Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT1288

Experiment: Varel_Bryant_medium_Glucose with Ramoplanin 0.0015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT1287 and BT1288 are separated by 200 nucleotidesBT1288 and BT1289 are separated by 23 nucleotidesBT1289 and BT1290 overlap by 7 nucleotides BT1287: BT1287 - hypothetical protein (NCBI ptt file), at 1,603,811 to 1,604,449 BT1287 BT1288: BT1288 - spermidine/putrescine ABC transporter (NCBI ptt file), at 1,604,650 to 1,605,999 BT1288 BT1289: BT1289 - putrescine transport system permease protein potI (NCBI ptt file), at 1,606,023 to 1,606,820 BT1289 BT1290: BT1290 - putrescine transport system permease protein potH (NCBI ptt file), at 1,606,814 to 1,607,590 BT1290 Position (kb) 1604 1605 1606Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1603.735 kb on + strandat 1603.736 kb on - strandat 1603.736 kb on - strandat 1603.741 kb on - strandat 1603.745 kb on - strandat 1603.749 kb on - strandat 1603.768 kb on + strandat 1603.768 kb on + strandat 1603.874 kb on - strandat 1603.891 kb on + strand, within BT1287at 1603.917 kb on + strand, within BT1287at 1603.936 kb on - strand, within BT1287at 1603.946 kb on + strand, within BT1287at 1604.042 kb on - strand, within BT1287at 1604.050 kb on - strand, within BT1287at 1604.053 kb on - strand, within BT1287at 1604.147 kb on + strand, within BT1287at 1604.330 kb on + strand, within BT1287at 1604.330 kb on + strand, within BT1287at 1604.331 kb on - strand, within BT1287at 1604.440 kb on + strandat 1604.460 kb on + strandat 1604.566 kb on - strandat 1604.566 kb on - strandat 1604.606 kb on - strandat 1604.616 kb on + strandat 1604.631 kb on - strandat 1604.714 kb on + strandat 1604.758 kb on + strandat 1604.785 kb on - strand, within BT1288at 1604.898 kb on - strand, within BT1288at 1604.977 kb on - strand, within BT1288at 1605.006 kb on + strand, within BT1288at 1605.111 kb on - strand, within BT1288at 1605.134 kb on + strand, within BT1288at 1605.141 kb on + strand, within BT1288at 1605.235 kb on - strand, within BT1288at 1605.235 kb on - strand, within BT1288at 1605.235 kb on - strand, within BT1288at 1605.339 kb on + strand, within BT1288at 1605.418 kb on - strand, within BT1288at 1605.418 kb on - strand, within BT1288at 1605.525 kb on - strand, within BT1288at 1605.525 kb on - strand, within BT1288at 1605.537 kb on - strand, within BT1288at 1605.544 kb on - strand, within BT1288at 1605.561 kb on + strand, within BT1288at 1605.562 kb on - strand, within BT1288at 1605.609 kb on + strand, within BT1288at 1605.613 kb on - strand, within BT1288at 1605.650 kb on + strand, within BT1288at 1605.651 kb on - strand, within BT1288at 1605.722 kb on + strand, within BT1288at 1605.722 kb on + strand, within BT1288at 1605.723 kb on - strand, within BT1288at 1605.806 kb on + strand, within BT1288at 1605.820 kb on - strand, within BT1288at 1605.879 kb on + strandat 1605.882 kb on - strandat 1605.895 kb on - strandat 1605.896 kb on + strandat 1605.897 kb on - strandat 1605.897 kb on - strandat 1605.936 kb on - strandat 1605.936 kb on - strandat 1606.016 kb on + strandat 1606.017 kb on - strandat 1606.068 kb on + strandat 1606.159 kb on + strand, within BT1289at 1606.159 kb on + strand, within BT1289at 1606.159 kb on + strand, within BT1289at 1606.159 kb on + strand, within BT1289at 1606.160 kb on - strand, within BT1289at 1606.196 kb on + strand, within BT1289at 1606.196 kb on + strand, within BT1289at 1606.304 kb on + strand, within BT1289at 1606.378 kb on - strand, within BT1289at 1606.430 kb on - strand, within BT1289at 1606.459 kb on - strand, within BT1289at 1606.487 kb on + strand, within BT1289at 1606.508 kb on + strand, within BT1289at 1606.509 kb on - strand, within BT1289at 1606.590 kb on - strand, within BT1289at 1606.625 kb on + strand, within BT1289at 1606.739 kb on - strand, within BT1289at 1606.767 kb on + strandat 1606.780 kb on - strandat 1606.816 kb on - strandat 1606.821 kb on - strandat 1606.841 kb on - strandat 1606.927 kb on + strand, within BT1290at 1606.927 kb on + strand, within BT1290at 1606.957 kb on + strand, within BT1290

