Experiment: Varel_Bryant_medium_Glucose with Thioridazine 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0456 and BT0457 are separated by 11 nucleotides BT0457 and BT0458 are separated by 77 nucleotides
BT0456: BT0456 - beta-hexosaminidase precursor (NCBI ptt file), at 559,086 to 561,098
BT0456
BT0457: BT0457 - sialic acid-specific 9-O-acetylesterase (NCBI ptt file), at 561,110 to 563,188
BT0457
BT0458: BT0458 - beta-mannosidase precursor (NCBI ptt file), at 563,266 to 565,860
BT0458
Position (kb) 561
562
563
564 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 560.153 kb on - strand, within BT0456 at 560.153 kb on - strand, within BT0456 at 560.169 kb on - strand, within BT0456 at 560.261 kb on + strand, within BT0456 at 560.338 kb on - strand, within BT0456 at 560.423 kb on - strand, within BT0456 at 560.464 kb on - strand, within BT0456 at 560.566 kb on - strand, within BT0456 at 560.566 kb on - strand, within BT0456 at 560.567 kb on + strand, within BT0456 at 560.568 kb on - strand, within BT0456 at 560.571 kb on + strand, within BT0456 at 560.616 kb on - strand, within BT0456 at 560.633 kb on - strand, within BT0456 at 560.689 kb on - strand, within BT0456 at 560.689 kb on - strand, within BT0456 at 560.695 kb on - strand, within BT0456 at 560.862 kb on - strand, within BT0456 at 560.943 kb on - strand at 561.002 kb on - strand at 561.108 kb on - strand at 561.126 kb on + strand at 561.152 kb on - strand at 561.215 kb on + strand at 561.242 kb on - strand at 561.347 kb on + strand, within BT0457 at 561.348 kb on - strand, within BT0457 at 561.394 kb on + strand at 561.424 kb on + strand, within BT0457 at 561.425 kb on - strand at 561.539 kb on - strand, within BT0457 at 561.539 kb on - strand, within BT0457 at 561.539 kb on - strand, within BT0457 at 561.539 kb on - strand, within BT0457 at 561.700 kb on - strand, within BT0457 at 561.724 kb on + strand, within BT0457 at 561.744 kb on - strand, within BT0457 at 561.793 kb on + strand, within BT0457 at 561.871 kb on - strand, within BT0457 at 561.961 kb on + strand, within BT0457 at 562.105 kb on - strand, within BT0457 at 562.205 kb on + strand, within BT0457 at 562.206 kb on - strand, within BT0457 at 562.497 kb on - strand, within BT0457 at 562.512 kb on + strand, within BT0457 at 562.677 kb on - strand, within BT0457 at 562.681 kb on - strand, within BT0457 at 562.810 kb on + strand, within BT0457 at 562.810 kb on + strand, within BT0457 at 562.810 kb on + strand, within BT0457 at 562.816 kb on + strand, within BT0457 at 562.816 kb on + strand, within BT0457 at 562.935 kb on + strand, within BT0457 at 563.063 kb on + strand at 563.064 kb on - strand at 563.266 kb on - strand at 563.325 kb on + strand at 563.329 kb on - strand at 563.335 kb on + strand at 563.347 kb on - strand at 563.370 kb on - strand at 563.536 kb on + strand, within BT0458 at 563.539 kb on - strand, within BT0458 at 563.539 kb on - strand, within BT0458 at 563.550 kb on + strand, within BT0458 at 563.551 kb on - strand, within BT0458 at 563.615 kb on - strand, within BT0458 at 563.677 kb on - strand, within BT0458 at 563.721 kb on - strand, within BT0458 at 563.848 kb on - strand, within BT0458 at 563.848 kb on - strand, within BT0458 at 563.925 kb on - strand, within BT0458 at 563.925 kb on - strand, within BT0458 at 563.925 kb on - strand, within BT0458 at 563.925 kb on - strand, within BT0458 at 563.928 kb on + strand, within BT0458 at 564.051 kb on - strand, within BT0458 at 564.066 kb on + strand, within BT0458 at 564.110 kb on + strand, within BT0458
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Thioridazine 0.025 mM remove 560,153 - BT0456 0.53 -0.1 560,153 - BT0456 0.53 -0.7 560,169 - BT0456 0.54 -0.1 560,261 + BT0456 0.58 -3.3 560,338 - BT0456 0.62 -1.9 560,423 - BT0456 0.66 -1.1 560,464 - BT0456 0.68 -2.4 560,566 - BT0456 0.74 -0.1 560,566 - BT0456 0.74 -1.7 560,567 + BT0456 0.74 -1.4 560,568 - BT0456 0.74 -2.1 560,571 + BT0456 0.74 -0.1 560,616 - BT0456 0.76 -1.8 560,633 - BT0456 0.77 -1.1 560,689 - BT0456 0.80 -0.9 560,689 - BT0456 0.80 +0.5 560,695 - BT0456 0.80 -0.1 560,862 - BT0456 0.88 -2.1 560,943 - +0.7 561,002 - -0.1 561,108 - -1.7 561,126 + +2.2 561,152 - -1.1 561,215 + -1.1 561,242 - -1.7 561,347 + BT0457 0.11 -1.7 561,348 - BT0457 0.11 -3.3 561,394 + +1.2 561,424 + BT0457 0.15 -3.7 561,425 - -0.1 561,539 - BT0457 0.21 -0.1 561,539 - BT0457 0.21 -0.1 561,539 - BT0457 0.21 -1.8 561,539 - BT0457 0.21 -0.1 561,700 - BT0457 0.28 -2.9 561,724 + BT0457 0.30 -2.8 561,744 - BT0457 0.30 -0.1 561,793 + BT0457 0.33 -1.7 561,871 - BT0457 0.37 -1.7 561,961 + BT0457 0.41 -1.1 562,105 - BT0457 0.48 -1.7 562,205 + BT0457 0.53 -0.4 562,206 - BT0457 0.53 +2.8 562,497 - BT0457 0.67 -2.3 562,512 + BT0457 0.67 +0.1 562,677 - BT0457 0.75 -1.7 562,681 - BT0457 0.76 -2.0 562,810 + BT0457 0.82 +1.7 562,810 + BT0457 0.82 +1.9 562,810 + BT0457 0.82 -1.7 562,816 + BT0457 0.82 -1.4 562,816 + BT0457 0.82 -0.1 562,935 + BT0457 0.88 -2.1 563,063 + +0.1 563,064 - -3.1 563,266 - -1.1 563,325 + -1.9 563,329 - -1.1 563,335 + -1.1 563,347 - -0.1 563,370 - -1.1 563,536 + BT0458 0.10 -1.1 563,539 - BT0458 0.11 -0.6 563,539 - BT0458 0.11 -0.1 563,550 + BT0458 0.11 -0.1 563,551 - BT0458 0.11 -1.5 563,615 - BT0458 0.13 +1.9 563,677 - BT0458 0.16 -0.7 563,721 - BT0458 0.18 -1.7 563,848 - BT0458 0.22 -1.1 563,848 - BT0458 0.22 -0.5 563,925 - BT0458 0.25 +2.2 563,925 - BT0458 0.25 -0.1 563,925 - BT0458 0.25 +0.4 563,925 - BT0458 0.25 +1.4 563,928 + BT0458 0.26 -1.0 564,051 - BT0458 0.30 +1.0 564,066 + BT0458 0.31 -1.7 564,110 + BT0458 0.33 -2.9
Or see this region's nucleotide sequence