 at 1975.631 kb on - strand, within BT1604 at 1975.679 kb on - strand, within BT1604 at 1975.696 kb on - strand, within BT1604 at 1975.834 kb on + strand, within BT1604 at 1975.912 kb on + strand, within BT1604 at 1975.913 kb on - strand, within BT1604 at 1976.010 kb on + strand at 1976.023 kb on - strand at 1976.034 kb on + strand at 1976.116 kb on + strand, within BT1605 at 1976.117 kb on - strand, within BT1605 at 1976.119 kb on - strand, within BT1605 at 1976.170 kb on - strand, within BT1605 at 1976.227 kb on - strand, within BT1605 at 1976.234 kb on + strand, within BT1605 at 1976.234 kb on + strand, within BT1605 at 1976.237 kb on - strand, within BT1605 at 1976.250 kb on - strand, within BT1605 at 1976.303 kb on - strand, within BT1605 at 1976.382 kb on + strand, within BT1605 at 1976.397 kb on - strand, within BT1605 at 1976.468 kb on - strand, within BT1605 at 1976.720 kb on + strand at 1976.721 kb on - strand at 1976.765 kb on - strand at 1976.765 kb on - strand at 1976.769 kb on - strand at 1976.769 kb on - strand at 1976.901 kb on - strand at 1976.901 kb on - strand at 1976.916 kb on + strand at 1976.917 kb on - strand at 1977.099 kb on + strand, within BT1606 at 1977.100 kb on - strand, within BT1606 at 1977.104 kb on + strand, within BT1606 at 1977.145 kb on + strand, within BT1606 at 1977.148 kb on + strand, within BT1606 at 1977.156 kb on - strand, within BT1606 at 1977.195 kb on - strand, within BT1606 at 1977.233 kb on - strand, within BT1606 at 1977.283 kb on - strand, within BT1606 at 1977.309 kb on - strand, within BT1606 at 1977.337 kb on + strand, within BT1606 at 1977.475 kb on + strand, within BT1606 at 1977.476 kb on - strand, within BT1606 at 1977.489 kb on + strand, within BT1606 at 1977.629 kb on + strand, within BT1606 at 1977.645 kb on - strand, within BT1606 at 1977.645 kb on - strand, within BT1606 at 1977.645 kb on - strand, within BT1606 at 1977.717 kb on + strand, within BT1606
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Thioridazine 0.025 mM remove 1,974,988 + -1.6 1,975,021 - -3.3 1,975,088 + -2.3 1,975,103 - -1.0 1,975,127 - +0.7 1,975,127 - -1.0 1,975,127 - -0.4 1,975,127 - -0.0 1,975,133 + -2.8 1,975,133 + -0.0 1,975,133 + +0.3 1,975,135 + -0.0 1,975,140 - -0.0 1,975,154 + +3.1 1,975,158 - +0.4 1,975,267 - BT1604 0.16 -2.3 1,975,278 + BT1604 0.18 -0.7 1,975,380 - BT1604 0.29 -1.6 1,975,380 - BT1604 0.29 -1.3 1,975,455 - BT1604 0.38 -2.8 1,975,465 - BT1604 0.39 -2.2 1,975,465 - BT1604 0.39 +0.1 1,975,473 - BT1604 0.40 -1.0 1,975,473 - BT1604 0.40 -0.5 1,975,475 + BT1604 0.40 -2.8 1,975,557 + BT1604 0.49 -1.8 1,975,596 + BT1604 0.54 -2.0 1,975,622 + BT1604 0.57 +0.2 1,975,631 - BT1604 0.58 -0.0 1,975,679 - BT1604 0.63 -1.6 1,975,696 - BT1604 0.65 -0.0 1,975,834 + BT1604 0.81 +2.8 1,975,912 + BT1604 0.90 -0.0 1,975,913 - BT1604 0.90 -2.0 1,976,010 + -1.0 1,976,023 - -1.3 1,976,034 + +1.6 1,976,116 + BT1605 0.17 +1.6 1,976,117 - BT1605 0.17 -1.0 1,976,119 - BT1605 0.17 +3.0 1,976,170 - BT1605 0.24 -0.0 1,976,227 - BT1605 0.31 +2.6 1,976,234 + BT1605 0.32 +3.3 1,976,234 + BT1605 0.32 -2.0 1,976,237 - BT1605 0.32 +2.1 1,976,250 - BT1605 0.34 -3.8 1,976,303 - BT1605 0.40 -1.6 1,976,382 + BT1605 0.51 -0.0 1,976,397 - BT1605 0.52 +1.4 1,976,468 - BT1605 0.61 +1.1 1,976,720 + -0.0 1,976,721 - +1.6 1,976,765 - -2.0 1,976,765 - -1.0 1,976,769 - -0.4 1,976,769 - -2.0 1,976,901 - -0.0 1,976,901 - -2.0 1,976,916 + -1.0 1,976,917 - -2.2 1,977,099 + BT1606 0.22 -1.6 1,977,100 - BT1606 0.22 -2.8 1,977,104 + BT1606 0.23 -3.8 1,977,145 + BT1606 0.26 -0.3 1,977,148 + BT1606 0.26 +0.4 1,977,156 - BT1606 0.26 -2.8 1,977,195 - BT1606 0.29 -3.2 1,977,233 - BT1606 0.32 -2.3 1,977,283 - BT1606 0.36 -0.0 1,977,309 - BT1606 0.37 +0.3 1,977,337 + BT1606 0.39 +1.2 1,977,475 + BT1606 0.49 -1.3 1,977,476 - BT1606 0.50 -1.6 1,977,489 + BT1606 0.50 +2.3 1,977,629 + BT1606 0.61 -0.8 1,977,645 - BT1606 0.62 -1.6 1,977,645 - BT1606 0.62 -1.0 1,977,645 - BT1606 0.62 -0.4 1,977,717 + BT1606 0.67 -1.6
Or see this region's nucleotide sequence