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Per-strain Table

Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Ramoplanin 0.0015625 mM
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1,603,735 + +1.1
1,603,736 - +0.1
1,603,736 - +0.7
1,603,741 - +0.1
1,603,745 - +0.1
1,603,749 - -1.4
1,603,768 + -1.9
1,603,768 + +1.1
1,603,874 - +1.1
1,603,891 + BT1287 0.13 +0.6
1,603,917 + BT1287 0.17 +1.6
1,603,936 - BT1287 0.20 +0.1
1,603,946 + BT1287 0.21 -0.9
1,604,042 - BT1287 0.36 -0.9
1,604,050 - BT1287 0.37 -0.9
1,604,053 - BT1287 0.38 -0.3
1,604,147 + BT1287 0.53 +0.4
1,604,330 + BT1287 0.81 -0.5
1,604,330 + BT1287 0.81 -0.4
1,604,331 - BT1287 0.81 -0.3
1,604,440 + +0.1
1,604,460 + -0.3
1,604,566 - +0.4
1,604,566 - -0.5
1,604,606 - +0.1
1,604,616 + -0.9
1,604,631 - -0.9
1,604,714 + -1.5
1,604,758 + +0.1
1,604,785 - BT1288 0.10 -0.9
1,604,898 - BT1288 0.18 -1.4
1,604,977 - BT1288 0.24 -0.8
1,605,006 + BT1288 0.26 -1.5
1,605,111 - BT1288 0.34 -0.5
1,605,134 + BT1288 0.36 -2.3
1,605,141 + BT1288 0.36 -2.9
1,605,235 - BT1288 0.43 +1.1
1,605,235 - BT1288 0.43 -0.3
1,605,235 - BT1288 0.43 +0.6
1,605,339 + BT1288 0.51 +0.1
1,605,418 - BT1288 0.57 -1.9
1,605,418 - BT1288 0.57 +1.1
1,605,525 - BT1288 0.65 -1.5
1,605,525 - BT1288 0.65 -2.8
1,605,537 - BT1288 0.66 -0.4
1,605,544 - BT1288 0.66 -1.2
1,605,561 + BT1288 0.67 -1.3
1,605,562 - BT1288 0.68 +0.8
1,605,609 + BT1288 0.71 -0.4
1,605,613 - BT1288 0.71 -1.9
1,605,650 + BT1288 0.74 -1.5
1,605,651 - BT1288 0.74 -2.5
1,605,722 + BT1288 0.79 -0.9
1,605,722 + BT1288 0.79 -1.2
1,605,723 - BT1288 0.79 -2.9
1,605,806 + BT1288 0.86 -0.3
1,605,820 - BT1288 0.87 -1.9
1,605,879 + -0.9
1,605,882 - -0.5
1,605,895 - -0.8
1,605,896 + -1.5
1,605,897 - +1.6
1,605,897 - +0.5
1,605,936 - -0.7
1,605,936 - -0.9
1,606,016 + +1.1
1,606,017 - +0.1
1,606,068 + -0.5
1,606,159 + BT1289 0.17 -0.9
1,606,159 + BT1289 0.17 -0.8
1,606,159 + BT1289 0.17 +0.1
1,606,159 + BT1289 0.17 -0.5
1,606,160 - BT1289 0.17 -0.9
1,606,196 + BT1289 0.22 -2.1
1,606,196 + BT1289 0.22 -0.9
1,606,304 + BT1289 0.35 +0.6
1,606,378 - BT1289 0.44 +0.1
1,606,430 - BT1289 0.51 +1.6
1,606,459 - BT1289 0.55 -0.5
1,606,487 + BT1289 0.58 -1.0
1,606,508 + BT1289 0.61 +0.1
1,606,509 - BT1289 0.61 -1.5
1,606,590 - BT1289 0.71 +0.1
1,606,625 + BT1289 0.75 -0.9
1,606,739 - BT1289 0.90 -0.9
1,606,767 + -0.9
1,606,780 - -1.5
1,606,816 - -0.9
1,606,821 - +2.4
1,606,841 - -0.6
1,606,927 + BT1290 0.15 +2.1
1,606,927 + BT1290 0.15 -1.9
1,606,957 + BT1290 0.18 +1.6

Or see this region's nucleotide sequence